Literature summary for 1.2.1.30 extracted from

Finnigan, W.; Thomas, A.; Cromar, H.; Gough, B.; Snajdrova, R.; Adams, J.P.; Littlechild, J.A.; Harmer, N.J.
Characterization of carboxylic acid reductases as enzymes in the toolbox for synthetic chemistry (2017), ChemCatChem, 9, 1005-1017 .

Application

Application	Comment	Organism
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts	Neurospora crassa
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts	Mycolicibacterium phlei
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts	Nocardia asteroides
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts, application for the reduction of fatty acids to fatty alcohols	Mycolicibacterium smegmatis
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts, application for the reduction of fatty acids to fatty alcohols	Trametes versicolor
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts, application for the reduction of fatty acids to fatty alcohols	Syncephalastrum racemosum
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts, application for the reduction of fatty acids to fatty alcohols	Nocardia otitidiscaviarum
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts, application for the reduction of fatty acids to fatty alcohols	Tsukamurella paurometabola
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts, application for the reduction of fatty acids to fatty alcohols	Nocardia brasiliensis
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts, application for the reduction of fatty acids to fatty alcohols	Mycobacterium marinum
synthesis	carboxylic acid reductases are important enzymes in the toolbox for sustainable chemistry and provide specific use as biocatalysts. The reduction of racemic ibuprofen by whole Nocardia iowensis cells gives an enantiomeric excess (ee) of 61.2%, which is attributed to enantioselectivity by niCAR based on kinetic data for its reduction of (S)-(+)- and (R)-(-)-ibuprofen enantiomers	Nocardia iowensis

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Mycolicibacterium smegmatis
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Neurospora crassa
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Mycolicibacterium phlei
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Nocardia asteroides
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Trametes versicolor
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Syncephalastrum racemosum
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Nocardia otitidiscaviarum
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Tsukamurella paurometabola
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Nocardia iowensis
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Nocardia brasiliensis
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Mycobacterium marinum

Inhibitors

Inhibitors	Comment	Organism
AMP	product inhibition	Mycobacterium marinum
AMP	product inhibition, competitive versus ATP	Mycolicibacterium phlei
AMP	product inhibition	Mycolicibacterium smegmatis
AMP	product inhibition	Neurospora crassa
AMP	product inhibition	Nocardia asteroides
AMP	product inhibition	Nocardia brasiliensis
AMP	product inhibition	Nocardia iowensis
AMP	product inhibition	Nocardia otitidiscaviarum
AMP	product inhibition	Syncephalastrum racemosum
AMP	product inhibition	Trametes versicolor
AMP	product inhibition	Tsukamurella paurometabola
diphosphate	product inhibition	Mycobacterium marinum
diphosphate	product inhibition, mixed inhibition with ATP, competitive versus 4-methylbenzoic acid	Mycolicibacterium phlei
diphosphate	product inhibition	Mycolicibacterium smegmatis
diphosphate	product inhibition	Neurospora crassa
diphosphate	product inhibition	Nocardia asteroides
diphosphate	product inhibition	Nocardia brasiliensis
diphosphate	product inhibition	Nocardia iowensis
diphosphate	product inhibition	Nocardia otitidiscaviarum
diphosphate	product inhibition	Syncephalastrum racemosum
diphosphate	product inhibition	Trametes versicolor
diphosphate	product inhibition	Tsukamurella paurometabola
NADP+	product inhibition	Mycobacterium marinum
NADP+	product inhibition, NADP+ shows competitive inhibition with NADPH	Mycolicibacterium phlei
NADP+	product inhibition	Mycolicibacterium smegmatis
NADP+	product inhibition	Neurospora crassa
NADP+	product inhibition	Nocardia asteroides
NADP+	product inhibition	Nocardia brasiliensis
NADP+	product inhibition	Nocardia iowensis
NADP+	product inhibition	Nocardia otitidiscaviarum
NADP+	product inhibition	Syncephalastrum racemosum
NADP+	product inhibition	Trametes versicolor
NADP+	product inhibition	Tsukamurella paurometabola

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	kinetic analysis	Mycolicibacterium smegmatis
additional information	-	additional information	kinetic analysis	Mycolicibacterium phlei
additional information	-	additional information	kinetic analysis	Nocardia otitidiscaviarum
additional information	-	additional information	kinetic analysis	Tsukamurella paurometabola
additional information	-	additional information	kinetic analysis	Nocardia iowensis
0.006	-	trans-2-phenylcyclopropane-1-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.02	-	dodecanoate	pH 7.5, 30°C	Nocardia iowensis
0.02	-	octadecanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.02	-	octadecanoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.04	-	dodecanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.04	-	dodecanoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.05	-	(E)-3-phenylprop-2-enoate	pH 7.5, 30°C	Nocardia iowensis
