Cloned (Comment) | Organism |
---|---|
into the pGEX-4T vector for expression in Escherichia coli BL21DE3 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate | - |
Arabidopsis thaliana | |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate | - |
Arabidopsis thaliana | |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate | - |
Arabidopsis thaliana | |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate | - |
Arabidopsis thaliana | |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
feruloyl-CoA | - |
Arabidopsis thaliana | |
0.00055 | - |
sinapoyl-CoA | - |
Arabidopsis thaliana | |
0.00227 | - |
p-Coumaroyl-CoA | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
determined by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + CoA + NADP+ | Arabidopsis thaliana | - |
cinnamoyl-CoA + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a glutathione-sepharose 4 Fast Flow column, purified GST-AtCCR1 is cleaved by thrombin to remove the GST tag | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + CoA + NADP+ | - |
Arabidopsis thaliana | cinnamoyl-CoA + NADPH + H+ | - |
? | |
feruloyl-CoA + NADPH + H+ | - |
Arabidopsis thaliana | coniferaldehyde + CoA + NADP+ | - |
? | |
p-coumaroyl-CoA + NADPH + H+ | - |
Arabidopsis thaliana | p-coumaraldehyde + CoA + NADP+ | - |
? | |
sinapoyl-CoA + NADPH + H+ | - |
Arabidopsis thaliana | sinapaldehyde + CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtCCR1 | - |
Arabidopsis thaliana |
CCR | ambiguous | Arabidopsis thaliana |
cinnamoyl-CoA reductase | - |
Arabidopsis thaliana |
cinnamoyl-CoA reductase 1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.63 | - |
p-Coumaroyl-CoA | - |
Arabidopsis thaliana | |
1.67 | - |
sinapoyl-CoA | - |
Arabidopsis thaliana | |
1.9 | - |
feruloyl-CoA | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.25 | - |
activity assay | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate | with feruloyl-CoA as substrate, noncompetitive inhibition | Arabidopsis thaliana | |
0.0071 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate | with sinapoyl-CoA as substrate, noncompetitive inhibition | Arabidopsis thaliana | |
0.031 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate | with feruloyl-CoA as substrate, uncompetitive inhibition | Arabidopsis thaliana | |
0.055 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate | with feruloyl-CoA as substrate, uncompetitive inhibition | Arabidopsis thaliana | |
0.057 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate | with sinapoyl-CoA as substrate, noncompetitive inhibition | Arabidopsis thaliana | |
0.068 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate | with sinapoyl-CoA as substrate, noncompetitive inhibition | Arabidopsis thaliana | |
0.156 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate | with sinapoyl-CoA as substrate, uncompetitive inhibition | Arabidopsis thaliana | |
0.212 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate | with sinapoyl-CoA as substrate, uncompetitive inhibition | Arabidopsis thaliana | |
0.29 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate | with feruloyl-CoA as substrate, uncompetitive inhibition | Arabidopsis thaliana | |
0.422 | - |
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate | with feruloyl-CoA as substrate, uncompetitive inhibition | Arabidopsis thaliana |