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Literature summary for 1.2.1.3 extracted from

  • McLeish, M.J.; Kneen, M.M.; Gopalakrishna, K.N.; Koo, C.W.; Babbitt, P.C.; Gerlt, J.A.; Kenyon, G.L.
    Identification and characterization of a mandelamide hydrolase and an NAD(P)+dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633 (2003), J. Bacteriol., 185, 2451-2456.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli coli strain BL21(DE3)pLysS Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0399
-
benzaldehyde at 25°C and pH 8.5, with NADP+ as the electron acceptor Pseudomonas putida
0.0634
-
benzaldehyde at 25°C and pH 8.5, with NAD+ as the electron acceptor Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47430
-
electrospray mass spectrometry Pseudomonas putida
47430
-
deduced from amino acid sequence Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
strain ATCC 12633
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-200 gel filtration, DEAE-Sepharose anion-exchange column chromatography, and Affi-Gel blue column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + NAD(P)+ + H2O
-
Pseudomonas putida benzoate + NAD(P)H + H+
-
?
additional information the enzyme is only marginally active with acetaldehyde, while longer-chain aliphatic aldehydes are poor substrates Pseudomonas putida ?
-
?

Synonyms

Synonyms Comment Organism
BADH
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0425
-
benzaldehyde at 25°C and pH 8.5, with NADP+ as the electron acceptor Pseudomonas putida
0.137
-
benzaldehyde at 25°C and pH 8.5, with NAD+ as the electron acceptor Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent, the enzyme exhibits greater affinity for NAD+ than NADP+ Pseudomonas putida
NADP+ dependent, the enzyme exhibits greater affinity for NAD+ than NADP+ Pseudomonas putida