Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | competitive inhibitor with respect to NAD+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0313 | - |
DELTA1-pyrroline | - |
Escherichia coli | |
0.0538 | - |
NAD+ | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for bivalent cations | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
2 * 95000, SDS-PAGE | Escherichia coli |
195000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanal + NAD+ + H2O | Escherichia coli | role in putrescine degradative pathway | 4-aminobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
from putrescine-grown cells, using ammonium sulfate fractionation and column chromatography on Sephacryl S-300, DEAE-Sephacel and Blue-Sepharose CL6B | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanal + NAD+ + H2O | - |
Escherichia coli | 4-aminobutanoate + NADH + H+ | - |
? | |
4-aminobutanal + NAD+ + H2O | role in putrescine degradative pathway | Escherichia coli | 4-aminobutanoate + NADH + H+ | - |
? | |
DELTA1-pyrroline + NAD+ + H2O | - |
Escherichia coli | 4-aminobutanoate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 95000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
increase in dehydrogenase capacity from 25°C to 75°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
35 | up to 5 min, stable | Escherichia coli |
35 | - |
unstable above | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
in the presence of sodium citrate buffer | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | at pH 4.0 and pH 7.0: about 60% of activity maximum | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | absolutely dependent on | Escherichia coli |