Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha and BL21 (DE3) cells | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | - |
Pseudomonas sp. | |
5,5'-dithiobis-(2-nitrobenzoic acid) | - |
Pseudomonas sp. | |
Ba2+ | - |
Pseudomonas sp. | |
Co2+ | - |
Pseudomonas sp. | |
Cu2+ | - |
Pseudomonas sp. | |
Hg2+ | - |
Pseudomonas sp. | |
iodoacetoamide | - |
Pseudomonas sp. | |
K+ | - |
Pseudomonas sp. | |
Li+ | - |
Pseudomonas sp. | |
additional information | potassium fluoride, sodium azide, 2,2'-dipyridyl, and EDTA have no effect on the enzyme activity | Pseudomonas sp. | |
Semicarbazide | - |
Pseudomonas sp. | |
Zn2+ | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
4-N-trimethylaminobutyraldehyde | at pH 9.5 and 30°C | Pseudomonas sp. | |
0.62 | - |
4-dimethylaminobutyraldehyde | at pH 9.5 and 30°C | Pseudomonas sp. | |
1.16 | - |
NAD+ | at pH 9.5 and 30°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Rb+, Na+, Mg2+, and Ca2+ have no effect on the enzyme activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
3 * 52000, SDS-PAGE | Pseudomonas sp. |
150000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-N-trimethylaminobutyraldehyde + NAD+ + H2O | Pseudomonas sp. | - |
gamma-butyrobetaine + NADH + H+ | - |
? | |
4-N-trimethylaminobutyraldehyde + NAD+ + H2O | Pseudomonas sp. 13CM | - |
gamma-butyrobetaine + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | N0DT23 | - |
- |
Pseudomonas sp. 13CM | N0DT23 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and 5'-AMP Sepharose column chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
cell free extract, at pH 9.5 and 30°C | Pseudomonas sp. |
12.4 | - |
native enzyme, after 19.5fold purification, at pH 9.5 and 30°C | Pseudomonas sp. |
15.4 | - |
recombinant enzyme, after 19.5fold purification, at pH 9.5 and 30°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-dimethylaminobutyraldehyde + NAD+ + H2O | - |
Pseudomonas sp. | ? + NADH + H+ | - |
? | |
4-dimethylaminobutyraldehyde + NAD+ + H2O | - |
Pseudomonas sp. 13CM | ? + NADH + H+ | - |
? | |
4-N-trimethylaminobutyraldehyde + NAD+ + H2O | - |
Pseudomonas sp. | gamma-butyrobetaine + NADH + H+ | - |
? | |
4-N-trimethylaminobutyraldehyde + NAD+ + H2O | - |
Pseudomonas sp. 13CM | gamma-butyrobetaine + NADH + H+ | - |
? | |
additional information | no activity with NADP+, trimethylaminobutanol, 3-N-trimethylaminopropionaldehyde, betaine aldehyde, 4-aminobutyraldehyde, and other aliphatic aldehydes such as acetaldehyde, propionaldehyde, butyraldehyde, pivaleraldehyde, isovaleraldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde | Pseudomonas sp. | ? | - |
? | |
additional information | no activity with NADP+, trimethylaminobutanol, 3-N-trimethylaminopropionaldehyde, betaine aldehyde, 4-aminobutyraldehyde, and other aliphatic aldehydes such as acetaldehyde, propionaldehyde, butyraldehyde, pivaleraldehyde, isovaleraldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde | Pseudomonas sp. 13CM | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 52000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
4-N-trimethylaminobutyraldehyde dehydrogenase II | - |
Pseudomonas sp. |
TMABaldehyde-DH II | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | the enzyme is stable up to 25°C while incubation for 30 min at 30°C and above leads to rapid inactivation | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | after pre-incubation for 15 min at 30°C, the enzyme is stable between pH 8.0 and 9.0 but is inactivated completely at pH 10.0 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing | - |
4.5 |