BRENDA - Enzyme Database show
show all sequences of 1.2.1.44

In silico mutagenesis and docking studies of active site residues suggest altered substrate specificity and possible physiological role of cinnamoyl CoA reductase 1 (Ll-CCRH1)

Sonawane, P.; Patel, K.; Vishwakarma, R.K.; Singh, S.; Khan, B.M.; Bioinformation 9, 224-232 (2013)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D77A
the mutant shows specificity towards coumaroyl-CoA
Leucaena leucocephala
D77G
the mutant displays equal affinity for caffeoyl-CoA and sinapoyl-CoA
Leucaena leucocephala
D77N
the mutant has same substrate affinity (feruloyl CoA) as that of wild type enzyme
Leucaena leucocephala
F30L
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
F30S
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
F30V
the mutant prefers feruloyl-CoA as favoured substrate
Leucaena leucocephala
F30Y
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
H215L
the mutant displays preference for sinapoyl-CoA
Leucaena leucocephala
H215R
coumaroyl-CoA is specific for mutant H215R
Leucaena leucocephala
H215Y
5-hydroxyferuloyl-CoA is specific for mutant H215Y
Leucaena leucocephala
I31F
the mutant shows more negative binding energy for hydroxyferuloyl CoA
Leucaena leucocephala
I31M
the mutant exhibits equal affinity for coumaroyl and hydroxyferulol CoA
Leucaena leucocephala
I31N
the mutant demonstrates better affinity for sinapoyl CoA over others
Leucaena leucocephala
K174E
the mutant shows coumaroyl-CoA as preferred substrate
Leucaena leucocephala
K174M
the mutant is specific for feruloyl CoA
Leucaena leucocephala
K174N
the mutant has favorable binding energy for hydroxyferuloyl-CoA
Leucaena leucocephala
K174R
the mutant has favorable binding energy for hydroxyferuloyl-CoA
Leucaena leucocephala
K174T
the mutant shows coumaroyl-CoA as preferred substrate
Leucaena leucocephala
R51G
the mutation alters side chain from polar charged to small compact neutral residue, resulting in marked decrease in accessible surface area and leads to loss of interactions with substrate
Leucaena leucocephala
R51K
the mutant shows affinity towards caffeoyl-CoA
Leucaena leucocephala
S136A
coumaroyl CoA is a better substrate for mutant S136A
Leucaena leucocephala
S136C
coumaroyl CoA is a better substrate for mutant S136C
Leucaena leucocephala
S136P
the mutant shows favored specificity for feruloyl-CoA
Leucaena leucocephala
S136T
the mutant shows favored specificity for feruloyl-CoA
Leucaena leucocephala
S136Y
the mutant shows preference for caffeoyl-CoA
Leucaena leucocephala
S212T
the mutant shows feruloyl CoA as promising substrate
Leucaena leucocephala
V200A
the mutant displays substrate specificity towards coumaroyl-CoA
Leucaena leucocephala
V200G
the mutant displays substrate specificity towards coumaroyl-CoA
Leucaena leucocephala
V200M
the mutant exhibits increased affinity for coumaroyl-CoA
Leucaena leucocephala
Y170C
the mutant displays less number of interactions compared to the wild type enzyme
Leucaena leucocephala
Y170F
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
Y170H
the mutant demonstrates affinity for feruloyl-CoA
Leucaena leucocephala
Y170N
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-coumaroyl-CoA + NADPH + H+
Leucaena leucocephala
-
4-coumaric aldehyde + CoA + NADP+
-
-
?
5-hydroxyferuloyl-CoA + NADPH + H+
Leucaena leucocephala
-
5-hydroxyconiferaldehyde + CoA + NADP+
-
-
?
caffeoyl-CoA + NADPH + H+
Leucaena leucocephala
-
caffealdehyde + CoA + NADP+
-
-
?
cinnamaldehyde + CoA + NADP+
Leucaena leucocephala
-
cinnamoyl-CoA + NADPH + H+
-
-
?
feruloyl-CoA + NADPH + H+
Leucaena leucocephala
most favoured substrate
coniferaldehyde + CoA + NADP+
-
-
?
sinapoyl-CoA + NADPH + H+
Leucaena leucocephala
-
sinapaldehyde + CoA + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leucaena leucocephala
Q08GL0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
4-coumaric aldehyde + CoA + NADP+
-
-
-
?
