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Literature summary for 1.2.1.3 extracted from

  • Ho, K.K.; Hurley, T.D.; Weiner, H.
    Selective alteration of the rate-limiting step in cytosolic aldehyde dehydrogenase through random mutagenesis (2006), Biochemistry, 45, 9445-9453.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
S31T/V63A/T244S/E479D generated by random mutagenesis and selected for insensivity to Mg2+ inhibition Homo sapiens
S33C/T244S/C463S generated by random mutagenesis and selected for insensivity to Mg2+ inhibition Homo sapiens
T244S the T244S mutation is not inhibited by Mg2+. The mutant shows a decreasing Km for NAD+ binding with increasing Mg2+ concentration. chloroacetaldehyde is a better substrate for the mutant enzyme than acetaldehyde in the presence of Mg2+ which is similar to the Mg2+-dependent ALDH2 Homo sapiens
T244S/D391E generated by random mutagenesis and selected for insensivity to Mg2+ inhibition Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 70% inhibition at 0.2 mM; 85% inhibition at 0.5 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
propanal 50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
0.0036
-
NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,0.14 mM propanal, 25°C Homo sapiens
0.0058
-
propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
0.0145
-
NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P05091
-
-

Purification (Commentary)

Purification (Comment) Organism
two chromatography steps, homogeneous on SDS-PAGE Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Homo sapiens acetate + NADH + H+
-
?
chloroacetaldehyde + NAD+ + H2O
-
Homo sapiens chloroacetate + NADH + H+
-
?
propanal + NAD+ + H2O
-
Homo sapiens propionate + NADH
-
?

Synonyms

Synonyms Comment Organism
ALDH1
-
Homo sapiens
class 1 aldehyde dehydrogenase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
propanal 50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
1.48
-
propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens