BRENDA - Enzyme Database show
show all sequences of 1.2.1.49

Purification of 2-oxoaldehyde dehydrogenase and its dependence on unusual amines

Dunkerton, J.; James, S.P.; Biochem. J. 149, 609-617 (1975)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-amino-2-methylpropan-1-ol
activation of aldehyde oxidation
Ovis aries
2-amino-2-methylpropane-1,3-diol
activation of aldehyde oxidation
Ovis aries
2-Aminobutan-1-ol
activation of aldehyde oxidation
Ovis aries
2-aminopropane
activation of aldehyde oxidation
Ovis aries
2-aminopropane-1,3-diol
activation of aldehyde oxidation
Ovis aries
L-2-Aminopropan-1-ol
activation of aldehyde oxidation
Ovis aries
Tris
activation of aldehyde oxidation
Ovis aries
Inhibitors
Inhibitors
Commentary
Organism
Structure
Barbiturates
-
Ovis aries
Nitro-alcohols
-
Ovis aries
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADP+ + H2O
Ovis aries
-
pyruvate + NADPH + H+
-
Ovis aries
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries
-
-
-
Purification (Commentary)
Commentary
Organism
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-200, hydroxylapatite, and DEAE-cellulose
Ovis aries
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries
-
Storage Stability
Storage Stability
Organism
4°C, pH 7.4, 50 mM NaCl, 5 mM 2-amino-2-methylpropane-1,3-diol, 20 days
Ovis aries
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoaldehydes + NADP+ + H2O
enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23
288143
Ovis aries
2-oxo acids + NADPH
-
288143
Ovis aries
?
methylglyoxal + NADP+ + H2O
-
288143
Ovis aries
pyruvate + NADPH + H+
-
288143
Ovis aries
?
additional information
the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme
288143
Ovis aries
?
-
-
-
-
additional information
the presence of amines like Tris, 2-amino-2-methylpropane-1,3-diol, 2-amino-2-methylpropan-1-ol, 2-aminopropane-1,3-diol, 2-aminopropane or 2-aminobutan-1-ol is necessary to activate the enzyme
288143
Ovis aries
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Ovis aries
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.8
-
not active below
Ovis aries
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
enzyme from sheep or rat liver, see also EC 1.2.1.23
Ovis aries
NADP+
-
Ovis aries
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-amino-2-methylpropan-1-ol
activation of aldehyde oxidation
Ovis aries
2-amino-2-methylpropane-1,3-diol
activation of aldehyde oxidation
Ovis aries
2-Aminobutan-1-ol
activation of aldehyde oxidation
Ovis aries
2-aminopropane
activation of aldehyde oxidation
Ovis aries
2-aminopropane-1,3-diol
activation of aldehyde oxidation
Ovis aries
L-2-Aminopropan-1-ol
activation of aldehyde oxidation
Ovis aries
Tris
activation of aldehyde oxidation
Ovis aries
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
enzyme from sheep or rat liver, see also EC 1.2.1.23
Ovis aries
NADP+
-
Ovis aries
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Barbiturates
-
Ovis aries
Nitro-alcohols
-
Ovis aries
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADP+ + H2O
Ovis aries
-
pyruvate + NADPH + H+
-
Ovis aries
?
Purification (Commentary) (protein specific)
Commentary
Organism
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-200, hydroxylapatite, and DEAE-cellulose
Ovis aries
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, pH 7.4, 50 mM NaCl, 5 mM 2-amino-2-methylpropane-1,3-diol, 20 days
Ovis aries
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoaldehydes + NADP+ + H2O
enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23
288143
Ovis aries
2-oxo acids + NADPH
-
288143
Ovis aries
?
methylglyoxal + NADP+ + H2O
-
288143
Ovis aries
pyruvate + NADPH + H+
-
288143
Ovis aries
?
additional information
the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme
288143
Ovis aries
?
-
-
-
-
additional information
the presence of amines like Tris, 2-amino-2-methylpropane-1,3-diol, 2-amino-2-methylpropan-1-ol, 2-aminopropane-1,3-diol, 2-aminopropane or 2-aminobutan-1-ol is necessary to activate the enzyme
288143
Ovis aries
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Ovis aries
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.8
-
not active below
Ovis aries
Other publictions for EC 1.2.1.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
655211
Vander Jagt
Methylglyoxal metabolism and d ...
Homo sapiens
Chem. Biol. Interact.
143-144
341-351
2003
2
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
2
1
-
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288148
Bender
Biochemical genetics of methyl ...
Rattus norvegicus
Biochem. Genet.
32
147-154
1994
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288140
Ray
Purification and characterizat ...
Capra hircus
J. Biol. Chem.
257
10566-10570
1982
-
-
-
-
-
-
-
3
-
-
2
1
-
1
-
-
1
-
-
1
1
-
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
2
1
-
-
-
1
-
1
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288141
Ray
On the interaction of nucleoti ...
Capra hircus
J. Biol. Chem.
257
10571-10574
1982
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288256
Vander Jagt
2-Ketoaldehyde dehydrogenase f ...
Rattus norvegicus
Methods Enzymol.
89
513-515
1982
2
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288259
Vander Jagt
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
484
260-267
1977
2
-
-
-
-
-
-
4
-
-
1
1
-
1
-
-
1
-
-
1
2
1
2
-
-
-
-
-
1
1
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
4
-
-
1
1
-
-
-
1
-
1
2
1
2
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
288142
Dunkerton
Some observations on the NADP+ ...
Ovis aries
Biochem. J.
153
503-504
1976
1
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288143
Dunkerton
Purification of 2-oxoaldehyde ...
Ovis aries
Biochem. J.
149
609-617
1975
7
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
1
-
-
1
-
1
4
-
-
-
-
-
1
1
-
2
-
-
-
7
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
1
-
1
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288145
Jellum
Metabolism of the ketoaldehyde ...
Ovis aries, Rattus norvegicus
Biochim. Biophys. Acta
165
357-363
1968
-
-
-
-
-
-
-
2
-
-
-
4
-
2
-
-
1
-
-
2
-
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
-
4
-
-
-
1
-
2
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288146
Monder
alpha-Keto aldehyde dehydrogen ...
Ovis aries
J. Biol. Chem.
242
4603-4609
1967
-
-
-
-
-
-
8
3
-
-
-
1
-
1
-
-
1
-
-
2
1
-
2
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
3
-
-
-
-
8
-
3
-
-
-
1
-
-
-
1
-
2
1
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
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