0.05	-	dodecanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.061	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
0.075	-	(E)-3-phenylprop-2-enoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.09	-	3-phenylprop-2-ynoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.09	-	dodecanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.09	-	octadecanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.1	-	Octanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.16	-	3-Phenylpropionate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.16	-	4-Methylbenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
0.19	-	4-Methoxybenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
0.2	-	Octanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.2	-	Octanoate	pH 7.5, 30°C	Nocardia iowensis
0.2	-	Octanoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.21	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.5, 30°C	Nocardia iowensis
0.25	-	4-Methoxybenzoate	pH 7.5, 30°C	Nocardia iowensis
0.27	-	3-oxo-3-phenylpropanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.29	-	3-oxo-3-phenylpropanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.3	-	3-Nitrobenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.3	-	(E)-3-phenylprop-2-enoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.31	-	(E)-3-phenylprop-2-enoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.32	-	3-Phenylpropionate	pH 7.8, 30°C	Tsukamurella paurometabola
0.39	-	3-oxo-3-phenylpropanoate	pH 7.5, 30°C	Nocardia iowensis
0.45	-	4-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.5	-	3-Nitrobenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
0.55	-	3-oxo-3-phenylpropanoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.56	-	3-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.6	-	octadecanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.6	-	4-nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.68	-	3-Methoxybenzoate	pH 7.5, 30°C	Nocardia iowensis
0.69	-	4-Methylbenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.7	-	octadecanoate	pH 7.5, 30°C	Nocardia iowensis
0.7	-	3-Nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.72	-	(E)-3-phenylprop-2-enoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.9	-	benzoate	pH 7.5, 30°C	Nocardia iowensis
0.9	-	3-Methoxybenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.97	-	3-Phenylpropionate	pH 7.5, 30°C	Nocardia iowensis
1	-	4-Methylbenzoate	pH 7.5, 30°C	Nocardia iowensis
1	-	Thiophene-2-carboxylate	pH 7.5, 30°C	Nocardia iowensis
1	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.1	-	4-Methoxybenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.2	-	4-Methylbenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.3	-	3-phenylprop-2-ynoate	pH 7.5, 30°C	Nocardia iowensis
1.8	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.3, 30°C	Mycolicibacterium phlei
2	-	benzoate	pH 7.8, 30°C	Tsukamurella paurometabola
2	-	Octanoate	pH 7.3, 30°C	Mycolicibacterium phlei
2.1	-	benzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.5	-	3-Nitrobenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.6	-	Thiophene-2-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.7	-	3-Phenylpropionate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.8	-	4-Methoxybenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
3	-	3-Methoxybenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
3	-	3-Phenylpropionate	pH 7.3, 30°C	Mycolicibacterium phlei
3.3	-	Thiophene-2-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
3.3	-	Thiophene-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
3.4	-	benzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
3.7	-	4-Methylbenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
3.8	-	3-oxo-3-phenylpropanoate	pH 7.3, 30°C	Mycolicibacterium phlei
4.7	-	furan-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
5	-	Butanoate	pH 7.8, 30°C	Tsukamurella paurometabola
5.6	-	3-Nitrobenzoate	pH 7.5, 30°C	Nocardia iowensis
7.9	-	Butanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
9	-	2-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
12	-	3-Methoxybenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
13	-	furan-2-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
20	-	pyridine-2-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
20	-	benzoate	pH 7.3, 30°C	Mycolicibacterium phlei
24	-	pyridine-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
32	-	Butanoate	pH 7.5, 30°C	Nocardia iowensis
50	-	Butanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
50	-	Thiophene-2-carboxylate	pH 7.3, 30°C	Mycolicibacterium phlei

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium marinum	B2HN69	-	-
Mycobacterium marinum ATCC BAA-535	B2HN69	-	-
Mycolicibacterium phlei	-	-	-
Mycolicibacterium smegmatis	-	-	-
Neurospora crassa	-	-	-
Nocardia asteroides	-	-	-
Nocardia asteroides JCM 3016	-	-	-
Nocardia brasiliensis	-	-	-
Nocardia iowensis	Q6RKB1	-	-
Nocardia otitidiscaviarum	-	-	-
Syncephalastrum racemosum	-	-	-
Trametes versicolor	-	-	-
