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Literature summary for 1.2.1.19 extracted from

  • Liu, Y.; Song, Y.; Zeng, S.; Patra, B.; Yuan, L.; Wang, Y.
    Isolation and characterization of a salt stress-responsive betaine aldehyde dehydrogenase in Lycium ruthenicum Murr (2018), Physiol. Plant., 163, 73-87 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene AMADH1, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative real-time PCR enzyme expression analysis Lycium ruthenicum
gene AMADH2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative real-time PCR enzyme expression analysis Lycium ruthenicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Lycium ruthenicum
additional information
-
additional information Michaelis-Menten kinetics, except for LrAMADH1 on 4-aminobutyraldehyde Lycium ruthenicum
0.00394
-
4-Aminobutyraldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
0.0148
-
3-aminopropionaldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
0.0259
-
3-aminopropionaldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
0.0312
-
4-Aminobutyraldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Lycium ruthenicum 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-aminopropionaldehyde + NAD+ + H2O Lycium ruthenicum
-
3-aminopropanoate + NADH + 2 H+
-
?
4-aminobutyraldehyde + NAD+ + H2O Lycium ruthenicum
-
4-aminobutanoate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Lycium ruthenicum A0A1P8VFW6 from Turpan Desert Botanical Garden of the Chinese Academy of Sciences, China
-
Lycium ruthenicum A0A1P8VFW8 from Turpan Desert Botanical Garden of the Chinese Academy of Sciences, China
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme AMADH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lycium ruthenicum
recombinant His-tagged isozyme AMADH2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lycium ruthenicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Lycium ruthenicum
-
additional information tissue specific enzyme expression analysis by quantitative real-time PCR Lycium ruthenicum
-
seedling
-
Lycium ruthenicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aminopropionaldehyde + NAD+ + H2O
-
Lycium ruthenicum 3-aminopropanoate + NADH + 2 H+
-
?
4-aminobutyraldehyde + NAD+ + H2O
-
Lycium ruthenicum 4-aminobutanoate + NADH + 2 H+
-
?
additional information no activity with betaine aldehyde Lycium ruthenicum ?
-
-
additional information the enzyme is also active with betaine aldehyde, EC 1.2.1.8 Lycium ruthenicum ?
-
-

Synonyms

Synonyms Comment Organism
AMADH
-
Lycium ruthenicum
AMADH1
-
Lycium ruthenicum
AMADH2
-
Lycium ruthenicum
aminoaldehyde dehydrogenase
-
Lycium ruthenicum
BADH
-
Lycium ruthenicum
betaine aldehyde dehydrogenase
-
Lycium ruthenicum
More see also EC 1.2.1.8 Lycium ruthenicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Lycium ruthenicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
3-aminopropionaldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
1.13
-
4-Aminobutyraldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
1.32
-
4-Aminobutyraldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
2.38
-
3-aminopropionaldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Lycium ruthenicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lycium ruthenicum

Expression

Organism Comment Expression
Lycium ruthenicum in NaCl treated seedlings, accompanied by elevated GB accumulation, expression of LrAMADH1 is upregulated, indicating response of LrAMADH1 to salt stress in Lycium ruthenicum up

General Information

General Information Comment Organism
metabolism enzyme LrAMADH2 is not involved in glycine betaine biosynthesis Lycium ruthenicum
metabolism in higher plants, glycine betaine (GB) is synthesized by two-step oxidation of choline. The first step is catalyzed by ferredoxin-dependent choline monooxygenase (CMO, EC 1.14.15.7) to produce betaine aldehyde (BAL). BAL is converted to GB by aminoaldehyde dehydrogenase (AMADH, EC 1.2.1.19) containing the activities of betaine aldehyde dehydrogenase (BADH) Lycium ruthenicum
additional information AMADH2 lacks Ile445, that is contained in all enzymes with BADH activity (EC 1.2.1.8) Lycium ruthenicum
additional information for Ile445 containing AMADHs, the existence of Asn290 rather than Thr290 leads to detectable BADH activity Lycium ruthenicum
physiological function isozyme LrAMADH1 shows low betaine aldehyde dehydrogenase activity, but is proved as bona fide BADH, which involves in glycine betaine (GB) synthesis in planta and responds to salt stress in Lycium ruthenicum plants Lycium ruthenicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
42.3
-
4-Aminobutyraldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
67.6
-
3-aminopropionaldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
91.89
-
3-aminopropionaldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum
286.8
-
4-Aminobutyraldehyde pH 8.0, 30°C, recombinant isozyme LrAMADH1 Lycium ruthenicum