Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ ions suppresses (R)-trans-4-hydroxy-2-nonenal oxidation by ALDH5A to a greater extent than that of (S)-trans-4-hydroxy-2-nonenal | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
acetaldehyde | ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus | |
0.0007 | - |
(S)-trans-4-hydroxy-2-nonenal | ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus | |
0.001 | - |
(R)-trans-4-hydroxy-2-nonenal | ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus | |
5.8 | - |
acetaldehyde | ALDH5A, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ ions stimulate the enantioselective oxidation of (R)-trans-4-hydroxy-2-nonenal by ALDH2 while suppressing (S)-trans-4-hydroxy-2-nonenal oxidation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56500 | - |
4 * 56500, MALDI-TOF mass spectrometry | Rattus norvegicus |
226000 | - |
MALDI-TOF mass spectrometry | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P11884 | - |
- |
Rattus norvegicus | P51650 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-trans-4-hydroxy-2-nonenal + NAD+ + H2O | - |
Rattus norvegicus | (2E,4R)-4-hydroxynon-2-enoic acid + NADH + H+ | - |
? | |
(R)-trans-4-hydroxy-2-nonenal + NAD+ + H2O | ALDH5A enantioselectively oxidizes (R)-trans-4-hydroxy-2-nonenal | Rattus norvegicus | (2E,4R)-4-hydroxynon-2-enoic acid + NADH + H+ | - |
? | |
(S)-trans-4-hydroxy-2-nonenal + NAD+ + H2O | - |
Rattus norvegicus | (2E,4S)-4-hydroxynon-2-enoic acid + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | - |
Rattus norvegicus | acetate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 56500, MALDI-TOF mass spectrometry | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ALDH2 | - |
Rattus norvegicus |
ALDH5A | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
(R)-trans-4-hydroxy-2-nonenal | ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus | |
0.04 | - |
(S)-trans-4-hydroxy-2-nonenal | ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus | |
0.26 | - |
acetaldehyde | ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus | |
3.5 | - |
acetaldehyde | ALDH5A, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus |