Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli coli strain BL21(DE3)pLysS | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0399 | - |
benzaldehyde | at 25°C and pH 8.5, with NADP+ as the electron acceptor | Pseudomonas putida | |
0.0634 | - |
benzaldehyde | at 25°C and pH 8.5, with NAD+ as the electron acceptor | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47430 | - |
electrospray mass spectrometry | Pseudomonas putida |
47430 | - |
deduced from amino acid sequence | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
strain ATCC 12633 | - |
Purification (Comment) | Organism |
---|---|
Sephacryl S-200 gel filtration, DEAE-Sepharose anion-exchange column chromatography, and Affi-Gel blue column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD(P)+ + H2O | - |
Pseudomonas putida | benzoate + NAD(P)H + H+ | - |
? | |
additional information | the enzyme is only marginally active with acetaldehyde, while longer-chain aliphatic aldehydes are poor substrates | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BADH | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0425 | - |
benzaldehyde | at 25°C and pH 8.5, with NADP+ as the electron acceptor | Pseudomonas putida | |
0.137 | - |
benzaldehyde | at 25°C and pH 8.5, with NAD+ as the electron acceptor | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent, the enzyme exhibits greater affinity for NAD+ than NADP+ | Pseudomonas putida | |
NADP+ | dependent, the enzyme exhibits greater affinity for NAD+ than NADP+ | Pseudomonas putida |