Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.24 extracted from

  • Fuhrer, T.; Chen, L.; Sauer, U.; Vitkup, D.
    Computational prediction and experimental verification of the gene encoding the NAD+/NADP+-dependent succinate semialdehyde dehydrogenase in Escherichia coli (2007), J. Bacteriol., 189, 8073-8078.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.24

Activating Compound

Activating Compound Comment Organism Structure
additional information gene yneI is responsible for NAD+/NADP+-SSADH activity. The gene is induced by succinate semialdehyde Escherichia coli

Application

Application Comment Organism
additional information yneI, responsible for NAD+/NADP+-dependent SSADH activity, plays a unique physiological role in the general nitrogen metabolism of Escherichia coli. The yneI gene has an important, but not essential, role during growth on arginine and probably has an essential function during growth on putrescine as the nitrogen source. The yneI-encoded activity functions primarily as a valve to prevent toxic accumulation of succinate semialdehyde Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as His-tagged YneI Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information yneI knockout mutant shows strongly inhibited growth compared to the wild-type Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 YneI protein Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli BW25113
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged YneI dehydrogenase purified from cell extracts with HiTrap affinity columns Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NAD+ + H2O
-
 Escherichia coli succinate + NADH + H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Escherichia coli BW25113 succinate + NADH + H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Escherichia coli succinate + NADPH + H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Escherichia coli BW25113 succinate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
SSADH
-
 Escherichia coli
succinate semialdehyde dehydrogenase
-
 Escherichia coli
YneI dehydrogenase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli
NADP+
-
 Escherichia coli