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Literature summary for 1.2.1.44 extracted from

  • Sonawane, P.; Vishwakarma, R.K.; Singh, S.; Gaikwad, S.; Khan, B.M.
    Steady state fluorescence studies of wild type recombinant cinnamoyl CoA reductase (Ll-CCRH1) and its active site mutants (2014), J. Fluoresc., 24, 665-673 .
    View publication on PubMed
Search for more literature for 1.2.1.44

Protein Variants

Protein Variants Comment Organism
D77G site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
F30V site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
H215L site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
I31N site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
K174M site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
R51G site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
S136A site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
S212G site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
V200E site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
Y170H site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl-CoA + NADPH + H+ Leucaena leucocephala
-
 coniferaldehyde + CoA + NADP+
-
 r
sinapoyl-CoA + NADPH + H+ Leucaena leucocephala
-
 sinapaldehyde + CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala Q08GL0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloyl-CoA + NADPH + H+
-
 Leucaena leucocephala coniferaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+ best substrate Leucaena leucocephala coniferaldehyde + CoA + NADP+
-
 r
sinapoyl-CoA + NADPH + H+
-
 Leucaena leucocephala sinapaldehyde + CoA + NADP+
-
 r

Synonyms

Synonyms Comment Organism
CCR
-
 Leucaena leucocephala
cinnamoyl CoA reductase
-
 Leucaena leucocephala
cinnamoyl CoA reductase 1
-
 Leucaena leucocephala
Ll-CCRH1
-
 Leucaena leucocephala

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Leucaena leucocephala
NADPH
-
 Leucaena leucocephala

General Information

General Information Comment Organism
additional information active site characterization of Ll-CCRH1 by modeling/docking, site directed mutagenesis and chemical modification studies, conformational transitions of Ll-CCRH1 are studied using fluorescence and circualar dichroism spectroscopy, overview. Native Ll-CCRH1 is a multi tryptophan protein, the active site of Ll-CCRH1 is made up of 10 residues, that is, Phe30, Ile31, Arg51, Asp77, Ser136, Tyr170, Lys174, Val200, Ser212, and His215 Leucaena leucocephala