Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme prefers NADP+ over NAD+ | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52290 | - |
4 * 52290, calculated from sequence | Saccharolobus solfataricus |
210100 | - |
mass spectrometry | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dioxopentanoate + NADP+ + H2O | Saccharolobus solfataricus | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
2,5-dioxopentanoate + NADP+ + H2O | Saccharolobus solfataricus P2 | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2-oxoglutarate + NADPH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97UA1 | - |
- |
Saccharolobus solfataricus P2 | Q97UA1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dioxopentanoate + NADP+ + H2O | - |
Saccharolobus solfataricus | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
2,5-dioxopentanoate + NADP+ + H2O | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
2,5-dioxopentanoate + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
2,5-dioxopentanoate + NADP+ + H2O | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus P2 | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
DL-glyceraldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus | glycerate + NADPH + H+ | - |
? | |
DL-glyceraldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | glycerate + NADPH + H+ | - |
? | |
glycolaldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus | glycolate + NADPH + H+ | - |
? | |
glycolaldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | glycolate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 52290, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
DopDH | - |
Saccharolobus solfataricus |
SSO3117 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
86 | - |
half-life time: 18 min | Saccharolobus solfataricus |
90 | - |
half-life time: below 10 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
DL-glyceraldehyde | pH 7.5, 70°C | Saccharolobus solfataricus | |
5.3 | - |
glycolaldehyde | pH 7.5, 70°C | Saccharolobus solfataricus | |
8.6 | - |
2,5-dioxopentanoate | pH 7.5, 70°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
7.8 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.2 | more than 50% of maximal activity | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharolobus solfataricus |