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Literature summary for 1.2.1.3 extracted from

  • Brichac, J.; Ho, K.K.; Honzatko, A.; Wang, R.; Lu, X.; Weiner, H.; Picklo, M.J.
    Enantioselective oxidation of trans-4-hydroxy-2-nonenal is aldehyde dehydrogenase isozyme and Mg2+ dependent (2007), Chem. Res. Toxicol., 20, 887-895.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ Mg2+ ions suppresses (R)-trans-4-hydroxy-2-nonenal oxidation by ALDH5A to a greater extent than that of (S)-trans-4-hydroxy-2-nonenal Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
acetaldehyde ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus
0.0007
-
(S)-trans-4-hydroxy-2-nonenal ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus
0.001
-
(R)-trans-4-hydroxy-2-nonenal ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus
5.8
-
acetaldehyde ALDH5A, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ ions stimulate the enantioselective oxidation of (R)-trans-4-hydroxy-2-nonenal by ALDH2 while suppressing (S)-trans-4-hydroxy-2-nonenal oxidation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56500
-
4 * 56500, MALDI-TOF mass spectrometry Rattus norvegicus
226000
-
MALDI-TOF mass spectrometry Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P11884
-
-
Rattus norvegicus P51650
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-trans-4-hydroxy-2-nonenal + NAD+ + H2O
-
Rattus norvegicus (2E,4R)-4-hydroxynon-2-enoic acid + NADH + H+
-
?
(R)-trans-4-hydroxy-2-nonenal + NAD+ + H2O ALDH5A enantioselectively oxidizes (R)-trans-4-hydroxy-2-nonenal Rattus norvegicus (2E,4R)-4-hydroxynon-2-enoic acid + NADH + H+
-
?
(S)-trans-4-hydroxy-2-nonenal + NAD+ + H2O
-
Rattus norvegicus (2E,4S)-4-hydroxynon-2-enoic acid + NADH + H+
-
?
acetaldehyde + NAD+ + H2O
-
Rattus norvegicus acetate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 56500, MALDI-TOF mass spectrometry Rattus norvegicus

Synonyms

Synonyms Comment Organism
ALDH2
-
Rattus norvegicus
ALDH5A
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.039
-
(R)-trans-4-hydroxy-2-nonenal ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus
0.04
-
(S)-trans-4-hydroxy-2-nonenal ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus
0.26
-
acetaldehyde ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus
3.5
-
acetaldehyde ALDH5A, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus