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Literature summary for 1.2.1.29 extracted from

  • Ong, K.; Liew, S.; Mutalib, S.; Murad, A.; Bakar, F.
    Isolation and cloning of an aryl-aldehyde dehydrogenase gene from the white-rot fungus Pycnoporus cinnabarinus strain MUCL 39533 (2015), Malays. J. Microbiol., 11, 391-397 .
No PubMed abstract available
Search for more literature for 1.2.1.29

Application

Application Comment Organism
synthesis the enzyme can be used for production of bio-vanillin from vanillic acid Trametes cinnabarina

Cloned(Commentary)

Cloned (Comment) Organism
gene PcALDH, cloning from RNA via RT-PCR, sequence comparisons and phylogenetic analysis, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain JM109 Trametes cinnabarina

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information enzyme PcALDH contains a signal peptide Trametes cinnabarina
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vanillin + NAD+ Trametes cinnabarina
-
 vanillinic acid + NADH + H+
-
 r
vanillin + NAD+ Trametes cinnabarina MUCL 39533
-
 vanillinic acid + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Trametes cinnabarina A0A0U2ETT9
-
-
Trametes cinnabarina MUCL 39533 A0A0U2ETT9
-
-

Reaction

Reaction Comment Organism Reaction ID
an aromatic aldehyde + NAD+ + H2O = an aromatic acid + NADH + H+ ALDH catalysis involves acylation and deacylation Trametes cinnabarina
a

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trametes cinnabarina
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues Trametes cinnabarina ?
-
 ?
additional information ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues Trametes cinnabarina MUCL 39533 ?
-
 ?
vanillin + NAD+
-
 Trametes cinnabarina vanillinic acid + NADH + H+
-
 r
vanillin + NAD+
-
 Trametes cinnabarina MUCL 39533 vanillinic acid + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
PcALDH
-
 Trametes cinnabarina

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Trametes cinnabarina
NADH
-
 Trametes cinnabarina

General Information

General Information Comment Organism
evolution in silico analysis of PcALDH indicate that enzyme PcALDH belongs to the ALDH superfamily and class 3 ALDHs Trametes cinnabarina
metabolism in filamentous fungi, vanillin is formed in a two-stage process in which ferulic acid is converted to vanillic acid then reduced to vanillin Trametes cinnabarina
physiological function reduction of vanillic acid to vanillin is catalysed by the key enzyme aryl-aldehyde dehydrogenase Trametes cinnabarina