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Literature summary for 1.2.1.3 extracted from
- Different types of formaldehyde-oxidizing dehydrogenases in Nocardia species 239: purification and characterization of an NAD-dependent aldehyde dehydrogenase (1990), Arch. Biochem. Biophys., 282, 248-253.
General Stability
| General Stability | Organism |
|---|---|
| freezing-thawing cycles do not affect activity | Nocardia sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | - |
Nocardia sp. |  |
| 1-propanol | - |
Nocardia sp. | |
| acetaldehyde | substrate inhibition | Nocardia sp. | |
| benzaldehyde | substrate inhibition | Nocardia sp. | |
| ethanol | - |
Nocardia sp. | |
| formaldehyde | substrate inhibition | Nocardia sp. | |
| H2O2 | - |
Nocardia sp. | |
| HgCl2 | - |
Nocardia sp. | |
| methanol | - |
Nocardia sp. | |
| PCMB | - |
Nocardia sp. | |
| PMSF | - |
Nocardia sp. | |
| propanal | substrate inhibition | Nocardia sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
propanal | - |
Nocardia sp. | |
| 0.082 | - |
acetaldehyde | - |
Nocardia sp. | |
| 0.33 | - |
benzaldehyde | - |
Nocardia sp. | |
| 0.62 | - |
NAD+ | reaction with formaldehyde | Nocardia sp. | |
| 0.65 | - |
formaldehyde | - |
Nocardia sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
4 * 55000, SDS-PAGE | Nocardia sp. |
| 200000 | - |
non-denaturing PAGE | Nocardia sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardia sp. | - |
239 | - |
| Nocardia sp. 239 | - |
239 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nocardia sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Nocardia sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -40°C, stable for several months | Nocardia sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NAD+ + H2O | - |
Nocardia sp. | acetate + NADH + H+ | - |
? | |
| acetaldehyde + NAD+ + H2O | - |
Nocardia sp. 239 | acetate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Nocardia sp. | benzoate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Nocardia sp. 239 | benzoate + NADH + H+ | - |
? | |
| formaldehyde + NAD+ + H2O | - |
Nocardia sp. | formate + NADH | - |
ir | |
| formaldehyde + NAD+ + H2O | - |
Nocardia sp. 239 | formate + NADH | - |
ir | |
| propanal + NAD+ + H2O | - |
Nocardia sp. | propionate + NADH | - |
? | |
| propanal + NAD+ + H2O | - |
Nocardia sp. 239 | propionate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 55000, SDS-PAGE | Nocardia sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Nocardia sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
- |
Nocardia sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | stable | Nocardia sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADP+ | Nocardia sp. | |
| NAD+ | - |
Nocardia sp. | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
benzaldehyde | - |
Nocardia sp. | |
| 2 | - |
propanal | - |
Nocardia sp. | |
| 5 | - |
acetaldehyde | - |
Nocardia sp. | |
| 6 | - |
formaldehyde | - |
Nocardia sp. | |
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