5-hydroxyferuloyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
5-hydroxyconiferaldehyde + CoA + NADP+
-
-
-
?
caffeoyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
caffealdehyde + CoA + NADP+
-
-
-
?
cinnamaldehyde + CoA + NADP+
-
724494
Leucaena leucocephala
cinnamoyl-CoA + NADPH + H+
-
-
-
?
feruloyl-CoA + NADPH + H+
most favoured substrate
724494
Leucaena leucocephala
coniferaldehyde + CoA + NADP+
-
-
-
?
sinapoyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
sinapaldehyde + CoA + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Leucaena leucocephala
NADPH
-
Leucaena leucocephala
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Leucaena leucocephala
NADPH
-
Leucaena leucocephala
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D77A
the mutant shows specificity towards coumaroyl-CoA
Leucaena leucocephala
D77G
the mutant displays equal affinity for caffeoyl-CoA and sinapoyl-CoA
Leucaena leucocephala
D77N
the mutant has same substrate affinity (feruloyl CoA) as that of wild type enzyme
Leucaena leucocephala
F30L
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
F30S
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
F30V
the mutant prefers feruloyl-CoA as favoured substrate
Leucaena leucocephala
F30Y
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
H215L
the mutant displays preference for sinapoyl-CoA
Leucaena leucocephala
H215R
coumaroyl-CoA is specific for mutant H215R
Leucaena leucocephala
H215Y
5-hydroxyferuloyl-CoA is specific for mutant H215Y
Leucaena leucocephala
I31F
the mutant shows more negative binding energy for hydroxyferuloyl CoA
Leucaena leucocephala
I31M
the mutant exhibits equal affinity for coumaroyl and hydroxyferulol CoA
Leucaena leucocephala
I31N
the mutant demonstrates better affinity for sinapoyl CoA over others
Leucaena leucocephala
K174E
the mutant shows coumaroyl-CoA as preferred substrate
Leucaena leucocephala
K174M
the mutant is specific for feruloyl CoA
Leucaena leucocephala
K174N
the mutant has favorable binding energy for hydroxyferuloyl-CoA
Leucaena leucocephala
K174R
the mutant has favorable binding energy for hydroxyferuloyl-CoA
Leucaena leucocephala
K174T
the mutant shows coumaroyl-CoA as preferred substrate
Leucaena leucocephala
R51G
the mutation alters side chain from polar charged to small compact neutral residue, resulting in marked decrease in accessible surface area and leads to loss of interactions with substrate
Leucaena leucocephala
R51K
the mutant shows affinity towards caffeoyl-CoA
Leucaena leucocephala
S136A
coumaroyl CoA is a better substrate for mutant S136A
Leucaena leucocephala
S136C
coumaroyl CoA is a better substrate for mutant S136C
Leucaena leucocephala
S136P
the mutant shows favored specificity for feruloyl-CoA
Leucaena leucocephala
S136T
the mutant shows favored specificity for feruloyl-CoA
Leucaena leucocephala
S136Y
the mutant shows preference for caffeoyl-CoA
Leucaena leucocephala
S212T
the mutant shows feruloyl CoA as promising substrate
Leucaena leucocephala
V200A
the mutant displays substrate specificity towards coumaroyl-CoA
Leucaena leucocephala
V200G
the mutant displays substrate specificity towards coumaroyl-CoA
Leucaena leucocephala
V200M
the mutant exhibits increased affinity for coumaroyl-CoA
Leucaena leucocephala
Y170C
the mutant displays less number of interactions compared to the wild type enzyme
Leucaena leucocephala
Y170F
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
Y170H
the mutant demonstrates affinity for feruloyl-CoA
Leucaena leucocephala
Y170N
the mutant shows preference for coumaroyl-CoA
Leucaena leucocephala
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-coumaroyl-CoA + NADPH + H+
Leucaena leucocephala
-
4-coumaric aldehyde + CoA + NADP+
-
-
?
