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show all sequences of 1.2.1.49

Biochemical genetics of methylglyoxal dehydrogenases in the laboratory rat (Rattus norvegicus)

Bender, K.; Seibert, R.T.; Wienker, T.F.; Kren, V.; Pravenec, M.; Bissbort, S.; Biochem. Genet. 32, 147-154 (1994)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
detection of three Mgd loci, which show a restrictive tissue expression, Mgd1 and Mgd2 are preferentially expressed in liver and kidney, Mgd3 is expressed apparently exclusively in heart
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADP+ + H2O
Rattus norvegicus
-
pyruvate + NADPH + H+
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
inbred strains, recombinant inbred strains
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoaldehydes + NADP+ + H2O
-
288148
Rattus norvegicus
2-oxo acids + NADPH
-
288148
Rattus norvegicus
?
methylglyoxal + NADP+ + H2O
-
288148
Rattus norvegicus
pyruvate + NADPH + H+
-
288148
Rattus norvegicus
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
Mgd1 and Mgd2 enzymes use also NAD+ as cofactor, see also EC 1.2.1.23
Rattus norvegicus
NADP+
isoenzyme Mgd3 is exclusively NADP+-dependent
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
detection of three Mgd loci, which show a restrictive tissue expression, Mgd1 and Mgd2 are preferentially expressed in liver and kidney, Mgd3 is expressed apparently exclusively in heart
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
Mgd1 and Mgd2 enzymes use also NAD+ as cofactor, see also EC 1.2.1.23
Rattus norvegicus
NADP+
isoenzyme Mgd3 is exclusively NADP+-dependent
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADP+ + H2O
Rattus norvegicus
-
pyruvate + NADPH + H+
-
Rattus norvegicus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoaldehydes + NADP+ + H2O
-
288148
Rattus norvegicus
2-oxo acids + NADPH
-
288148
Rattus norvegicus
?
methylglyoxal + NADP+ + H2O
-
288148
Rattus norvegicus
pyruvate + NADPH + H+
-
288148
Rattus norvegicus
?
Other publictions for EC 1.2.1.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
655211
Vander Jagt
Methylglyoxal metabolism and d ...
Homo sapiens
Chem. Biol. Interact.
143-144
341-351
2003
2
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-
-
-
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1
1
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1
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1
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1
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2
1
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1
1
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2
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-
-
-
-
-
-
-
-
1
1
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-
-
1
-
1
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2
1
-
-
-
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1
1
-
-
-
-
-
-
-
-
288148
Bender
Biochemical genetics of methyl ...
Rattus norvegicus
Biochem. Genet.
32
147-154
1994
-
-
1
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-
-
-
-
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1
-
1
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-
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3
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2
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2
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1
2
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1
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3
-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
288140
Ray
Purification and characterizat ...
Capra hircus
J. Biol. Chem.
257
10566-10570
1982
-
-
-
-
-
-
-
3
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2
1
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1
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1
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1
1
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2
1
-
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1
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1
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1
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3
-
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2
1
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1
-
1
1
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2
1
-
-
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-
1
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-
-
-
-
-
-
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288141
Ray
On the interaction of nucleoti ...
Capra hircus
J. Biol. Chem.
257
10571-10574
1982
-
-
-
-
-
-
1
1
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-
1
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1
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-
-
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2
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-
-
-
-
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-
1
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-
1
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1
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1
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-
1
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-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
288256
Vander Jagt
2-Ketoaldehyde dehydrogenase f ...
Rattus norvegicus
Methods Enzymol.
89
513-515
1982
2
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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1
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1
1
1
2
-
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-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
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1
-
1
1
1
2
-
-
-
-
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-
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288259
Vander Jagt
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
484
260-267
1977
2
-
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-
-
-
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4
-
-
1
1
-
1
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1
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-
1
2
1
2
-
-
-
-
-
1
1
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
4
-
-
1
1
-
-
-
1
-
1
2
1
2
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
288142
Dunkerton
Some observations on the NADP+ ...
Ovis aries
Biochem. J.
153
503-504
1976
1
-
-
-
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-
-
4
-
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1
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
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4
-
-
-
1
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-
-
-
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1
1
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3
-
-
-
-
-
-
-
-
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-
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-
-
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288143
Dunkerton
Purification of 2-oxoaldehyde ...
Ovis aries
Biochem. J.
149
609-617
1975
7
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
1
-
-
1
-
1
4
-
-
-
-
-
1
1
-
2
-
-
-
7
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
1
-
1
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288145
Jellum
Metabolism of the ketoaldehyde ...
Ovis aries, Rattus norvegicus
Biochim. Biophys. Acta
165
357-363
1968
-
-
-
-
-
-
-
2
-
-
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4
-
2
-
-
1
-
-
2
-
-
6
-
-
-
-
-
-
-
-
4
-
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4
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-
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2
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4
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1
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2
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-
6
-
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-
-
-
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-
-
-
-
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-
-
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-
288146
Monder
alpha-Keto aldehyde dehydrogen ...
Ovis aries
J. Biol. Chem.
242
4603-4609
1967
-
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8
3
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1
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1
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1
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2
1
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2
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3
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3
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3
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8
-
3
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-
1
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1
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2
1
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2
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3
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