Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxybenzaldehyde | linear dead-end inhibition, competitive versus succinate semialdehyde, uncompetitive versus NAD+ | Aspergillus niger | |
AMP | dead-end inhibition, AMP competes with NAD+ | Aspergillus niger | |
additional information | no product inhibition by succinate | Aspergillus niger | |
N-formylglycine | - |
Aspergillus niger | |
NAD+ | - |
Aspergillus niger | |
NADH | product inhibition, competitive versus NAD+, uncompetitive versus succinate semialdehyde | Aspergillus niger | |
succinate semialdehyde | substrate inhibition, uncompetitive versus NAD+ | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and kinetic mechanism, overview | Aspergillus niger | |
0.028 | - |
succinate semialdehyde | pH 9.0 37°C | Aspergillus niger | |
3.1 | - |
NAD+ | pH 9.0 37°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
native enzyme, gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate semialdehyde + NAD+ + H2O | Aspergillus niger | - |
succinate + NADH + 2 H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | Aspergillus niger NCIM 565 | - |
succinate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A2R4V5 | - |
- |
Aspergillus niger NCIM 565 | A2R4V5 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 9fold from mycelia by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography | Aspergillus niger |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinate semialdehyde + NAD+ + H2O = succinate + NADH + H+ | sequential ordered Bi-Bi reaction mechanism | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | grown on gamma-aminobutyric acid | Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.277 | - |
purified enzyme, pH 9.0, 37°C | Aspergillus niger |
Storage Stability | Organism |
---|---|
-20°C, purified native enzyme, 100 mM diphosphate, pH 8.0, 14 mM 2-mercaptoethanol, 20% glycerol, loss of 50% activity witin 5 days | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate semialdehyde + NAD+ + H2O | - |
Aspergillus niger | succinate + NADH + 2 H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | the enzyme is specific for the substrates | Aspergillus niger | succinate + NADH + 2 H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | - |
Aspergillus niger NCIM 565 | succinate + NADH + 2 H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | the enzyme is specific for the substrates | Aspergillus niger NCIM 565 | succinate + NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 44000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
SSADH | - |
Aspergillus niger |
succinic semialdehyde dehydrogenase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Aspergillus niger | |
NAD+ | absolutely specific for | Aspergillus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Aspergillus niger | |
0.44 | - |
NADH | pH 9.0, 37°C, versus succinate semmialdehyde | Aspergillus niger | |
0.55 | - |
NADH | pH 9.0, 37°C, versus NAD+ | Aspergillus niger | |
2.3 | - |
NAD+ | pH 9.0, 37°C | Aspergillus niger | |
3.37 | - |
4-hydroxybenzaldehyde | pH 9.0, 37°C, versus succinate semmialdehyde | Aspergillus niger | |
3.9 | - |
4-hydroxybenzaldehyde | pH 9.0, 37°C, versus NAD+ | Aspergillus niger | |
22.95 | - |
AMP | pH 9.0, 37°C, versus NAD+ | Aspergillus niger | |
31.76 | - |
AMP | pH 9.0, 37°C, versus succinate semmialdehyde | Aspergillus niger |