Application | Comment | Organism |
---|---|---|
industry | development of a bi-enzyme biosensor for formaldehyde using diaphorase from Bacillus stearothermophilus, NAD+- and glutathione-dependent FDH as bio-recognition elements. Both enzymes can be immobilized on the top of a graphite electrode together with two osmium-redox polymers. Characteristics of the optimized formaldehyde biosensor are sensitivity, detection limit, and linear dynamic range | Ogataea angusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
- |
- |
Ogataea angusta Tf 11-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ion-exchange chromatography | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.9 | - |
- |
Ogataea angusta |
Storage Stability | Organism |
---|---|
-10°C | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | - |
Ogataea angusta | formate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Ogataea angusta Tf 11-6 | formate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Ogataea angusta |
NAD+- and glutathione-dependent formaldehyde dehydrogenase | - |
Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
maximum response of the biosensor | Ogataea angusta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
FDH is not stable at a pH below 6.5, but shows higher stability in an alkaline pH domain | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Ogataea angusta | |
NAD+ | - |
Ogataea angusta |