Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.27 extracted from

  • Sokatch, J.R.; Sanders, L.E.; Marshall, V.P.
    Oxidation of methylmalonate semialdehyde to propionyl coenzyme A in Pseudomonas aeruginosa grown on valine (1968), J. Biol. Chem., 243, 2500-2506.
    View publication on PubMed
Search for more literature for 1.2.1.27

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + NAD+ Pseudomonas aeruginosa
-
 propanoyl-CoA + CO2 + NADH
-
 ir

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + NAD+
-
 Pseudomonas aeruginosa propanoyl-CoA + CO2 + NADH
-
 ir
2-methyl-3-oxopropanoate + CoA + NAD+ other acyl acceptor: 2-mercaptoethanol Pseudomonas aeruginosa propanoyl-CoA + CO2 + NADH
-
 ir
propanal + 2-mercaptoethanol + NAD+
-
 Pseudomonas aeruginosa ? + NADH
-
 ir
propanal + CoA + NAD+
-
 Pseudomonas aeruginosa propanoyl-CoA + NADH
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 2-methyl-3-oxopropanoate + CoA + NAD+ Pseudomonas aeruginosa
8.5
-
 2-methyl-3-oxopropanoate + 2-mercaptoethanol + NAD+ Pseudomonas aeruginosa
8.5
-
 propanal + 2-mercaptoethanol + NAD+ Pseudomonas aeruginosa
9
-
 propanal + CoA + NAD+ Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
7 10 methylmalonate semialdehyde oxidation, more than 90% of maximal activity Pseudomonas aeruginosa
8 10 propanal oxidation, less than 50% of maximal activity below, less than 60% of maximal activity above Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Pseudomonas aeruginosa
NAD+
-
 Pseudomonas aeruginosa