Cloned (Comment) | Organism |
---|---|
in Escherichia coli DH5[alpha] transformants containing the pSKmsdbsub plasmid | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
methylmalonate semialdehyde dehydrogenase in binary complex with NAD+, showing that the nicotinamide ring is well defined in the electron density due to direct and H2O-mediated hydrogen bonds with the carboxamide and that a conformational isomerization of the NMNH is possible in MSDH | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | further mutant enzyme created by single insertion of glycine at position 225 resulting in significant decrease of the rate constant associated with the dissociation of NADH fromthe NADH/thioacylenzyme complex | Bacillus subtilis |
N427L | substitution dramatically alters the catalytic properties of the enzyme, acylation becomes rate-limiting with a decrease of the associated rate constant by at least 10000fold relative to the wild-type MSDH | Bacillus subtilis |
V229G | significant decrease of the rate constant associated with the dissociation of NADH from the NADH-thioacylenzyme complex | Bacillus subtilis |
V229G | together with insertion of glycine at position 225, significant decrease of the rate constant associated with the dissociation of NADH from the NADH/thioacylenzyme complex | Bacillus subtilis |
V229G/H226P | rate-limiting step changes to acylation instead of deacylation | Bacillus subtilis |
V229G/H226P | together with insertion of glycine at position 225, rate-limiting step changes to acylation instead of deacylation | Bacillus subtilis |
V229G/Y252L/V253I | together with insertion of glycine at position 225, rate-limiting step changes to acylation instead of deacylation | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
methylmalonate semialdehyde | V229G/H226P/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.022 | - |
methylmalonate semialdehyde | G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.027 | - |
methylmalonate semialdehyde | V229G/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.028 | - |
methylmalonate semialdehyde | V229G/H226P, pH 8.2 and 30°C | Bacillus subtilis | |
0.054 | - |
methylmalonate semialdehyde | V229G, pH 8.2 and 30°C | Bacillus subtilis | |
0.06 | - |
methylmalonate semialdehyde | wild type MSDH, pH 8.2 and 30°C | Bacillus subtilis | |
0.063 | - |
CoA | V229G/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.096 | - |
CoA | V229G/H226P/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.119 | - |
CoA | V229G/H226P, pH 8.2 and 30°C | Bacillus subtilis | |
0.12 | - |
CoA | wild type MSDH, pH 8.2 and 30°C | Bacillus subtilis | |
0.15 | - |
NAD+ | V229G/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.151 | - |
CoA | G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.215 | - |
methylmalonate semialdehyde | V229G/Y252L/V253I/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.234 | - |
CoA | V229G, pH 8.2 and 30°C | Bacillus subtilis | |
0.497 | - |
CoA | V229G/Y252L/V253I/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.57 | - |
NAD+ | V229G, pH 8.2 and 30°C | Bacillus subtilis | |
0.66 | - |
NAD+ | V229G/H226P, pH 8.2 and 30°C | Bacillus subtilis | |
0.69 | - |
NAD+ | G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.77 | - |
NAD+ | V229G/H226P/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
2.3 | - |
NAD+ | wild type MSDH, pH 8.2 and 30°C | Bacillus subtilis | |
9.44 | - |
NAD+ | V229G/Y252L/V253I/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P42412 | - |
- |
Purification (Comment) | Organism |
---|---|
MSDH purified by ammonium sulfate fractionation, 40-80%, and gel filtration on a ACA 34 resin | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylmalonate semialdehyde + CoA + H2O + NAD+ | weaker stabilization of the adenine ring triggers early NADH release in MSDH-catalyzed reaction | Bacillus subtilis | propanoyl-CoA + HCO3- + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | dimer of dimers, monomer consists of three domains, the dinucleotide binding domain comprising the residues 3123 and 141251, the catalytic domain with residues 252270, and a small domain, with residues 124140 and 471486 | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
methylmalonate semialdehyde dehydrogenase | - |
Bacillus subtilis |
MSDH | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | V229G/H226P/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.02 | - |
NAD+ | V229G/Y252L/V253I/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.09 | - |
NAD+ | V229G/H226P, pH 8.2 and 30°C | Bacillus subtilis | |
0.21 | - |
NAD+ | V229G/G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.39 | - |
NAD+ | G225 insertion, pH 8.2 and 30°C | Bacillus subtilis | |
0.78 | - |
NAD+ | V229G, pH 8.2 and 30°C | Bacillus subtilis | |
2.2 | - |
NAD+ | wild type MSDH, pH 8.2 and 30°C | Bacillus subtilis |