5-hydroxyferuloyl-CoA + NADPH + H+
Leucaena leucocephala
-
5-hydroxyconiferaldehyde + CoA + NADP+
-
-
?
caffeoyl-CoA + NADPH + H+
Leucaena leucocephala
-
caffealdehyde + CoA + NADP+
-
-
?
cinnamaldehyde + CoA + NADP+
Leucaena leucocephala
-
cinnamoyl-CoA + NADPH + H+
-
-
?
feruloyl-CoA + NADPH + H+
Leucaena leucocephala
most favoured substrate
coniferaldehyde + CoA + NADP+
-
-
?
sinapoyl-CoA + NADPH + H+
Leucaena leucocephala
-
sinapaldehyde + CoA + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
4-coumaric aldehyde + CoA + NADP+
-
-
-
?
5-hydroxyferuloyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
5-hydroxyconiferaldehyde + CoA + NADP+
-
-
-
?
caffeoyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
caffealdehyde + CoA + NADP+
-
-
-
?
cinnamaldehyde + CoA + NADP+
-
724494
Leucaena leucocephala
cinnamoyl-CoA + NADPH + H+
-
-
-
?
feruloyl-CoA + NADPH + H+
most favoured substrate
724494
Leucaena leucocephala
coniferaldehyde + CoA + NADP+
-
-
-
?
sinapoyl-CoA + NADPH + H+
-
724494
Leucaena leucocephala
sinapaldehyde + CoA + NADP+
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme carries out the first committed step in monolignol biosynthesis and acts as a first regulatory point in lignin formation
Leucaena leucocephala
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme carries out the first committed step in monolignol biosynthesis and acts as a first regulatory point in lignin formation
Leucaena leucocephala
Other publictions for EC 1.2.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743501
Sattler
Structural and biochemical ch ...
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Plant Physiol.
173
1031-1044
2017
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9
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4
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743559
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Characterization of the cinna ...
Populus tomentosa
Planta
245
61-75
2017
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1
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9
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16
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5
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3
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7
1
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9
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17
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7
2
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2
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15
2
2
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2
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3
5
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5
5
743405
Li
Cloning and expression analys ...
Sorghum bicolor
PeerJ
4
e2005
2016
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1
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1
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15
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6
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3
1
2
6
3
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743161
Ghosh
-
Developmental- and stress-med ...
Hibiscus cannabinus, Hibiscus cannabinus C-9
J. Plant Interact.
10
158-166
2015
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1
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2
2
3
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743233
Liu
Overexpression of artificiall ...
Populus tomentosa
Microb. Cell Fact.
14
118
2015
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1
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1
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-
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4
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1
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8
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8
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1
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743422
Srivastava
Abiotic stress induces change ...
Leucaena leucocephala
Physiol. Mol. Biol. Plants
21
197-205
2015
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2
1
1
2
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743427
Zhang
Functional characterization o ...
Betula platyphylla x Betula pendula
Physiol. Plant.
154
283-296
2015
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1
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1
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4
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3
3
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743470
Xue
CCR1, an enzyme required for ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant J.
83
375-387
2015
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1
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1
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4
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24
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2
1
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742589
Yeh
FaPOD27 functions in the meta ...
Fragaria x ananassa
Front. Plant Sci.
5
518
2014
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1
3
3
742677
Sonawane
Conformational transitions of ...
Leucaena leucocephala
Int. J. Biol. Macromol.
64
30-35
2014
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743007
Sonawane
Steady state fluorescence stu ...
Leucaena leucocephala
J. Fluoresc.
24
665-673
2014
-
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10
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2
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2
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10
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1
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743156
Han
-
Preparation of cinnamoyl-CoA ...
Populus tomentosa
J. Plant Biochem. Biotechnol.
23
348-357
2014
-
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1
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4
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5
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1
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1
1
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1
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1
1
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1
1
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743447
Pan
Structural studies of cinnamo ...
Medicago truncatula, Petunia x hybrida
Plant Cell
26
3709-3727
2014
-
4
1
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3
1
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11
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6
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1
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19
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1
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2
3
1
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4
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6
1
6
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3
1
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11
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1
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19
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1
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3
1
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4
6
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3
3
743683
Van Acker
Improved saccharification and ...
Populus tremula x Populus alba
Proc. Natl. Acad. Sci. USA
111
845-850
2014
-
1
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1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
743848
Ghosh
Characterization of developme ...
