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Literature summary for 1.2.1.33 extracted from

  • Magee, P.T.; Snell, E.E.
    The bacterial degradation of pantothenic acid. IV. Enzymatic conversion of aldopantoate to alpha-ketoisovalerate (1966), Biochemistry, 5, 409-416.
    View publication on PubMed
Search for more literature for 1.2.1.33

General Stability

General Stability Organism
aldopantoate stabilizes against heat Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
(R)-4-dehydropantoate
-
 Pseudomonas sp.
NAD+ above 0.3 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NAD+
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-4-dehydropantoate + NAD+ Pseudomonas sp. enzyme required for degradation of pantothenate to alpha-ketoisovalerate (R)-3,3-dimethylmalate + NADH
-
 ?
(R)-4-dehydropantoate + NAD+ Pseudomonas sp. P-2 enzyme required for degradation of pantothenate to alpha-ketoisovalerate (R)-3,3-dimethylmalate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 P-2
-
Pseudomonas sp. P-2
-
 P-2
-

Purification (Commentary)

Purification (Comment) Organism
P-2, partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
0°C, 0.1 M potassium phosphate pH 7.5, 0.015 M mercaptoethanol, 3 days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-4-dehydropantoate + NAD+ enzyme required for degradation of pantothenate to alpha-ketoisovalerate Pseudomonas sp. (R)-3,3-dimethylmalate + NADH
-
 ?
(R)-4-dehydropantoate + NAD+ enzyme required for degradation of pantothenate to alpha-ketoisovalerate Pseudomonas sp. P-2 (R)-3,3-dimethylmalate + NADH
-
 ?
(R)-4-dehydropantoate + NAD+ + H2O
-
 Pseudomonas sp. (R)-3,3-dimethylmalate + NADH
-
 ?
(R)-4-dehydropantoate + NAD+ + H2O
-
 Pseudomonas sp. P-2 (R)-3,3-dimethylmalate + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 4 min in presence of aldopantoate: no loss of activity, 1 min in absence of aldopantoate, complete loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 or higher, activity increases from pH 7.0 to 9.5 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 activity increases from pH 7.0 to 9.5 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information no diffusible cofactors found Pseudomonas sp.