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Literature summary for 1.2.1.47 extracted from

  • Vaz, F.M.; Fouchier, S.W.; Ofman, R.; Sommer, M.; Wanders, R.J.A.
    Molecular and biochemical characterization of rat gamma-trimethylaminobutyraldehyde dehydrogenase and evidence for the involvement of human aldehyde dehydrogenase 9 in carnitine biosynthesis (2000), J. Biol. Chem., 275, 7390-7394.
    View publication on PubMed
Search for more literature for 1.2.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 4-trimethylaminobutyraldehyde
-
 Rattus norvegicus
0.006
-
 octanal
-
 Rattus norvegicus
0.007
-
 Heptanal
-
 Rattus norvegicus
0.0088
-
 hexadecanal
-
 Rattus norvegicus
0.009
-
 hexanal
-
 Rattus norvegicus
0.01
-
 pentanal
-
 Rattus norvegicus
0.013
-
 Butyraldehyde
-
 Rattus norvegicus
0.016
-
 propionaldehyde
-
 Rattus norvegicus
0.024
-
 4-Aminobutyraldehyde
-
 Rattus norvegicus
0.028
-
 NAD+
-
 Rattus norvegicus
0.102
-
 acetaldehyde
-
 Rattus norvegicus
0.123
-
 Betaine aldehyde
-
 Rattus norvegicus
1.63
-
 NADP+
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-trimethylaminobutyraldehyde + NAD+ Rattus norvegicus
-
 4-trimethylaminobutyrate + NADH + H+
-
 ?
4-trimethylaminobutyraldehyde + NAD+ Rattus norvegicus Wistar
-
 4-trimethylaminobutyrate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q9JLJ3 Wistar strain
-
Rattus norvegicus Wistar Q9JLJ3 Wistar strain
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography on SP-Sepharose, Red Sepharose CL-6B and Hydroxylapatite Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.772
-
 purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 1 mM dithiothreitol, glycerol, several months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-trimethylaminobutyraldehyde + NAD+
-
 Rattus norvegicus 4-trimethylaminobutyrate + NADH + H+
-
 ?
4-trimethylaminobutyraldehyde + NAD+
-
 Rattus norvegicus Wistar 4-trimethylaminobutyrate + NADH + H+
-
 ?
4-trimethylaminobutyraldehyde + NADP+ + H2O
-
 Rattus norvegicus 4-trimethylaminobutyrate + NADPH + H+
-
 r
acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus acetate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus Wistar acetate + NADH + H+
-
 ?
betaine aldehyde + NAD+
-
 Rattus norvegicus betaine + NADH
-
 ?
butyraldehyde + NAD+
-
 Rattus norvegicus butyrate + NADH
-
 ?
butyraldehyde + NAD+
-
 Rattus norvegicus Wistar butyrate + NADH
-
 ?
gamma-aminobutyraldehyde + NAD+
-
 Rattus norvegicus gamma-aminobutyrate + NADH
-
 ?
gamma-aminobutyraldehyde + NAD+
-
 Rattus norvegicus Wistar gamma-aminobutyrate + NADH
-
 ?
heptanal + NAD+
-
 Rattus norvegicus heptanoate + NADH
-
 ?
hexadecanal + NAD+
-
 Rattus norvegicus hexadecanoate + NADH
-
 ?
hexanal + NAD+ + H2O
-
 Rattus norvegicus hexanoate + NADH + H+
-
 ?
octanal + NAD+ + H2O
-
 Rattus norvegicus octanoate + NADH + H+
-
 ?
pentanal + NAD+
-
 Rattus norvegicus pentanoate + NADH
-
 ?
propionaldehyde + NAD+
-
 Rattus norvegicus propionate + NADH
-
 ?
propionaldehyde + NAD+
-
 Rattus norvegicus Wistar propionate + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rattus norvegicus
NADP+ can also be used as oxidant Rattus norvegicus