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Literature summary for 1.2.1.41 extracted from
- Proline biosynthesis in Escherichia coli. Purification and characterisation of glutamate-semialdehyde dehydrogenase (1982), Eur. J. Biochem., 121, 561-565.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 50% inhibition at 1 mM | Escherichia coli |  |
| Co2+ | 50% inhibition at 2.5 mM | Escherichia coli | |
| Cu2+ | 75% inhibition at 0.1 mM | Escherichia coli | |
| iodoacetamide | 40% inhibition at 1 mM | Escherichia coli | |
| iodoacetate | 15% inhibition at 1 mM | Escherichia coli | |
| L-proline | 25% inhibition at 50 mM | Pseudomonas aeruginosa | |
| Ni2+ | 100% inhibition at 0.1 mM | Escherichia coli | |
| p-chloromercuribenzoate | 100% inhibition at 1 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
SDS-PAGE, monomeric form | Escherichia coli |
| 189000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.84 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months | Escherichia coli |
| -70°C, 10% glycerol | Escherichia coli |
| 0-2°C, 2 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate 5-semialdehyde + phosphate + NADP+ | - |
Escherichia coli | L-5-glutamyl phosphate + NADPH | - |
? | |
| L-glutamate 5-semialdehyde + phosphate + NADP+ | - |
Pseudomonas aeruginosa | L-5-glutamyl phosphate + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 47000, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
rapidly inactivated above, pH 7.0, 30 min, stable below | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0 and pH 8.5: about 20% of activity maximum | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | stable for at least 10 min | Escherichia coli |
| 7 | - |
long periods | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
| NADPH | - |
Pseudomonas aeruginosa | |
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Release 2023.1 (January 2023)
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