Tsukamurella paurometabola	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Mycolicibacterium smegmatis	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Neurospora crassa	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Nocardia asteroides	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Trametes versicolor	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Syncephalastrum racemosum	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Nocardia otitidiscaviarum	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Tsukamurella paurometabola	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Nocardia iowensis	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Nocardia brasiliensis	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Mycobacterium marinum	a
an aromatic aldehyde + NADP+ + AMP + diphosphate = an aromatic acid + NADPH + H+ + ATP	product inhibition by NADP+, adenosine monophosphate, and diphosphate indicates that the binding of substrates at the adenylation domain is ordered with ATP binding first, proposed catalytic mechanism in 4 steps, overview. The first two steps, the relatively unreactive carboxylic acid is activated to form a thioester with the phosphopantetheine arm at the N-terminal adenylation domain (1) ATP and a carboxylic acid enter the active site of the adenylation domain in which the alpha-phosphate of ATP is attacked by an O-atom from the carboxylic acid to form an AMP-acyl phosphoester with the release of diphosphate.(2) The thiol group of the phosphopantetheine arm can then attack the carbonyl carbon atom of the AMP-acyl phosphoester intermediate nucleophilically to release AMP and to form an acyl thioester with the phosphopantetheine arm. (3) The phosphopantetheine arm transfers to the C-terminal reductase domain in which (4) the thioester is reduced by NADPH, the aldehyde and NADP+ are released, and the thiol of the phosphopantetheine arm is regenerated in the process	Mycolicibacterium phlei	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(E)-3-phenylprop-2-enoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	(E)-3-phenylprop-2-enal + NADP+ + AMP + diphosphate	-	?
(E)-3-phenylprop-2-enoate + NADPH + H+ + ATP	-	Nocardia iowensis	(E)-3-phenylprop-2-enal + NADP+ + AMP + diphosphate	-	?
(E)-3-phenylprop-2-enoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	(E)-3-phenylprop2-enal + NADP+ + AMP + diphosphate	-	?
(E)-3-phenylprop-2-enoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	(E)-3-phenylprop2-enal + NADP+ + AMP + diphosphate	-	?
(E)-3-phenylprop-2-enoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	(E)-3-phenylprop2-enal + NADP+ + AMP + diphosphate	-	?
2-methoxybenzoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	2-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-methoxybenzoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	3-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-methoxybenzoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	3-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-methoxybenzoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	3-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-methoxybenzoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	3-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-methoxybenzoate + NADPH + H+ + ATP	-	Nocardia iowensis	3-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-nitrobenzoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	3-nitrobenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-nitrobenzoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	3-nitrobenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-nitrobenzoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	3-nitrobenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-nitrobenzoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	3-nitrobenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-nitrobenzoate + NADPH + H+ + ATP	-	Nocardia iowensis	3-nitrobenzaldehyde + NADP+ + AMP + diphosphate	-	?
3-oxo-3-phenylpropanoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	3-oxo-3-phenylpropanal + NADP+ + AMP + diphosphate	-	?
3-oxo-3-phenylpropanoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	3-oxo-3-phenylpropanal + NADP+ + AMP + diphosphate	-	?
3-oxo-3-phenylpropanoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	3-oxo-3-phenylpropanal + NADP+ + AMP + diphosphate	-	?
3-oxo-3-phenylpropanoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	3-oxo-3-phenylpropanal + NADP+ + AMP + diphosphate	-	?
3-oxo-3-phenylpropanoate + NADPH + H+ + ATP	-	Nocardia iowensis	3-oxo-3-phenylpropanal + NADP+ + AMP + diphosphate	-	?
3-phenylprop-2-ynoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	3-phenylprop-2-ynal + NADP+ + AMP + diphosphate	-	?
3-phenylprop-2-ynoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	3-phenylprop-2-ynal + NADP+ + AMP + diphosphate	-	?
3-phenylprop-2-ynoate + NADPH + H+ + ATP	-	Nocardia iowensis	3-phenylprop-2-ynal + NADP+ + AMP + diphosphate	-	?
3-phenylpropionate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	3-phenylpropionaldehyde + NADP+ + AMP + diphosphate	-	?
3-phenylpropionate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	3-phenylpropionaldehyde + NADP+ + AMP + diphosphate	-	?
3-phenylpropionate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	3-phenylpropionaldehyde + NADP+ + AMP + diphosphate	-	?
3-phenylpropionate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	3-phenylpropionaldehyde + NADP+ + AMP + diphosphate	-	?
3-phenylpropionate + NADPH + H+ + ATP	-	Nocardia iowensis	3-phenylpropionaldehyde + NADP+ + AMP + diphosphate	-	?
