BRENDA - Enzyme Database show
show all sequences of 1.2.1.44

Lignin biosynthesis in transgenic Norway spruce plants harboring an antisense construct for cinnamoyl CoA reductase (CCR)

Wadenbaeck, J.; von Arnold, S.; Egertsdotter, U.; Walter, M.H.; Grima-Pettenati, J.; Goffner, D.; Gellerstedt, G.; Gullion, T.; Clapham, D.; Transgenic Res. 17, 379-392 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
industry
down-regulated CCR lines with a reduced kappa number of small-scale Kraft pulps may be a starting point for constructing double mutants with enhanced properties of significance for practical forestry
Picea abies
Cloned(Commentary)
Commentary
Organism
five-year-old transgenic plants of Norway spruce Picea abies expressing the Norway spruce gene encoding CCR in antisense orientation under the control of the maize ubiquitin promoter
Picea abies
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
the transcript abundance of CCR can be reduced by 35% in transgenic Norway spruce plants expressing antisense CCR. Down-regulation of CCR results in up to 8% lower lignin content and the apparent contribution of H-lignin to the total lignin decreases. The kappa number of small-scale Kraft pulps is reduced in one CCR down-regulated line
Picea abies
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
cambial sap
Picea abies
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Picea abies
Q1EL79
-
-
Application (protein specific)
Application
Commentary
Organism
industry
down-regulated CCR lines with a reduced kappa number of small-scale Kraft pulps may be a starting point for constructing double mutants with enhanced properties of significance for practical forestry
Picea abies
Cloned(Commentary) (protein specific)
Commentary
Organism
five-year-old transgenic plants of Norway spruce Picea abies expressing the Norway spruce gene encoding CCR in antisense orientation under the control of the maize ubiquitin promoter
Picea abies
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
the transcript abundance of CCR can be reduced by 35% in transgenic Norway spruce plants expressing antisense CCR. Down-regulation of CCR results in up to 8% lower lignin content and the apparent contribution of H-lignin to the total lignin decreases. The kappa number of small-scale Kraft pulps is reduced in one CCR down-regulated line
Picea abies
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
cambial sap
Picea abies
-
-
Other publictions for EC 1.2.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724494
Sonawane
In silico mutagenesis and dock ...
Leucaena leucocephala
Bioinformation
9
224-232
2013
-
-
-
-
33
-
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6
-
2
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6
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2
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2
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33
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6
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6
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1
1
-
-
-
725147
Sonawane
Biochemical characterization o ...
Leucaena leucocephala
Int. J. Biol. Macromol.
58
154-159
2013
2
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1
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11
8
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1
6
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1
1
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8
2
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2
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2
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1
2
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11
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8
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1
6
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1
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6
1
1
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1
8
2
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1
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8
8
725148
Sonawane
Probing the active site of cin ...
Leucaena leucocephala
Int. J. Biol. Macromol.
60C
33-38
2013
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1
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14
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7
12
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2
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12
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12
12
726155
Prashant
-
Isolation of cinnamoyl CoA red ...
Leucaena leucocephala
Plant Cell
108
421-436
2012
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1
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726178
Wang
-
Downregulation of cinnamoyl Co ...
Salvia miltiorrhiza
Plant Mol. Biol. Rep.
30
1229-1236
2012
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1
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1
1
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1
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725626
Tamasloukht
Characterization of a cinnamoy ...
Zea mays
J. Exp. Bot.
62
3837-3848
2011
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1
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3
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3
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3
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1
1
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725759
Prasad
Structural and docking studies ...
Leucaena leucocephala
J. Mol. Model.
17
533-541
2011
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4
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4
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712994
Hu
Isolation and characterization ...
Isatis tinctoria
Mol. Biol. Rep.
38
2075-2083
2010
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1
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726367
Zhou
Distinct cinnamoyl CoA reducta ...
Medicago truncatula
Proc. Natl. Acad. Sci. USA
107
17803-17808
2010
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1
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5
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1
1
1
1
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700489
Escamilla-Trevino
Switchgrass (Panicum virgatum) ...
Panicum virgatum
New Phytol.
185
143-155
2009
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1
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2
8
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2
12
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5
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1
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5
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12
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1
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8
1
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2
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2
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4
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8
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2
12
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2
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10
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12
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2
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8
2
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2
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694669
Ralph
Identification of the structur ...
Arabidopsis thaliana, Nicotiana tabacum, Populus trichocarpa
Plant J.
53
368-379
2008
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3
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3
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7
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1
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694775
Mir Derikvand
Redirection of the phenylpropa ...
