Protein Variants | Comment | Organism |
---|---|---|
D77G | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
F30V | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
H215L | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
I31N | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
K174M | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
R51G | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
S136A | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
S212G | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
V200E | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
Y170H | site-directed mutagenesis, structure comparison to the wild-type enzyme | Leucaena leucocephala |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA + NADPH + H+ | Leucaena leucocephala | - |
coniferaldehyde + CoA + NADP+ | - |
r | |
sinapoyl-CoA + NADPH + H+ | Leucaena leucocephala | - |
sinapaldehyde + CoA + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucaena leucocephala | Q08GL0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA + NADPH + H+ | - |
Leucaena leucocephala | coniferaldehyde + CoA + NADP+ | - |
r | |
feruloyl-CoA + NADPH + H+ | best substrate | Leucaena leucocephala | coniferaldehyde + CoA + NADP+ | - |
r | |
sinapoyl-CoA + NADPH + H+ | - |
Leucaena leucocephala | sinapaldehyde + CoA + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CCR | - |
Leucaena leucocephala |
cinnamoyl CoA reductase | - |
Leucaena leucocephala |
cinnamoyl CoA reductase 1 | - |
Leucaena leucocephala |
Ll-CCRH1 | - |
Leucaena leucocephala |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Leucaena leucocephala | |
NADPH | - |
Leucaena leucocephala |
General Information | Comment | Organism |
---|---|---|
additional information | active site characterization of Ll-CCRH1 by modeling/docking, site directed mutagenesis and chemical modification studies, conformational transitions of Ll-CCRH1 are studied using fluorescence and circualar dichroism spectroscopy, overview. Native Ll-CCRH1 is a multi tryptophan protein, the active site of Ll-CCRH1 is made up of 10 residues, that is, Phe30, Ile31, Arg51, Asp77, Ser136, Tyr170, Lys174, Val200, Ser212, and His215 | Leucaena leucocephala |