4-methoxybenzoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	4-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methoxybenzoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	4-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methoxybenzoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	4-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methoxybenzoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	4-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methoxybenzoate + NADPH + H+ + ATP	-	Nocardia iowensis	4-methoxybenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methylbenzoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	4-methylbenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methylbenzoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	4-methylbenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methylbenzoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	4-methylbenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methylbenzoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	4-methylbenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-methylbenzoate + NADPH + H+ + ATP	-	Nocardia iowensis	4-methylbenzaldehyde + NADP+ + AMP + diphosphate	-	?
4-nitrobenzoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	4-nitrobenzaldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Neurospora crassa	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Mycolicibacterium phlei	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Nocardia asteroides	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Trametes versicolor	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Syncephalastrum racemosum	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Tsukamurella paurometabola	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Nocardia iowensis	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Nocardia brasiliensis	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Mycobacterium marinum	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Mycobacterium marinum ATCC BAA-535	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
aromatic acid + NADPH + H+ + ATP	-	Nocardia asteroides JCM 3016	aromatic aldehyde + NADP+ + AMP + diphosphate	-	?
benzoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	benzaldehyde + NADP+ + AMP + diphosphate	-	?
benzoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	benzaldehyde + NADP+ + AMP + diphosphate	-	?
benzoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	benzaldehyde + NADP+ + AMP + diphosphate	-	?
benzoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	benzaldehyde + NADP+ + AMP + diphosphate	-	?
benzoate + NADPH + H+ + ATP	-	Nocardia iowensis	benzaldehyde + NADP+ + AMP + diphosphate	-	?
butanoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	butyraldehyde + NADP+ + AMP + diphosphate	-	?
butanoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	butyraldehyde + NADP+ + AMP + diphosphate	-	?
butanoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	butyraldehyde + NADP+ + AMP + diphosphate	-	?
butanoate + NADPH + H+ + ATP	-	Nocardia iowensis	butyraldehyde + NADP+ + AMP + diphosphate	-	?
dodecanoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	dodecanal + NADP+ + AMP + diphosphate	-	?
dodecanoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	dodecanal + NADP+ + AMP + diphosphate	-	?
dodecanoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	dodecanal + NADP+ + AMP + diphosphate	-	?
dodecanoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	dodecanal + NADP+ + AMP + diphosphate	-	?
dodecanoate + NADPH + H+ + ATP	-	Nocardia iowensis	dodecanal + NADP+ + AMP + diphosphate	-	?
furan-2-carboxylate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	furan-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
furan-2-carboxylate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	furan-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
additional information	enzyme CAR prefers substrates in which the carboxylic acid is the only polar or charged group, which gives a useful insight into the substrate specificity of the enzymes. Model development for the prediction of CAR reactivity	Nocardia brasiliensis	?	-	-
additional information	enzyme CAR prefers substrates in which the carboxylic acid is the only polar or charged group, which gives a useful insight into the substrate specificity of the enzymes. Model development for the prediction of CAR reactivity. No activity with 2-methoxybenzoate, 4-nitrobenzoate, 2-nitrobenzoate, phenylpropynoate, pyridine-2-carboxylate, and 1H-pyrrole-2-carboxylate	Mycolicibacterium smegmatis	?	-	-
additional information	no activity with 2-methoxybenzoate, 4-nitrobenzoate, 2-nitrobenzoate, 1H-pyrrole-2-carboxylate and furan-2-carboxylate	Nocardia otitidiscaviarum	?	-	-
additional information	no activity with 2-methoxybenzoate, 4-nitrobenzoate, 2-nitrobenzoate, phenylpropynoate, butanoate, pyridine-2-carboxylate, 1H-pyrrole-2-carboxylate, and furan-2-carboxylate	Mycolicibacterium phlei	?	-	-
additional information	no activity with 2-methoxybenzoate, 4-nitrobenzoate, 2-nitrobenzoate, pyridine-2-carboxylate, 1H-pyrrole-2-carboxylate, and furan-2-carboxylate	Nocardia iowensis	?	-	-
additional information	no activity with 2-nitrobenzoate and 1H-pyrrole-2-carboxylate	Tsukamurella paurometabola	?	-	-
additional information	the enzyme is active against C2-C18 fatty acids	Trametes versicolor	?	-	-
additional information	the enzyme is active against C2-C18 fatty acids	Syncephalastrum racemosum	?	-	-
additional information	the enzyme is active against C2-C18 fatty acids. Enzyme CAR prefers substrates in which the carboxylic acid is the only polar or charged group, which gives a useful insight into the substrate specificity of the enzymes. Model development for the prediction of CAR reactivity	Mycobacterium marinum	?	-	-
additional information	the enzyme prefers benzoates and aliphatic acids that are substituted with a phenyl group in the 3-position. No reaction of this CAR with simple aliphatic acids	Nocardia asteroides	?	-	-
additional information	the enzyme is active against C2-C18 fatty acids. Enzyme CAR prefers substrates in which the carboxylic acid is the only polar or charged group, which gives a useful insight into the substrate specificity of the enzymes. Model development for the prediction of CAR reactivity	Mycobacterium marinum ATCC BAA-535	?	-	-
additional information	the enzyme prefers benzoates and aliphatic acids that are substituted with a phenyl group in the 3-position. No reaction of this CAR with simple aliphatic acids	Nocardia asteroides JCM 3016	?	-	-
octadecanoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	octadecanal + NADP+ + AMP + diphosphate	-	?
octadecanoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	octadecanal + NADP+ + AMP + diphosphate	-	?
octadecanoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	octadecanal + NADP+ + AMP + diphosphate	-	?
octadecanoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	octadecanal + NADP+ + AMP + diphosphate	-	?
octadecanoate + NADPH + H+ + ATP	-	Nocardia iowensis	octadecanal + NADP+ + AMP + diphosphate	-	?
octanoate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	octanal + NADP+ + AMP + diphosphate	-	?
octanoate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	octanal + NADP+ + AMP + diphosphate	-	?
octanoate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	octanal + NADP+ + AMP + diphosphate	-	?
octanoate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	octanal + NADP+ + AMP + diphosphate	-	?
octanoate + NADPH + H+ + ATP	-	Nocardia iowensis	octanal + NADP+ + AMP + diphosphate	-	?
pyridine-2-carboxylate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	pyridine-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
pyridine-2-carboxylate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	pyridine-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
thiophene-2-carboxylate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	thiophene-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
thiophene-2-carboxylate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	thiophene-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
thiophene-2-carboxylate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	thiophene-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
thiophene-2-carboxylate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	thiophene-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
thiophene-2-carboxylate + NADPH + H+ + ATP	-	Nocardia iowensis	thiophene-2-carbaldehyde + NADP+ + AMP + diphosphate	-	?
trans-2-phenylcyclopropane-1-carboxylate + NADPH + H+ + ATP	-	Mycolicibacterium smegmatis	trans-2-phenylcyclopropane-1-carbaldehyde + NADP+ + AMP + diphosphate	-	?
trans-2-phenylcyclopropane-1-carboxylate + NADPH + H+ + ATP	-	Mycolicibacterium phlei	trans-2-phenylcyclopropane-1-carbaldehyde + NADP+ + AMP + diphosphate	-	?
trans-2-phenylcyclopropane-1-carboxylate + NADPH + H+ + ATP	-	Nocardia otitidiscaviarum	trans-2-phenylcyclopropane-1-carbaldehyde + NADP+ + AMP + diphosphate	-	?
trans-2-phenylcyclopropane-1-carboxylate + NADPH + H+ + ATP	-	Tsukamurella paurometabola	trans-2-phenylcyclopropane-1-carbaldehyde + NADP+ + AMP + diphosphate	-	?
