BRENDA - Enzyme Database show
show all sequences of 1.2.1.23

alpha-Keto aldehyde dehydrogenase, an enzyme that catalyzes the enzymic oxidation of methylglyoxal to pyruvate

Monder, C.; J. Biol. Chem. 242, 4603-4609 (1967)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Barbiturates
-
Ovis aries
cyanide
-
Ovis aries
glycine buffer
complete blockage of the reaction
Ovis aries
hydrazine
1-3 mM
Ovis aries
hydroxylamine
1-3 mM
Ovis aries
N-ethylmaleimide
3 mM
Ovis aries
N-tris(hydroxymethyl)methylglycine
-
Ovis aries
p-chloromercuribenzoate
-
Ovis aries
p-hydroxymercuribenzoate
1-3 mM
Ovis aries
Semicarbazide
1-3 mM
Ovis aries
Sodium bisulfite
1 mM
Ovis aries
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NAD+ + H2O
Ovis aries
ir
pyruvate + NADH + H+
-
Ovis aries
-
methylglyoxal + NAD+ + H2O
Ovis aries
i.e. pyruvaldehyde
pyruvate + NADH + H+
-
Ovis aries
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries
-
-
-
Purification (Commentary)
Commentary
Organism
using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200
Ovis aries
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Ovis aries
-
kidney
-
Ovis aries
-
liver
-
Ovis aries
-
lung
-
Ovis aries
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.571
-
-
Ovis aries
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoaldehydes + NAD+ + H2O
enzyme from sheep or rat liver also active with NADP+, see also EC 1.2.1.49
288146
Ovis aries
2-oxo acids + NADH
-
288146
Ovis aries
?
methylglyoxal + NAD+ + H2O
ir
288146
Ovis aries
pyruvate + NADH + H+
-
288146
Ovis aries
-
methylglyoxal + NAD+ + H2O
i.e. pyruvaldehyde
288146
Ovis aries
pyruvate + NADH + H+
-
288146
Ovis aries
-
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
-
not active below
Ovis aries
9.4
-
activity increases up to, Tris buffer
Ovis aries
10.5
-
activity increases up to, carbonate buffer
Ovis aries
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
3-acetyl-pyridine adenine dinucleotide and thionicotinamide adenine dinucleotide are also effective oxidants
Ovis aries
NAD+
-
Ovis aries
NADP+
enzyme from sheep or rat liver, see also EC 1.2.1.49
Ovis aries
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
3-acetyl-pyridine adenine dinucleotide and thionicotinamide adenine dinucleotide are also effective oxidants
Ovis aries
NAD+
-
Ovis aries
NADP+
enzyme from sheep or rat liver, see also EC 1.2.1.49
Ovis aries
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Barbiturates
-
Ovis aries
cyanide
-
Ovis aries
glycine buffer
complete blockage of the reaction
Ovis aries
hydrazine
1-3 mM
Ovis aries
hydroxylamine
1-3 mM
Ovis aries
N-ethylmaleimide
3 mM
Ovis aries
N-tris(hydroxymethyl)methylglycine
-
Ovis aries
p-chloromercuribenzoate
-
Ovis aries
p-hydroxymercuribenzoate
1-3 mM
Ovis aries
Semicarbazide
1-3 mM
Ovis aries
Sodium bisulfite
1 mM
Ovis aries
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NAD+ + H2O
Ovis aries
ir
pyruvate + NADH + H+
-
Ovis aries
-
methylglyoxal + NAD+ + H2O
Ovis aries
i.e. pyruvaldehyde
pyruvate + NADH + H+
-
Ovis aries
-
Purification (Commentary) (protein specific)
Commentary
Organism
using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200
Ovis aries
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Ovis aries
-
kidney
-
Ovis aries
-
liver
-
Ovis aries
-
lung
-
Ovis aries
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.571
-
-
Ovis aries
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoaldehydes + NAD+ + H2O
enzyme from sheep or rat liver also active with NADP+, see also EC 1.2.1.49
288146
Ovis aries
2-oxo acids + NADH
-
288146
Ovis aries
?
methylglyoxal + NAD+ + H2O
ir
288146
Ovis aries
pyruvate + NADH + H+
-
288146
Ovis aries
-
methylglyoxal + NAD+ + H2O
i.e. pyruvaldehyde
288146
Ovis aries
pyruvate + NADH + H+
-
288146
Ovis aries
-
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
-
not active below
Ovis aries
9.4
-
activity increases up to, Tris buffer
Ovis aries
10.5
-
activity increases up to, carbonate buffer
Ovis aries
Other publictions for EC 1.2.1.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742509
Kwak
NAD+-linked alcohol dehydroge ...
Candida albicans
FEBS Lett.
588
1144-1153
2014
-
-
1
-
1
-
-
2
-
-
-
1
-
2
-
-
-
-
-
-
1
-
3
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
2
-
-
-
-
1
2
2
1
2
2
288147
Fujii
The presence of 2-keto-3-deoxy ...
Homo sapiens
Biochem. Biophys. Res. Commun.
210
852-857
1995
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288148
Bender
Biochemical genetics of methyl ...
Rattus norvegicus
Biochem. Genet.
32
147-154
1994
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288137
Oimomi
Purification of alpha-ketoalde ...
Homo sapiens
Experientia
45
463-466
1989
-
-
-
-
-
-
1
2
-
-
1
2
-
1
-
-
1
-
-
1
1
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
1
2
-
-
-
1
-
1
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288138
Rhee
-
Metabolism of 2-ketoaldehydes ...
Pseudomonas putida
Agric. Biol. Chem.
51
1059-1066
1987
5
-
-
-
-
-
8
2
-
1
2
1
-
1
-
-
1
-
-
-
1
1
3
1
-
-
-
-
1
-
-
2
-
-
-
5
-
-
2
-
-
-
-
8
-
2
-
1
2
1
-
-
-
1
-
-
1
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288139
Saez
-
Glucose formation from methylg ...
Rattus norvegicus
Biochem. Soc. Trans.
13
945-946
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288140
Ray
Purification and characterizat ...
Capra hircus
J. Biol. Chem.
257
10566-10570
1982
1
-
-
-
-
-
3
2
-
-
2
1
-
1
-
-
1
-
-
1
1
-
3
1
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
2
-
-
2
1
-
-
-
1
-
1
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288141
Ray
On the interaction of nucleoti ...
Capra hircus
J. Biol. Chem.
257
10571-10574
1982
2
-
-
-
-
-
5
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288142
Dunkerton
Some observations on the NADP+ ...
Ovis aries
Biochem. J.
153
503-504
1976
2
-
-
-
-
-
-
3
-
-
-
1
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
3
-
-
-
1
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288143
Dunkerton
Purification of 2-oxoaldehyde ...
Ovis aries
Biochem. J.
149
609-617
1975
8
-
-
-
-
-
8
16
-
-
-
1
-
1
-
-
1
-
-
1
1
1
4
-
-
-
-
-
1
1
-
2
-
-
-
8
-
-
2
-
-
-
-
8
-
16
-
-
-
1
-
-
-
1
-
1
1
1
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288144
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
222
668-670
1970
-
-
-
-
-
-
2
2
-
2
1
1
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
2
1
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288145
Jellum
Metabolism of the ketoaldehyde ...
Ovis aries, Rattus norvegicus
Biochim. Biophys. Acta
165
357-363
1968
-
-
-
-
-
-
-
2
-
-
-
4
-
2
-
-
1
-
-
2
-
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
-
4
-
-
-
1
-
2
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288146
Monder
alpha-Keto aldehyde dehydrogen ...
Ovis aries
J. Biol. Chem.
242
4603-4609
1967
-
-
-
-
-
-
11
-
-
-
-
2
-
1
-
-
1
-
-
4
1
-
3
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
3
-
-
-
-
11
-
-
-
-
-
2
-
-
-
1
-
4
1
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-