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Literature summary for 1.2.1.16 extracted from
- Kinetic characterization and molecular modeling of NAD(P)(+)-dependent succinic semialdehyde dehydrogenase from Bacillus subtilis as an ortholog YneI (2014), J. Microbiol. Biotechnol., 24, 954-958.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Succinic semialdehyde | complete uncompetitive substrate inhibition | Bacillus subtilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
NAD+ | pH not specified in the publication, 30°C | Bacillus subtilis | |
| 0.39 | - |
NADP+ | pH not specified in the publication, 30°C | Bacillus subtilis | |
| 10.36 | - |
succinate semialdehyde | cofactor NAD+, pH not specified in the publication, 30°C | Bacillus subtilis | |
| 20.5 | - |
succinate semialdehyde | cofactor NADP+, pH not specified in the publication, 30°C | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P94428 | - |
- |
| Bacillus subtilis 168 | P94428 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate semialdehyde + NAD+ + H2O | - |
Bacillus subtilis | succinate + NADH + 2 H+ | - |
? | |
| succinate semialdehyde + NAD+ + H2O | - |
Bacillus subtilis 168 | succinate + NADH + 2 H+ | - |
? | |
| succinate semialdehyde + NADP+ + H2O | - |
Bacillus subtilis | succinate + NADPH + 2 H+ | - |
? | |
| succinate semialdehyde + NADP+ + H2O | - |
Bacillus subtilis 168 | succinate + NADPH + 2 H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SSADH | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.26 | - |
NAD+ | pH not specified in the publication, 30°C | Bacillus subtilis | |
| 5.61 | - |
succinate semialdehyde | cofactor NAD+, pH not specified in the publication, 30°C | Bacillus subtilis | |
| 7.24 | - |
succinate semialdehyde | cofactor NADP+, pH not specified in the publication, 30°C | Bacillus subtilis | |
| 9.93 | - |
NADP+ | pH not specified in the publication, 30°C | Bacillus subtilis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | similar values of the catalytic efficiency (kcat/Km) with both NAD+ and NADP+ as cofactors | Bacillus subtilis | |
| NADP+ | similar values of the catalytic efficiency (kcat/Km) with both NAD+ and NADP+ as cofactors | Bacillus subtilis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
succinate semialdehyde | cofactor NADP+, pH not specified in the publication, 30°C | Bacillus subtilis | |
| 0.6 | - |
succinate semialdehyde | cofactor NAD+, pH not specified in the publication, 30°C | Bacillus subtilis | |
| 25.1 | - |
NAD+ | pH not specified in the publication, 30°C | Bacillus subtilis | |
| 25.4 | - |
NADP+ | pH not specified in the publication, 30°C | Bacillus subtilis | |
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