trans-2-phenylcyclopropane-1-carboxylate + NADPH + H+ + ATP	-	Nocardia iowensis	trans-2-phenylcyclopropane-1-carbaldehyde + NADP+ + AMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
aryl aldehyde:NADP+ oxidoreductase	-	Neurospora crassa
ATP/NADPH-dependent carboxylic acid reductase	-	Mycobacterium marinum
CAR	-	Mycolicibacterium smegmatis
CAR	-	Neurospora crassa
CAR	-	Mycolicibacterium phlei
CAR	-	Nocardia asteroides
CAR	-	Trametes versicolor
CAR	-	Syncephalastrum racemosum
CAR	-	Nocardia otitidiscaviarum
CAR	-	Tsukamurella paurometabola
CAR	-	Nocardia iowensis
CAR	-	Nocardia brasiliensis
CAR	-	Mycobacterium marinum
Carboxylic acid reductase	-	Mycolicibacterium smegmatis
Carboxylic acid reductase	-	Neurospora crassa
Carboxylic acid reductase	-	Mycolicibacterium phlei
Carboxylic acid reductase	-	Nocardia asteroides
Carboxylic acid reductase	-	Trametes versicolor
Carboxylic acid reductase	-	Syncephalastrum racemosum
Carboxylic acid reductase	-	Nocardia otitidiscaviarum
Carboxylic acid reductase	-	Tsukamurella paurometabola
Carboxylic acid reductase	-	Nocardia iowensis
Carboxylic acid reductase	-	Nocardia brasiliensis
Carboxylic acid reductase	-	Mycobacterium marinum
mpCAR	-	Mycolicibacterium phlei
mpCAR	-	Nocardia asteroides
msCAR	-	Mycolicibacterium smegmatis
niCAR	-	Nocardia iowensis
noCAR	-	Nocardia otitidiscaviarum
tpCAR	-	Tsukamurella paurometabola

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Mycolicibacterium smegmatis
30	-	assay at	Mycolicibacterium phlei
30	-	assay at	Nocardia iowensis
31	-	-	Tsukamurella paurometabola
38	-	-	Nocardia otitidiscaviarum
40	-	-	Nocardia asteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	enzyme half-lives at different conditions, overview	Mycolicibacterium smegmatis
additional information	-	enzyme half-lives at different conditions, overview	Mycolicibacterium phlei
additional information	-	enzyme half-lives at different conditions, overview	Nocardia otitidiscaviarum
additional information	-	enzyme half-lives at different conditions, overview	Tsukamurella paurometabola
additional information	-	enzyme half-lives at different conditions, overview	Nocardia iowensis
30	-	half-life is 132.2 h	Mycolicibacterium phlei
30	-	half-life is 35.3 h	Nocardia otitidiscaviarum
30	-	half-life is 42.9 h	Nocardia iowensis
30	-	half-life is 53.7 h	Mycolicibacterium smegmatis
30	-	half-life of the enzyme is 25 h	Tsukamurella paurometabola
42	50	enzyme mpCAR retains 92% of its activity following the same incubation at 42°C. mpCAR is able to retain residual activity up to 50°C	Mycolicibacterium phlei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.062	-	3-Nitrobenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.062	-	octadecanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.1	-	3-phenylprop-2-ynoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.117	-	3-phenylprop-2-ynoate	pH 7.5, 30°C	Nocardia iowensis
0.128	-	(E)-3-phenylprop-2-enoate	pH 7.5, 30°C	Nocardia iowensis
0.18	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.5, 30°C	Nocardia iowensis
0.183	-	octadecanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.25	-	octadecanoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.3	-	3-Nitrobenzoate	pH 7.5, 30°C	Nocardia iowensis
0.3	-	3-Methoxybenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
0.3	-	3-oxo-3-phenylpropanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.317	-	2-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.32	-	furan-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
0.33	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.3, 30°C	Mycolicibacterium phlei
0.358	-	3-Phenylpropionate	pH 7.3, 30°C	Mycolicibacterium phlei
0.37	-	trans-2-phenylcyclopropane-1-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.38	-	pyridine-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
0.55	-	3-Nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.618	-	3-oxo-3-phenylpropanoate	pH 7.5, 30°C	Nocardia iowensis
0.63	-	(E)-3-phenylprop-2-enoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.67	-	3-Nitrobenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
0.717	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
0.77	-	octadecanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.8	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.82	-	4-Methoxybenzoate	pH 7.5, 30°C	Nocardia iowensis
0.83	-	furan-2-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.83	-	Thiophene-2-carboxylate	pH 7.3, 30°C	Mycolicibacterium phlei
0.92	-	dodecanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.97	-	Octanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.983	-	3-Nitrobenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.01	-	Thiophene-2-carboxylate	pH 7.5, 30°C	Nocardia iowensis
1.05	-	3-oxo-3-phenylpropanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.12	-	(E)-3-phenylprop-2-enoate	pH 7.3, 30°C	Mycolicibacterium phlei
1.13	-	octadecanoate	pH 7.5, 30°C	Nocardia iowensis
1.25	-	3-oxo-3-phenylpropanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
