Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for GABA production in an engineered strain of Corynebacterium glutamicum produces 5.3 g/l of GABA | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene patD, recombinant expression in Corynebacterium glutamicum strain ATCC 13032 with deleted gabTDP operon and cgmA gene | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a putrescine-producing recombinant Corynebacterium glutamicum strain is converted into a 4-aminobutyric acid, GABA, producing strain by heterologous expression of putrescine transaminase (PatA) and gamma-aminobutyraldehyde dehydrogenase (PatD) genes from Escherichia coli. The resultant strain produces 5.3 g/l of GABA. GABA production is improved further by adjusting the concentration of nitrogen in the culture medium, by avoiding the formation of the by-product N-acetylputrescine and by deletion of the genes for GABA catabolism and GABA re-uptake | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of 4-aminobutyric acid, GABA, to the growth medium hardly affects growth of Corynebacterium glutamicum, since a half-inhibitory concentration of 1.1 M GABA is determined | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanal + NAD+ + H2O | Escherichia coli | - |
4-aminobutanoate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
MG1655 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanal + NAD+ + H2O | - |
Escherichia coli | 4-aminobutanoate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-aminobutyraldehyde dehydrogenase | - |
Escherichia coli |
PatD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | as alternative to GABA production by glutamate decarboxylation, another route for the production of GABA via putrescine is established in Corynebacterium glutamicum | Escherichia coli |