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Literature summary for 1.2.1.47 extracted from

  • Bari, M.R.; Hassan, M.; Akai, N.; Arima, J.; Mori, N.
    Gene cloning and biochemical characterization of 4-N-trimethylaminobutyraldehyde dehydrogenase II from Pseudomonas sp. 13CM (2013), World J. Microbiol. Biotechnol., 29, 683-692.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha and BL21 (DE3) cells Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
Pseudomonas sp.
5,5'-dithiobis-(2-nitrobenzoic acid)
-
Pseudomonas sp.
Ba2+
-
Pseudomonas sp.
Co2+
-
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
iodoacetoamide
-
Pseudomonas sp.
K+
-
Pseudomonas sp.
Li+
-
Pseudomonas sp.
additional information potassium fluoride, sodium azide, 2,2'-dipyridyl, and EDTA have no effect on the enzyme activity Pseudomonas sp.
Semicarbazide
-
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
4-N-trimethylaminobutyraldehyde at pH 9.5 and 30°C Pseudomonas sp.
0.62
-
4-dimethylaminobutyraldehyde at pH 9.5 and 30°C Pseudomonas sp.
1.16
-
NAD+ at pH 9.5 and 30°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Rb+, Na+, Mg2+, and Ca2+ have no effect on the enzyme activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
3 * 52000, SDS-PAGE Pseudomonas sp.
150000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-N-trimethylaminobutyraldehyde + NAD+ + H2O Pseudomonas sp.
-
gamma-butyrobetaine + NADH + H+
-
?
4-N-trimethylaminobutyraldehyde + NAD+ + H2O Pseudomonas sp. 13CM
-
gamma-butyrobetaine + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. N0DT23
-
-
Pseudomonas sp. 13CM N0DT23
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and 5'-AMP Sepharose column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
cell free extract, at pH 9.5 and 30°C Pseudomonas sp.
12.4
-
native enzyme, after 19.5fold purification, at pH 9.5 and 30°C Pseudomonas sp.
15.4
-
recombinant enzyme, after 19.5fold purification, at pH 9.5 and 30°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-dimethylaminobutyraldehyde + NAD+ + H2O
-
Pseudomonas sp. ? + NADH + H+
-
?
4-dimethylaminobutyraldehyde + NAD+ + H2O
-
Pseudomonas sp. 13CM ? + NADH + H+
-
?
4-N-trimethylaminobutyraldehyde + NAD+ + H2O
-
Pseudomonas sp. gamma-butyrobetaine + NADH + H+
-
?
4-N-trimethylaminobutyraldehyde + NAD+ + H2O
-
Pseudomonas sp. 13CM gamma-butyrobetaine + NADH + H+
-
?
additional information no activity with NADP+, trimethylaminobutanol, 3-N-trimethylaminopropionaldehyde, betaine aldehyde, 4-aminobutyraldehyde, and other aliphatic aldehydes such as acetaldehyde, propionaldehyde, butyraldehyde, pivaleraldehyde, isovaleraldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde Pseudomonas sp. ?
-
?
additional information no activity with NADP+, trimethylaminobutanol, 3-N-trimethylaminopropionaldehyde, betaine aldehyde, 4-aminobutyraldehyde, and other aliphatic aldehydes such as acetaldehyde, propionaldehyde, butyraldehyde, pivaleraldehyde, isovaleraldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde Pseudomonas sp. 13CM ?
-
?

Subunits

Subunits Comment Organism
homotrimer 3 * 52000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
4-N-trimethylaminobutyraldehyde dehydrogenase II
-
Pseudomonas sp.
TMABaldehyde-DH II
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 30 the enzyme is stable up to 25°C while incubation for 30 min at 30°C and above leads to rapid inactivation Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 after pre-incubation for 15 min at 30°C, the enzyme is stable between pH 8.0 and 9.0 but is inactivated completely at pH 10.0 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
4.5