Hibiscus cannabinus
ScientificWorldJournal
2014
601845
2014
-
-
1
-
-
-
-
-
-
-
-
4
-
6
-
-
-
-
-
6
-
-
4
2
-
-
-
-
-
-
-
1
-
1
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
7
-
-
4
2
-
-
-
-
-
-
-
1
2
2
3
3
-
-
724494
Sonawane
In silico mutagenesis and dock ...
Leucaena leucocephala
Bioinformation
9
224-232
2013
-
-
-
-
33
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
33
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725147
Sonawane
Biochemical characterization o ...
Leucaena leucocephala
Int. J. Biol. Macromol.
58
154-159
2013
2
-
1
-
-
-
11
8
-
-
1
6
-
3
-
-
1
-
-
-
-
-
6
1
1
-
1
8
2
-
1
2
-
-
-
2
-
1
2
-
-
-
-
11
-
8
-
-
1
6
-
-
-
1
-
-
-
-
6
1
1
-
1
8
2
-
1
-
-
-
-
-
8
8
725148
Sonawane
Probing the active site of cin ...
Leucaena leucocephala
Int. J. Biol. Macromol.
60C
33-38
2013
-
-
1
-
14
-
7
12
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
14
-
-
7
-
12
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
12
-
-
-
-
-
-
-
-
12
12
743012
Khraiwesh
-
Molecular cloning and charact ...
Vaccinium corymbosum
J. Gen. Eng. Biotechnol.
11
1-8
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726155
Prashant
-
Isolation of cinnamoyl CoA red ...
Leucaena leucocephala
Plant Cell
108
421-436
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726178
Wang
-
Downregulation of cinnamoyl Co ...
Salvia miltiorrhiza
Plant Mol. Biol. Rep.
30
1229-1236
2012
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
725626
Tamasloukht
Characterization of a cinnamoy ...
Zea mays
J. Exp. Bot.
62
3837-3848
2011
-
-
1
-
-
-
-
3
-
-
-
3
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725759
Prasad
Structural and docking studies ...
Leucaena leucocephala
J. Mol. Model.
17
533-541
2011
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712994
Hu
Isolation and characterization ...
Isatis tinctoria
Mol. Biol. Rep.
38
2075-2083
2010
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726367
Zhou
Distinct cinnamoyl CoA reducta ...
Medicago truncatula
Proc. Natl. Acad. Sci. USA
107
17803-17808
2010
-
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
700489
Escamilla-Trevino
Switchgrass (Panicum virgatum) ...
Panicum virgatum
New Phytol.
185
143-155
2009
-
-
1
-
-
-
2
8
-
-
2
12
-
5
-
-
1
-
-
5
-
-
12
-
1
-
-
8
1
-
-
-
-
2
-
-
-
2
-
-
-
-
-
4
-
8
-
-
2
12
-
-
-
2
-
10
-
-
12
-
2
-
-
8
2
-
-
2
-
-
-
-
-
-
694669
Ralph
Identification of the structur ...
Arabidopsis thaliana, Nicotiana tabacum, Populus trichocarpa
Plant J.
53
368-379
2008
-
3
-
-
3
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694775
Mir Derikvand
Redirection of the phenylpropa ...
Arabidopsis thaliana
Planta
227
943-956
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695222
Wadenbaeck
Lignin biosynthesis in transge ...
Picea abies
Transgenic Res.
17
379-392
2008
-
1
1
-
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693407
Ma
Characterization of a cinnamoy ...
Triticum aestivum
J. Exp. Bot.
58
2011-2021
2007
1
1
1
-
-
-
1
4
-
-
1
-
-
2
-
-
1
-
-
4
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
4
-
-
1
-
-
-
-
1
-
4
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694626
Leple
Downregulation of cinnamoyl-co ...
Populus trichocarpa
Plant Cell
19
3669-3691
2007
-
1
1
-
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675132
van der Rest
Down-regulation of cinnamoyl-C ...
Solanum lycopersicum
J. Exp. Bot.
57
1399-1411
2006
-
-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
1
-
-
3
-
-
10
-
-
-
-
5
1
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
2
-
6
-
-
10
-
-
-
-
5
2
-
-
-
-
-
-
-
-
-
676522
Laskar
The Arabidopsis cinnamoyl CoA ...
