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Literature summary for 1.2.1.5 extracted from

  • Tamaki, N.; Hama, T.
    Aldehyde dehydrogenase from bakers yeast (1982), Methods Enzymol., 89, 469-473.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
207000
-
sucrose density gradient centrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.6
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-25°C, 50 mM potassium phosphate, pH 6.8, 2 mM 2-mercaptoethanol, 1 mM EDTA, 2 mM propionic acid, 0.6 mM PMSF, stable for at least 3 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Saccharomyces cerevisiae acetate + NADH + H+
-
ir
benzaldehyde + NAD+ + H2O
-
Saccharomyces cerevisiae benzoate + NADH + H+
-
?
formaldehyde + NAD+ + H2O
-
Saccharomyces cerevisiae formate + NADH + H+
-
?
glycolaldehyde + NAD+
-
Saccharomyces cerevisiae glycolate + NADH
-
?
hexanal + NAD+ + H2O
-
Saccharomyces cerevisiae hexanoate + NADH + H+
-
?
isobutanal + NAD+ + H2O
-
Saccharomyces cerevisiae isobutyrate + NADH + H+
-
?
malonic semialdehyde + NAD+
-
Saccharomyces cerevisiae ?
-
?
n-butanal + NAD+ + H2O
-
Saccharomyces cerevisiae butanoate + NADH + H+
-
?
n-pentanal + NAD+ + H2O
-
Saccharomyces cerevisiae n-pentanoate + NADH + H+
-
?
propanal + NAD+ + H2O
-
Saccharomyces cerevisiae propionate + NADH + H+
-
ir
succinic semialdehyde + NAD+
-
Saccharomyces cerevisiae ? + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
reaction with propanal and NAD+ or NADP+ Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae
NADP+
-
Saccharomyces cerevisiae