BRENDA - Enzyme Database
show all sequences of 1.2.1.29

Cloning and heterologous expression of two aryl-aldehyde dehydrogenases from the white-rot basidiomycete Phanerochaete chrysosporium

Nakamura, T.; Ichinose, H.; Wariishi, H.; Biochem. Biophys. Res. Commun. 394, 470-475 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
PcALDH1 and PcALDH2 expressed as C-terminal histidine-tagged proteins in Escherichia coli cells
Phanerochaete chrysosporium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53300
-
PcALDH1, calculated from sequence
Phanerochaete chrysosporium
54400
-
PcALDH2, calculated from sequence
Phanerochaete chrysosporium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
-
white-rot basidiomycete
-
Purification (Commentary)
Commentary
Organism
PcALDH1 and PcALDH2 recombinant protein purified with a His-tag affinity column
Phanerochaete chrysosporium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4,5-trimethoxybenzoic acid + NADH + H+
-
-
-
?
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4,5-trimethoxybenzoic acid + NADH + H+
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4-dihydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4-dihydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4-dimethoxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4-dimethoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzaldehyde i.e. vanillin
711095
Phanerochaete chrysosporium
4-hydroxy-3-methoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
4-hydroxybenzoic acid + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
benzoic acid + NADH + H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Phanerochaete chrysosporium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
11
enzymatic reactions carried out at pH 4.0 show only 5–10% of the maximum activities observed at pH 10.0
Phanerochaete chrysosporium
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Phanerochaete chrysosporium
PcALDH1, estimated from sequence
-
5.9
Phanerochaete chrysosporium
PcALDH2, estimated from sequence
-
6.1
Cloned(Commentary) (protein specific)
Commentary
Organism
PcALDH1 and PcALDH2 expressed as C-terminal histidine-tagged proteins in Escherichia coli cells
Phanerochaete chrysosporium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53300
-
PcALDH1, calculated from sequence
Phanerochaete chrysosporium
54400
-
PcALDH2, calculated from sequence
Phanerochaete chrysosporium
Purification (Commentary) (protein specific)
Commentary
Organism
PcALDH1 and PcALDH2 recombinant protein purified with a His-tag affinity column
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4,5-trimethoxybenzoic acid + NADH + H+
-
-
-
?
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4,5-trimethoxybenzoic acid + NADH + H+
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4-dihydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4-dihydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
3,4-dimethoxybenzoic acid + NADH + H+
-
-
-
?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
3,4-dimethoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium ATCC 34541
4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzaldehyde i.e. vanillin
711095
Phanerochaete chrysosporium
4-hydroxy-3-methoxybenzoic acid + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
4-hydroxybenzoic acid + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
711095
Phanerochaete chrysosporium
benzoic acid + NADH + H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Phanerochaete chrysosporium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
11
enzymatic reactions carried out at pH 4.0 show only 5–10% of the maximum activities observed at pH 10.0
Phanerochaete chrysosporium
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Phanerochaete chrysosporium
PcALDH1, estimated from sequence
-
5.9
Phanerochaete chrysosporium
PcALDH2, estimated from sequence
-
6.1
Expression
Organism
Commentary
Expression
Phanerochaete chrysosporium
up-regulation of PcALDH1 and PcALDH2 in response to exogenous addition of vanillin, which is an abundant key intermediate in the lignin biodegradation process, basidiomycetes presumably respond to vanillin by activating and optimizing ligninolytic processes
up
General Information
General Information
Commentary
Organism
metabolism
results suggest that PcALDHs metabolize aryl-aldehyde compounds generated during fungal degradation of lignin and various aromatic xenobiotics
Phanerochaete chrysosporium
General Information (protein specific)
General Information
Commentary
Organism
metabolism
results suggest that PcALDHs metabolize aryl-aldehyde compounds generated during fungal degradation of lignin and various aromatic xenobiotics
Phanerochaete chrysosporium
Expression (protein specific)
Organism
Commentary
Expression
Phanerochaete chrysosporium
up-regulation of PcALDH1 and PcALDH2 in response to exogenous addition of vanillin, which is an abundant key intermediate in the lignin biodegradation process, basidiomycetes presumably respond to vanillin by activating and optimizing ligninolytic processes
up
Other publictions for EC 1.2.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743201
Ong
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Isolation and cloning of an a ...
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Malay. J. Microbiol.
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1
1
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-
1
-
-
2
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
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-
1
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
711095
Nakamura
Cloning and heterologous expre ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
394
470-475
2010
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
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1
-
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-
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13
-
-
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1
1
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-
-
2
-
-
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1
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-
-
-
-
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-
-
-
2
-
-
-
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1
-
-
-
-
13
-
-
-
-
-
1
1
-
2
1
1
1
1
-
-
288178
Raison
The oxidation of gentisaldehyd ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
118
285-298
1966
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5
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1
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1
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1
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1
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3
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5
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1
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1
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1
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3
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