Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
phenylacetaldehyde | at pH 7.7 and 25°C | Escherichia coli | |
0.5 | - |
NAD+ | at pH 7.7 and 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 3 months, 52% loss of activity | Escherichia coli |
0°C, purified enzyme, 3 months, 46% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chlorophenylacetaldehyde + NAD+ + H2O | - |
Escherichia coli | 4-chlorophenylacetate + NADH + H+ | - |
? | |
4-fluorophenylacetaldehyde + NAD+ + H2O | - |
Escherichia coli | 4-fluorophenylacetate + NADH + H+ | - |
? | |
additional information | the enzyme shows 11% activity with NADP+ compared to NAD+. No activity with phenazine methosulfate | Escherichia coli | ? | - |
? | |
phenylacetaldehyde + NAD+ + H2O | best substrate | Escherichia coli | phenylacetate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FeaB-K-12 | - |
Escherichia coli |
phenylacetaldehyde dehydrogenase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-20 | 55 | after preincubation of the protein at -20 to 45°C, residual activities of 82.7 to 100% are measured. After storage at 50°C, this enzyme shows an activity of about 63.1%, whereas significant enzyme inactivation is observed after incubation at 55°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | most suitable cofactor | Escherichia coli |