Arabidopsis thaliana
Planta
227
943-956
2008
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1
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695222
Wadenbaeck
Lignin biosynthesis in transge ...
Picea abies
Transgenic Res.
17
379-392
2008
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1
1
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1
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1
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3
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693407
Ma
Characterization of a cinnamoy ...
Triticum aestivum
J. Exp. Bot.
58
2011-2021
2007
1
1
1
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1
4
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1
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2
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4
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1
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1
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694626
Leple
Downregulation of cinnamoyl-co ...
Populus trichocarpa
Plant Cell
19
3669-3691
2007
-
1
1
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1
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1
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3
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1
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675132
van der Rest
Down-regulation of cinnamoyl-C ...
Solanum lycopersicum
J. Exp. Bot.
57
1399-1411
2006
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1
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1
2
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6
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1
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3
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10
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5
1
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1
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2
2
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2
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2
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6
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10
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5
2
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676522
Laskar
The Arabidopsis cinnamoyl CoA ...
Arabidopsis thaliana
Plant J.
48
674-686
2006
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676843
Kawasaki
Cinnamoyl-CoA reductase, a key ...
Oryza sativa
Proc. Natl. Acad. Sci. USA
103
230-235
2006
2
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1
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672472
Ma
Biochemical characterization o ...
Triticum aestivum
Biol. Chem.
386
553-560
2005
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1
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5
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1
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6
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673861
Thumma
Polymorphisms in cinnamoyl CoA ...
Eucalyptus sp.
Genetics
171
1257-1265
2005
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676451
Li
Clarification of cinnamoyl co- ...
Populus tremuloides
Plant Cell Physiol.
46
1073-1082
2005
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1
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4
4
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1
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5
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1
1
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6
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1
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6
4
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5
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6
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4
1
1
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676643
Baltas
Kinetic and inhibition studies ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
746-753
2005
-
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1
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5
3
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1
1
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3
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1
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4
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1
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3
1
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1
10
-
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1
1
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5
10
3
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1
1
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1
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4
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1
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3
1
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656624
Larsen
Molecular cloning and characte ...
Hordeum vulgare, Solanum tuberosum
J. Plant Physiol.
161
105-112
2004
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657109
Larsen
Cloning and characterization o ...
Lolium perenne
Plant Sci.
166
569-581
2004
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1
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1
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1
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1
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1
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1
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656926
Ruelland
-
Cellular and subcellular local ...
Saccharum officinarum, Zea mays
Physiol. Plant.
117
93-99
2003
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2
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2
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4
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288238
Pincon
Simultaneous down-regulation o ...
Nicotiana tabacum
Plant Physiol.
126
145-155
2001
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288239
Lauvergeat
Two cinnamoyl-CoA reductase (C ...
Arabidopsis thaliana
Phytochemistry
57
1187-1195
2001
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1
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6
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288237
Ralph
NMR characterization of altere ...
Nicotiana tabacum
Proc. Natl. Acad. Sci. USA
95
12803-12808
1998
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288240
Pichon
Cloning and characterization o ...
Zea mays
Plant Mol. Biol.
38
671-676
1998
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288235
Goffner
-
Purification and characterizat ...
Eucalyptus gunnii
Colloq. Inst. Natl. Rech. Agron.
69
329-330
1995
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288236
Goffner
-
Cinnamoyl-CoA reductase and ci ...
Eucalyptus gunnii
Colloq. Inst. Natl. Rech. Agron.
69
331-332
1995
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288231
Grima-Pettenati
-
Stimulation of lignification i ...
Vigna mungo
Plant Sci.
61
179-188
1989
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288232
Rolfs
Cell-suspension culture of Ara ...
Arachis hypogaea
Planta
172
238-244
1987
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4
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288228
Sarni
Purification and properties of ...
Populus x canadensis
Eur. J. Biochem.
139
259-265
1984
3
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6
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288233
Luderitz
Enzymic synthesis of lignin pr ...
Glycine max, Picea abies
Eur. J. Biochem.
119
115-124
1981
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4
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288230
Wengenmayer
Enzymic synthesis of lignin pr ...
Glycine max
Eur. J. Biochem.
65
529-536
1976
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4
9
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288234
Gross
Reduction of coenzyme A thioes ...
Forsythia suspensa
FEBS Lett.
54
259-262
1975
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2
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389601
Rhodes
-
Enzymes involved in the reduct ...
Brassica napus subsp. rapifera
Phytochemistry
14
1235-1240
1975
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