1.27	-	pyridine-2-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.37	-	Butanoate	pH 7.8, 30°C	Tsukamurella paurometabola
1.37	-	Thiophene-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
1.42	-	3-oxo-3-phenylpropanoate	pH 7.8, 30°C	Tsukamurella paurometabola
1.43	-	3-Phenylpropionate	pH 7.5, 30°C	Nocardia iowensis
1.55	-	3-Methoxybenzoate	pH 7.5, 30°C	Nocardia iowensis
1.57	-	4-Methylbenzoate	pH 7.5, 30°C	Nocardia iowensis
1.63	-	benzoate	pH 7.5, 30°C	Nocardia iowensis
1.65	-	dodecanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.73	-	3-Methoxybenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
1.75	-	(E)-3-phenylprop-2-enoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.97	-	(E)-3-phenylprop-2-enoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
2.03	-	4-Methylbenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
2.05	-	Thiophene-2-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
2.15	-	Butanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
2.17	-	4-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
2.17	-	4-nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
2.18	-	dodecanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
2.2	-	4-Methoxybenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
2.25	-	Thiophene-2-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.27	-	3-Methoxybenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.3	-	4-Methoxybenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.3	-	4-Methylbenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.33	-	3-Phenylpropionate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.33	-	benzoate	pH 7.3, 30°C	Mycolicibacterium phlei
2.35	-	Octanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.37	-	benzoate	pH 7.8, 30°C	Tsukamurella paurometabola
2.53	-	4-Methylbenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
2.57	-	4-Methylbenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
2.62	-	dodecanoate	pH 7.5, 30°C	Nocardia iowensis
2.62	-	dodecanoate	pH 7.8, 30°C	Tsukamurella paurometabola
2.63	-	3-Phenylpropionate	pH 7.8, 30°C	Tsukamurella paurometabola
2.83	-	Butanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.98	-	4-Methoxybenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
3.05	-	benzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
3.07	-	3-Phenylpropionate	pH 8.0, 30°C	Mycolicibacterium smegmatis
3.1	-	3-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
3.28	-	benzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
3.65	-	Octanoate	pH 7.8, 30°C	Tsukamurella paurometabola
3.88	-	Octanoate	pH 7.5, 30°C	Nocardia iowensis
4.33	-	Butanoate	pH 7.5, 30°C	Nocardia iowensis
4.93	-	Octanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	-	Mycolicibacterium phlei
7.5	-	-	Nocardia asteroides
7.5	-	-	Nocardia otitidiscaviarum
7.5	-	-	Nocardia iowensis
7.8	-	-	Tsukamurella paurometabola
8	-	-	Mycolicibacterium smegmatis

Cofactor

Cofactor	Comment	Organism
ATP	-	Mycolicibacterium smegmatis
ATP	-	Neurospora crassa
ATP	-	Mycolicibacterium phlei
ATP	-	Nocardia asteroides
ATP	-	Trametes versicolor
ATP	-	Syncephalastrum racemosum
ATP	-	Nocardia otitidiscaviarum
ATP	-	Tsukamurella paurometabola
ATP	-	Nocardia iowensis
ATP	-	Nocardia brasiliensis
ATP	-	Mycobacterium marinum
NADPH	-	Mycolicibacterium smegmatis
NADPH	-	Neurospora crassa
NADPH	-	Mycolicibacterium phlei
NADPH	-	Nocardia asteroides
NADPH	-	Trametes versicolor
NADPH	-	Syncephalastrum racemosum
NADPH	-	Nocardia otitidiscaviarum
NADPH	-	Tsukamurella paurometabola
NADPH	-	Nocardia iowensis
NADPH	-	Nocardia brasiliensis
NADPH	-	Mycobacterium marinum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.143	-	NADP+	pH and temperature not specified in the publication	Mycolicibacterium phlei
0.22	-	diphosphate	pH and temperature not specified in the publication, versus ATP	Mycolicibacterium phlei
0.34	-	diphosphate	pH and temperature not specified in the publication, versus 4-methylbenzoic acid	Mycolicibacterium phlei
8.3	-	AMP	pH and temperature not specified in the publication	Mycolicibacterium phlei

General Information

General Information	Comment	Organism
physiological function	requirement for the presence of a phosphopantetheine transferase for the loading of a phosphopantetheine group onto the CAR enzyme is shown for niCAR. Enzyme CAR prefers substrates in which the carboxylic acid is the only polar or charged group, which gives a useful insight into the substrate specificity of the enzymes. Model development for the prediction of CAR reactivity	Nocardia iowensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.016	-	pyridine-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
0.0166	-	Thiophene-2-carboxylate	pH 7.3, 30°C	Mycolicibacterium phlei
0.025	-	3-Methoxybenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
0.035	-	2-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.054	-	3-Nitrobenzoate	pH 7.5, 30°C	Nocardia iowensis
0.0566	-	Butanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.0635	-	pyridine-2-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.064	-	furan-2-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.068	-	furan-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
