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Literature summary for 1.2.1.26 extracted from

  • Brouns, S.J.; Walther, J.; Snijders, A.P.; van de Werken, H.J.; Willemen, H.L.; Worm, P.; de Vos, M.G.; Andersson, A.; Lundgren, M.; Mazon, H.F.; van den Heuvel, R.H.; Nilsson, P.; Salmon, L.; de Vos, W.M.; Wright, P.C.; Bernander, R.; van der Oost, J.
    Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment (2006), J. Biol. Chem., 281, 27378-27388.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NADP+ the enzyme prefers NADP+ over NAD+ Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52290
-
4 * 52290, calculated from sequence Saccharolobus solfataricus
210100
-
mass spectrometry Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-dioxopentanoate + NADP+ + H2O Saccharolobus solfataricus the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2-oxoglutarate + NADPH + 2 H+
-
?
2,5-dioxopentanoate + NADP+ + H2O Saccharolobus solfataricus P2 the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2-oxoglutarate + NADPH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97UA1
-
-
Saccharolobus solfataricus P2 Q97UA1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dioxopentanoate + NADP+ + H2O
-
Saccharolobus solfataricus 2-oxoglutarate + NADPH + 2 H+
-
?
2,5-dioxopentanoate + NADP+ + H2O the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus 2-oxoglutarate + NADPH + 2 H+
-
?
2,5-dioxopentanoate + NADP+ + H2O
-
Saccharolobus solfataricus P2 2-oxoglutarate + NADPH + 2 H+
-
?
2,5-dioxopentanoate + NADP+ + H2O the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus P2 2-oxoglutarate + NADPH + 2 H+
-
?
DL-glyceraldehyde + NADP+ + H2O
-
Saccharolobus solfataricus glycerate + NADPH + H+
-
?
DL-glyceraldehyde + NADP+ + H2O
-
Saccharolobus solfataricus P2 glycerate + NADPH + H+
-
?
glycolaldehyde + NADP+ + H2O
-
Saccharolobus solfataricus glycolate + NADPH + H+
-
?
glycolaldehyde + NADP+ + H2O
-
Saccharolobus solfataricus P2 glycolate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 52290, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
DopDH
-
Saccharolobus solfataricus
SSO3117
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
86
-
half-life time: 18 min Saccharolobus solfataricus
90
-
half-life time: below 10 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.8
-
DL-glyceraldehyde pH 7.5, 70°C Saccharolobus solfataricus
5.3
-
glycolaldehyde pH 7.5, 70°C Saccharolobus solfataricus
8.6
-
2,5-dioxopentanoate pH 7.5, 70°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharolobus solfataricus
7.8
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
6.7 8.2 more than 50% of maximal activity Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Saccharolobus solfataricus