Arabidopsis thaliana
Plant J.
48
674-686
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676843
Kawasaki
Cinnamoyl-CoA reductase, a key ...
Oryza sativa
Proc. Natl. Acad. Sci. USA
103
230-235
2006
2
-
1
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
672472
Ma
Biochemical characterization o ...
Triticum aestivum
Biol. Chem.
386
553-560
2005
-
-
1
-
-
-
-
5
-
-
2
1
-
3
-
-
1
-
-
3
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
2
1
-
-
-
1
-
3
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673861
Thumma
Polymorphisms in cinnamoyl CoA ...
Eucalyptus sp.
Genetics
171
1257-1265
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676451
Li
Clarification of cinnamoyl co- ...
Populus tremuloides
Plant Cell Physiol.
46
1073-1082
2005
-
-
1
-
-
-
4
4
-
-
-
1
-
1
-
-
1
-
-
1
5
-
6
-
-
-
-
4
1
1
-
1
6
-
-
-
-
1
1
-
-
-
-
4
6
4
-
-
-
1
-
-
-
1
-
1
5
-
6
-
-
-
-
4
1
1
-
-
-
-
-
-
-
-
676643
Baltas
Kinetic and inhibition studies ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
746-753
2005
-
-
1
-
-
-
5
3
-
-
1
1
-
3
-
-
1
-
-
-
-
-
4
-
1
-
-
3
1
-
-
1
10
-
-
-
-
1
1
-
-
-
-
5
10
3
-
-
1
1
-
-
-
1
-
-
-
-
4
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
656624
Larsen
Molecular cloning and characte ...
Hordeum vulgare, Solanum tuberosum
J. Plant Physiol.
161
105-112
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657109
Larsen
Cloning and characterization o ...
Lolium perenne
Plant Sci.
166
569-581
2004
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656926
Ruelland
-
Cellular and subcellular local ...
Saccharum officinarum, Zea mays
Physiol. Plant.
117
93-99
2003
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288238
Pincon
Simultaneous down-regulation o ...
Nicotiana tabacum
Plant Physiol.
126
145-155
2001
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288239
Lauvergeat
Two cinnamoyl-CoA reductase (C ...
Arabidopsis thaliana
Phytochemistry
57
1187-1195
2001
-
-
1
-
-
-
-
6
-
-
2
-
-
8
-
-
1
-
-
3
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
2
-
-
-
-
1
-
3
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
288237
Ralph
NMR characterization of altere ...
Nicotiana tabacum
Proc. Natl. Acad. Sci. USA
95
12803-12808
1998
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
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288240
Pichon
Cloning and characterization o ...
Zea mays
Plant Mol. Biol.
38
671-676
1998
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288235
Goffner
-
Purification and characterizat ...
Eucalyptus gunnii
Colloq. Inst. Natl. Rech. Agron.
69
329-330
1995
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4
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288236
Goffner
-
Cinnamoyl-CoA reductase and ci ...
Eucalyptus gunnii
Colloq. Inst. Natl. Rech. Agron.
69
331-332
1995
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288231
Grima-Pettenati
-
Stimulation of lignification i ...
Vigna mungo
Plant Sci.
61
179-188
1989
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2
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288232
Rolfs
Cell-suspension culture of Ara ...
Arachis hypogaea
Planta
172
238-244
1987
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4
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288228
Sarni
Purification and properties of ...
Populus x canadensis
Eur. J. Biochem.
139
259-265
1984
3
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3
6
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6
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1
1
4
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1
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288233
Luderitz
Enzymic synthesis of lignin pr ...
Glycine max, Picea abies
Eur. J. Biochem.
119
115-124
1981
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4
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1
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3
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5
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3
2
1
8
2
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2
1
8
2
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288230
Wengenmayer
Enzymic synthesis of lignin pr ...
Glycine max
Eur. J. Biochem.
65
529-536
1976
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4
9
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9
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6
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1
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288234
Gross
Reduction of coenzyme A thioes ...
Forsythia suspensa
FEBS Lett.
54
259-262
1975
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2
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8
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8
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389601
Rhodes
-
Enzymes involved in the reduct ...
Brassica napus subsp. rapifera
Phytochemistry
14
1235-1240
1975
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