Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 0.1 mM, 10% inhibition of cytosolic enzyme | Oryctolagus cuniculus | |
CsCl2 | 0.1 mM, 19% inhibition of cytosolic enzyme | Oryctolagus cuniculus | |
Cu2+ | 0.1 mM CuSO4, 7% inhibition of microsomal enzyme, 81% inhibition of the cytosolic enzyme | Oryctolagus cuniculus | |
Disulfiram | no effect on microsomal enzyme, complete inhibition of the cytosolic enzyme | Oryctolagus cuniculus | |
KCl | 0.1 mM, 16% inhibition | Oryctolagus cuniculus | |
Mg2+ | 0.1 mM MgCl2, 63% inhibition of cytosolic enzyme | Oryctolagus cuniculus | |
NiCl2 | 0.1 mM, 24% inhibition of cytosolic enzyme | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
dodecanal | microsomal enzyme | Oryctolagus cuniculus | |
0.035 | - |
Heptanal | microsomal enzyme | Oryctolagus cuniculus | |
0.294 | - |
propanal | microsomal enzyme | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 0.1 mM, slight activation of microsomal enzyme | Oryctolagus cuniculus | |
CaCl2 | 0.1 mM, slight activation of microsomal enzyme | Oryctolagus cuniculus | |
CsCl | 0.1 mM, slight activation of microsomal enzyme | Oryctolagus cuniculus | |
Mg2+ | no effect | Oryctolagus cuniculus | |
NaCl | 0.1 mM, slight activation of microsomal enzyme | Oryctolagus cuniculus | |
NiCl2 | 0.1 mM, slight activation of microsomal enzyme | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
formation of large aggregates in absence of detergent | Oryctolagus cuniculus |
60000 | - |
6 * 60000, SDS-PAGE | Oryctolagus cuniculus |
370000 | - |
gel filtration in presence of detergents | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
microsomal enzyme | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.75 | - |
microsomal enzyme | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | activity with cytosolic enzyme, no activity with microsomal enzyme | Oryctolagus cuniculus | acetate + NADH + H+ | - |
? | |
butanal + NAD+ + H2O | n-butanal | Oryctolagus cuniculus | butyrate + NADH + H+ | - |
? | |
decanal + NAD+ + H2O | n-decylaldehyde | Oryctolagus cuniculus | decanoate + NADH | - |
? | |
dodecanal + NAD+ + H2O | n-dodecylaldehyde | Oryctolagus cuniculus | dodecanoate + NADH | - |
? | |
formaldehyde + NAD+ + H2O | activity with cytosolic enzyme, no activity with microsomal enzyme | Oryctolagus cuniculus | formate + NADH | - |
? | |
heptanal + NAD+ + H2O | n-heptanal | Oryctolagus cuniculus | heptanoate + NADH | - |
? | |
hexanal + NAD+ + H2O | n-hexanal | Oryctolagus cuniculus | hexanoate + NADH + H+ | - |
? | |
n-nonanal + NAD+ + H2O | - |
Oryctolagus cuniculus | n-nonanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | n-octylaldehyde | Oryctolagus cuniculus | octanoate + NADH | - |
? | |
pentanal + NAD+ + H2O | n-pentanal | Oryctolagus cuniculus | pentanoate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Oryctolagus cuniculus | propionate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 60000, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
microsomal enzyme | Oryctolagus cuniculus |
9.5 | 10.5 | cytosolic enzyme | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Oryctolagus cuniculus |