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Literature summary for 1.2.1.44 extracted from

  • Park, H.L.; Bhoo, S.H.; Kwon, M.; Lee, S.W.; Cho, M.H.
    Biochemical and expression analyses of the rice cinnamoyl-CoA reductase gene family (2017), Front. Plant Sci., 8, 2099 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment) Organism
isoforms CCR17, CCR19, CCR20, and CCR21 are expressed in Escherichia coli BL21(DE3) cells Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 feruloyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.0102
-
 sinapoyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.01571
-
 feruloyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.01636
-
 4-coumaroyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.02334
-
 sinapoyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.02408
-
 4-coumaroyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.02685
-
 feruloyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.03666
-
 4-coumaroyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.06254
-
 sinapoyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamoyl-CoA + NADPH + H+ Oryza sativa Japonica Group
-
 cinnamaldehyde + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group
-
 cultivar Dongjin
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Oryza sativa Japonica Group

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa Japonica Group
-
panicle highest expression of isoform CCR19 Oryza sativa Japonica Group
-
root highest expression of isoform CCR20 and CCR21 Oryza sativa Japonica Group
-
shoot highest expression of isoform CCR17 Oryza sativa Japonica Group
-
stem
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+
-
 Oryza sativa Japonica Group 4-coumaraldehyde + CoA + NADP+
-
 ?
cinnamoyl-CoA + NADPH + H+
-
 Oryza sativa Japonica Group cinnamaldehyde + CoA + NADP+
-
 ?
feruloyl-CoA + NADPH + H+
-
 Oryza sativa Japonica Group coniferaldehyde + CoA + NADP+
-
 ?
sinapoyl-CoA + NADPH + H+
-
 Oryza sativa Japonica Group sinapaldehyde + CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 37900, isoform CCR17, calculated from amino acid sequence Oryza sativa Japonica Group
? x * 37900, isoform CCR21, calculated from amino acid sequence Oryza sativa Japonica Group
? x * 38600, isoform CCR19, calculated from amino acid sequence Oryza sativa Japonica Group
? x * 38700, isoform CCR20, calculated from amino acid sequence Oryza sativa Japonica Group

Synonyms

Synonyms Comment Organism
CCR17 isoform Oryza sativa Japonica Group
CCR19 isoform Oryza sativa Japonica Group
CCR20 isoform Oryza sativa Japonica Group
CCR21 isoform Oryza sativa Japonica Group

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 sinapoyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.019
-
 4-coumaroyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.027
-
 feruloyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.094
-
 sinapoyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.129
-
 4-coumaroyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.191
-
 feruloyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.353
-
 4-coumaroyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.377
-
 feruloyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.57
-
 sinapoyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Oryza sativa Japonica Group

Expression

Organism Comment Expression
Oryza sativa Japonica Group the expressions of isoforms CCR17 and 21 are induced in response to biotic and abiotic stresses, such as Magnaporthe grisea and Xanthomonas oryzae pv. oryzae infections, UV-irradiation and high salinity (250 mM NaCl) up

General Information

General Information Comment Organism
physiological function isoform CCR20 is primarily involved in developmental deposition of lignins in secondary cell walls Oryza sativa Japonica Group

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17
-
 sinapoyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.33
-
 4-coumaroyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
4
-
 sinapoyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
5.33
-
 4-coumaroyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group
7.17
-
 feruloyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group
9.17
-
 sinapoyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group
10
-
 4-coumaroyl-CoA isoform CCR19, at pH 6.3 and 30°C Oryza sativa Japonica Group
12.8
-
 feruloyl-CoA isoform CCR21, at pH 6.3 and 30°C Oryza sativa Japonica Group
23.5
-
 feruloyl-CoA isoform CCR20, at pH 6.3 and 30°C Oryza sativa Japonica Group