0.079	-	3-oxo-3-phenylpropanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.09	-	3-phenylprop-2-ynoate	pH 7.5, 30°C	Nocardia iowensis
0.119	-	3-Phenylpropionate	pH 7.3, 30°C	Mycolicibacterium phlei
0.12	-	benzoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.135	-	Butanoate	pH 7.5, 30°C	Nocardia iowensis
0.183	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.3, 30°C	Mycolicibacterium phlei
0.21	-	3-Nitrobenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.27	-	Butanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.274	-	Butanoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.393	-	3-Nitrobenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.415	-	Thiophene-2-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
0.45	-	4-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.485	-	Octanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.55	-	4-Methylbenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.56	-	3-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.58	-	3-Methoxybenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.6	-	4-nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.62	-	Thiophene-2-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
0.625	-	Thiophene-2-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.689	-	octadecanoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.69	-	4-Methylbenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.7	-	3-Nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.786	-	3-Nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
0.79	-	4-Methoxybenzoate	pH 7.3, 30°C	Mycolicibacterium phlei
0.8	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.86	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.5, 30°C	Nocardia iowensis
0.863	-	3-Phenylpropionate	pH 7.5, 30°C	Nocardia otitidiscaviarum
0.965	-	benzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
1.01	-	Thiophene-2-carboxylate	pH 7.5, 30°C	Nocardia iowensis
1.11	-	3-phenylprop-2-ynoate	pH 7.8, 30°C	Tsukamurella paurometabola
1.185	-	benzoate	pH 7.8, 30°C	Tsukamurella paurometabola
1.28	-	octadecanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
1.34	-	3-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
1.34	-	3-Nitrobenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
1.45	-	benzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
1.47	-	3-Phenylpropionate	pH 7.5, 30°C	Nocardia iowensis
1.57	-	4-Methylbenzoate	pH 7.5, 30°C	Nocardia iowensis
1.59	-	3-oxo-3-phenylpropanoate	pH 7.5, 30°C	Nocardia iowensis
1.61	-	octadecanoate	pH 7.5, 30°C	Nocardia iowensis
1.81	-	benzoate	pH 7.5, 30°C	Nocardia iowensis
1.92	-	4-Methylbenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2	-	benzoate	pH 7.8, 30°C	Tsukamurella paurometabola
2.03	-	(E)-3-phenylprop-2-enoate	pH 7.8, 30°C	Tsukamurella paurometabola
2.09	-	4-Methoxybenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.28	-	3-Methoxybenzoate	pH 7.5, 30°C	Nocardia iowensis
2.35	-	Octanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.431	-	(E)-3-phenylprop-2-enoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.52	-	3-Methoxybenzoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
2.56	-	(E)-3-phenylprop-2-enoate	pH 7.5, 30°C	Nocardia iowensis
2.58	-	3-oxo-3-phenylpropanoate	pH 7.8, 30°C	Tsukamurella paurometabola
3.28	-	4-Methoxybenzoate	pH 7.5, 30°C	Nocardia iowensis
3.617	-	4-nitrobenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
3.621	-	3-oxo-3-phenylpropanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
3.67	-	4-Methylbenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
3.73	-	(E)-3-phenylprop-2-enoate	pH 7.3, 30°C	Mycolicibacterium phlei
4.63	-	3-oxo-3-phenylpropanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
4.82	-	4-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
8.22	-	3-Phenylpropionate	pH 7.8, 30°C	Tsukamurella paurometabola
9	-	2-Methoxybenzoate	pH 7.8, 30°C	Tsukamurella paurometabola
9.15	-	octadecanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
10.22	-	dodecanoate	pH 7.3, 30°C	Mycolicibacterium phlei
11.75	-	trans-2-phenylcyclopropane-1-carboxylate	pH 7.8, 30°C	Tsukamurella paurometabola
11.75	-	dodecanoate	pH 7.5, 30°C	Nocardia otitidiscaviarum
12.5	-	octadecanoate	pH 7.8, 30°C	Tsukamurella paurometabola
15.68	-	4-Methoxybenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
16.06	-	4-Methylbenzoate	pH 7.8, 30°C	Mycolicibacterium smegmatis
18.25	-	Octanoate	pH 7.8, 30°C	Tsukamurella paurometabola
19.19	-	3-Phenylpropionate	pH 8.0, 30°C	Mycolicibacterium smegmatis
19.4	-	Octanoate	pH 7.5, 30°C	Nocardia iowensis
26.27	-	(E)-3-phenylprop-2-enoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
43.6	-	dodecanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
49.3	-	Octanoate	pH 8.0, 30°C	Mycolicibacterium smegmatis
61.67	-	trans-2-phenylcyclopropane-1-carboxylate	pH 8.0, 30°C	Mycolicibacterium smegmatis
65.5	-	dodecanoate	pH 7.8, 30°C	Tsukamurella paurometabola
131	-	dodecanoate	pH 7.5, 30°C	Nocardia iowensis