Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S31T/V63A/T244S/E479D | generated by random mutagenesis and selected for insensivity to Mg2+ inhibition | Homo sapiens |
S33C/T244S/C463S | generated by random mutagenesis and selected for insensivity to Mg2+ inhibition | Homo sapiens |
T244S | the T244S mutation is not inhibited by Mg2+. The mutant shows a decreasing Km for NAD+ binding with increasing Mg2+ concentration. chloroacetaldehyde is a better substrate for the mutant enzyme than acetaldehyde in the presence of Mg2+ which is similar to the Mg2+-dependent ALDH2 | Homo sapiens |
T244S/D391E | generated by random mutagenesis and selected for insensivity to Mg2+ inhibition | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 70% inhibition at 0.2 mM; 85% inhibition at 0.5 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
propanal | 50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,1 mM NAD+, 25°C | Homo sapiens | |
0.0036 | - |
NAD+ | 50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,0.14 mM propanal, 25°C | Homo sapiens | |
0.0058 | - |
propanal | 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C | Homo sapiens | |
0.0145 | - |
NAD+ | 50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05091 | - |
- |
Purification (Comment) | Organism |
---|---|
two chromatography steps, homogeneous on SDS-PAGE | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? | |
chloroacetaldehyde + NAD+ + H2O | - |
Homo sapiens | chloroacetate + NADH + H+ | - |
? | |
propanal + NAD+ + H2O | - |
Homo sapiens | propionate + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH1 | - |
Homo sapiens |
class 1 aldehyde dehydrogenase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
propanal | 50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,1 mM NAD+, 25°C | Homo sapiens | |
1.48 | - |
propanal | 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |