Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | slight activation | Pseudomonas aeruginosa | |
NH4+ | activation seems to be due to increase of ionic strength | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Iodosobenzoate | 0.1 mM, 3 min, complete inhibition | Pseudomonas aeruginosa | |
p-hydroxymercuribenzoate | 0.1 mM, 3 min, complete inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence of pH-value and buffer on Km-value for hexanal | Pseudomonas aeruginosa | |
0.0095 | - |
hexanal | - |
Pseudomonas aeruginosa | |
0.045 | - |
Butanal | - |
Pseudomonas aeruginosa | |
0.064 | - |
propanal | - |
Pseudomonas aeruginosa | |
0.165 | - |
acetaldehyde | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slight activation | Pseudomonas aeruginosa | |
K+ | activation seems to be due to increase of ionic strength | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
225000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | constitutive enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-8°C, 16% glycerol, 1 mM dithiothreitol, 25% loss of activity after 6 months | Pseudomonas aeruginosa |
4°C, 60% loss of activity after 44 h in absence of glycerol, 10% loss of activity in presence of 16% glycerol | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Pseudomonas aeruginosa | acetate + NADH + H+ | - |
? | |
butanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | butyrate + NADH + H+ | - |
? | |
decanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | decanoate + NADH | - |
? | |
hexanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | hexanoate + NADH + H+ | - |
? | |
additional information | constitutive enzyme | Pseudomonas aeruginosa | ? | - |
? | |
octanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | octanoate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | propionate + NADH | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
30°C, stable | Pseudomonas aeruginosa |
8.6 | - |
30°C, rapid inactivation, NAD+ protects | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |