BRENDA - Enzyme Database show
show all sequences of 1.2.1.26

alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution

Watanabe, S.; Yamada, M.; Ohtsu, I.; Makino, K.; J. Biol. Chem. 282, 6685-6695 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
D-galactarate
induces KGSADH-II
Azospirillum brasilense
D-glucarate
induces KGSADH-II
Azospirillum brasilense
hydroxy-L-proline
induces KGSADH-III
Azospirillum brasilense
L-arabinose
induces KGSADH-I
Azospirillum brasilense
Application
Application
Commentary
Organism
additional information
three different types of KGSADH appear in the bacterial evolutional stage convergently; three different types of KGSADH appear in the bacterial evolutional stage convergently; three different types of KGSADH appear in the bacterial evolutional stage convergently
Azospirillum brasilense
additional information
the ALDH branch including ycbD protein is designated the KGSADH subclass (type III)
Bacillus subtilis
Cloned(Commentary)
Commentary
Organism
expressed as His6-tagged ycbD protein
Bacillus subtilis
KGSADH-III overexpressed as His6-tagged protein in Escherichia coli DH5alpha; KGSADH-II overexpressed as His6-tagged protein in Escherichia coli DH5alpha
Azospirillum brasilense
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
0.0075
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
0.011
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
0.0149
-
NADP+
-
Azospirillum brasilense
0.0246
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
0.0352
-
Octylaldehyde
-
Bacillus subtilis
0.0393
-
heptaldehyde
-
Bacillus subtilis
0.0622
-
NADP+
-
Azospirillum brasilense
0.0814
-
hexylaldehyde
-
Bacillus subtilis
0.094
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Bacillus subtilis
0.1169
-
NAD+
-
Azospirillum brasilense
0.145
-
Valeraldehyde
-
Bacillus subtilis
0.17
-
butylaldehyde
-
Bacillus subtilis
0.2599
-
NAD+
KGSADH-I
Azospirillum brasilense
0.5569
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Bacillus subtilis
0.74
-
NAD+
-
Bacillus subtilis
1.08
-
NADP+
-
Bacillus subtilis
1.136
-
NAD+
-
Azospirillum brasilense
1.46
-
propionaldehyde
-
Bacillus subtilis
2.427
-
NADP+
KGSADH-I
Azospirillum brasilense
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
gel filtration; gel filtration
Azospirillum brasilense
54351
-
2 * 57000 or 2 * 54351, gel filtration
Azospirillum brasilense
55185
-
2 * 57000 or 2 * 55185, gel filtration
Azospirillum brasilense
57000
-
2 * 57000 or 2 * 54351, gel filtration; 2 * 57000 or 2 * 55185, gel filtration
Azospirillum brasilense
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Azospirillum brasilense
Q08IB7
strain ATCC29145
-
Azospirillum brasilense
Q08IC0
strain ATCC29145
-
Azospirillum brasilense
Q1JUP4
strain ATCC29145
-
Azospirillum brasilense ATCC 29145
Q08IB7
strain ATCC29145
-
Azospirillum brasilense ATCC 29145
Q08IC0
strain ATCC29145
-
Azospirillum brasilense ATCC 29145
Q1JUP4
strain ATCC29145
-
Bacillus subtilis
-
-
-
Purification (Commentary)
Commentary
Organism
KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration; KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration
Azospirillum brasilense
Storage Stability
Storage Stability
Organism
-35°C, buffer E, pH 8.0, 250 mM imidazole
Azospirillum brasilense
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
693021
Azospirillum brasilense
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
693021
Azospirillum brasilense ATCC 29145
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
butyraldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-butanoate + NAD(P)H + H+
-
-
-
?
heptaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-heptanoate + NAD(P)H + H+
-
-
-
?
hexylaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-hexanoate + NAD(P)H + H+
-
-
-
?
octylaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-octanoate + NAD(P)H + H+
-
-
-
?
propionaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
propionate + NAD(P)H + H+
-
-
-
?
valeraldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-pentanoate + NAD(P)H + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 57000 or 2 * 54351, gel filtration; 2 * 57000 or 2 * 55185, gel filtration
Azospirillum brasilense
tetramer
gel filtration
Azospirillum brasilense
tetramer
gel filtration
Bacillus subtilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.74
-
NAD+
-
Bacillus subtilis
0.87
-
heptaldehyde
-
Bacillus subtilis
0.97
-
Octylaldehyde
-
Bacillus subtilis
1.08
-
NADP+
-
Bacillus subtilis
1.12
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Bacillus subtilis
1.29
-
propionaldehyde
-
Bacillus subtilis
1.35
-
butylaldehyde
-
Bacillus subtilis
1.47
-
hexylaldehyde
-
Bacillus subtilis
2.1
-
Valeraldehyde
-
Bacillus subtilis
2.75
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Bacillus subtilis
9.86
-
NADP+
KGSADH-I
Azospirillum brasilense
10.2
-
NADP+
-
Azospirillum brasilense
15.3
-
NADP+
-
Azospirillum brasilense
15.4
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
16.7
-
NAD+
KGSADH-I
Azospirillum brasilense
21.3
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
23
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
35.5
-
NAD+
-
Azospirillum brasilense
52.3
-
NAD+
-
Azospirillum brasilense
53.9
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
; no significant preference between NAD+ and NADP+ in activity; prefered cofactor of KGSADH-II
Azospirillum brasilense
NAD+
-
Bacillus subtilis
NADP+
prefered cofactor of KGSADH-III
Azospirillum brasilense
NADP+
-
Bacillus subtilis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
D-galactarate
induces KGSADH-II
Azospirillum brasilense
D-glucarate
induces KGSADH-II
Azospirillum brasilense
hydroxy-L-proline
induces KGSADH-III
Azospirillum brasilense
L-arabinose
induces KGSADH-I
Azospirillum brasilense
Application (protein specific)
Application
Commentary
Organism
additional information
three different types of KGSADH appear in the bacterial evolutional stage convergently
Azospirillum brasilense
additional information
the ALDH branch including ycbD protein is designated the KGSADH subclass (type III)
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed as His6-tagged ycbD protein
Bacillus subtilis
KGSADH-II overexpressed as His6-tagged protein in Escherichia coli DH5alpha
Azospirillum brasilense
KGSADH-III overexpressed as His6-tagged protein in Escherichia coli DH5alpha
Azospirillum brasilense
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
no significant preference between NAD+ and NADP+ in activity
Azospirillum brasilense
NAD+
prefered cofactor of KGSADH-II
Azospirillum brasilense
NAD+
-
Azospirillum brasilense
NAD+
-
Bacillus subtilis
NADP+
-
Azospirillum brasilense
NADP+
prefered cofactor of KGSADH-III
Azospirillum brasilense
NADP+
-
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
0.0075
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
0.011
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
0.0149
-
NADP+
-
Azospirillum brasilense
0.0246
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
0.0352
-
Octylaldehyde
-
Bacillus subtilis
0.0393
-
heptaldehyde
-
Bacillus subtilis
0.0622
-
NADP+
-
Azospirillum brasilense
0.0814
-
hexylaldehyde
-
Bacillus subtilis
0.094
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Bacillus subtilis
0.1169
-
NAD+
-
Azospirillum brasilense
0.145
-
Valeraldehyde
-
Bacillus subtilis
0.17
-
butylaldehyde
-
Bacillus subtilis
0.2599
-
NAD+
KGSADH-I
Azospirillum brasilense
0.5569
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Bacillus subtilis
0.74
-
NAD+
-
Bacillus subtilis
1.08
-
NADP+
-
Bacillus subtilis
1.136
-
NAD+
-
Azospirillum brasilense
1.46
-
propionaldehyde
-
Bacillus subtilis
2.427
-
NADP+
KGSADH-I
Azospirillum brasilense
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
gel filtration
Azospirillum brasilense
54351
-
2 * 57000 or 2 * 54351, gel filtration
Azospirillum brasilense
55185
-
2 * 57000 or 2 * 55185, gel filtration
Azospirillum brasilense
57000
-
2 * 57000 or 2 * 54351, gel filtration
Azospirillum brasilense
57000
-
2 * 57000 or 2 * 55185, gel filtration
Azospirillum brasilense
Purification (Commentary) (protein specific)
Commentary
Organism
KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration
Azospirillum brasilense
KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration
Azospirillum brasilense
Storage Stability (protein specific)
Storage Stability
Organism
-35°C, buffer E, pH 8.0, 250 mM imidazole
Azospirillum brasilense
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
693021
Azospirillum brasilense
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
693021
Azospirillum brasilense ATCC 29145
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
butyraldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-butanoate + NAD(P)H + H+
-
-
-
?
heptaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-heptanoate + NAD(P)H + H+
-
-
-
?
hexylaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-hexanoate + NAD(P)H + H+
-
-
-
?
octylaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-octanoate + NAD(P)H + H+
-
-
-
?
propionaldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
propionate + NAD(P)H + H+
-
-
-
?
valeraldehyde + NAD(P)+ + H2O
-
693021
Bacillus subtilis
n-pentanoate + NAD(P)H + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 57000 or 2 * 54351, gel filtration
Azospirillum brasilense
dimer
2 * 57000 or 2 * 55185, gel filtration
Azospirillum brasilense
tetramer
gel filtration
Azospirillum brasilense
tetramer
gel filtration
Bacillus subtilis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.74
-
NAD+
-
Bacillus subtilis
0.87
-
heptaldehyde
-
Bacillus subtilis
0.97
-
Octylaldehyde
-
Bacillus subtilis
1.08
-
NADP+
-
Bacillus subtilis
1.12
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Bacillus subtilis
1.29
-
propionaldehyde
-
Bacillus subtilis
1.35
-
butylaldehyde
-
Bacillus subtilis
1.47
-
hexylaldehyde
-
Bacillus subtilis
2.1
-
Valeraldehyde
-
Bacillus subtilis
2.75
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Bacillus subtilis
9.86
-
NADP+
KGSADH-I
Azospirillum brasilense
10.2
-
NADP+
-
Azospirillum brasilense
15.3
-
NADP+
-
Azospirillum brasilense
15.4
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
16.7
-
NAD+
KGSADH-I
Azospirillum brasilense
21.3
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
23
-
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
Azospirillum brasilense
35.5
-
NAD+
-
Azospirillum brasilense
52.3
-
NAD+
-
Azospirillum brasilense
53.9
-
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
Azospirillum brasilense
Other publictions for EC 1.2.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715548
Nunn
Metabolism of pentose sugars i ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biol. Chem.
285
33701-33709
2010
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
1
-
-
1
-
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
693064
Aghaie
New Insights into the Alternat ...
Acinetobacter baylyi, Acinetobacter baylyi ADP1, Pseudomonas putida
J. Biol. Chem.
283
15638-15646
2008
-
2
2
-
1
-
1
10
-
-
1
-
-
9
-
-
2
-
-
-
-
-
11
1
-
-
-
10
-
-
-
3
-
-
-
-
2
2
3
-
1
-
-
1
-
10
-
-
1
-
-
-
-
2
-
-
-
-
11
1
-
-
-
10
-
-
-
-
-
-
-
-
-
-
693021
Watanabe
alpha-ketoglutaric semialdehyd ...
Azospirillum brasilense, Azospirillum brasilense ATCC 29145, Bacillus subtilis
J. Biol. Chem.
282
6685-6695
2007
4
2
2
-
-
-
-
20
-
-
4
-
-
9
-
-
1
-
-
-
-
2
13
3
-
-
-
20
-
-
-
4
-
-
-
4
4
3
8
-
-
-
-
-
-
20
-
-
6
-
-
-
-
2
-
-
-
2
13
4
-
-
-
20
-
-
-
-
-
-
-
-
-
-
719837
Brouns
Identification of the missing ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biol. Chem.
281
27378-27388
2006
1
-
1
-
-
-
-
-
-
-
2
2
-
4
-
-
1
-
-
-
-
-
8
1
1
-
2
3
2
1
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
-
-
-
8
1
1
-
2
3
2
1
-
-
-
-
-
-
-
-
33852
Dilworth
-
Pentose metabolism in Rhizobiu ...
Bradyrhizobium japonicum, Bradyrhizobium sp., Rhizobium leguminosarum, Rhizobium sp., Sinorhizobium meliloti
J. Gen. Microbiol.
132
2733-2742
1986
-
-
-
-
-
-
-
-
-
-
-
6
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5607
Schimz
-
Regulation of L-arabinose meta ...
Pseudomonas fluorescens
Biochem. Soc. Trans.
3
1087-1089
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288177
Adams
alpha-Ketoglutaric semialdehyd ...
Pseudomonas sp.
J. Biol. Chem.
242
1802-1814
1967
-
-
-
-
-
-
1
4
-
-
1
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
1
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-