Literature summary for 1.2.1.16 extracted from

Zheng, H.; Beliavsky, A.; Tchigvintsev, A.; Brunzelle, J.S.; Brown, G.; Flick, R.; Evdokimova, E.; Wawrzak, Z.; Mahadevan, R.; Anderson, W.F.; Savchenko, A.; Yakunin, A.F.
Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium (2013), Proteins, 81, 1031-1041.

Search for more literature for 1.2.1.16

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	the enzyme is stimulated (up to 40%) by the addition of dithiothreitol (0.1-0.2 mM)	Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo-form and in complex with NAD+, hanging drop vapor diffusion method, using 2 M sodium formate and 0.1 M sodium acetate (pH 4.6), at 22°C	Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants	Comment	Organism
D426A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
E442A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
F138A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
K160A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
K160E	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
K92A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
N193A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
R145A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
R428A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
S425A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium
W141A	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
succinate semialdehyde	substrate inhibition at concentrations higher than 0.1 mM	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.022	-	succinate semialdehyde	mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.027	-	succinate semialdehyde	wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.043	-	NAD+	wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.047	-	succinate semialdehyde	wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.12	-	succinate semialdehyde	mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.14	-	succinate semialdehyde	mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.15	-	NAD+	mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.22	-	succinate semialdehyde	mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.23	-	succinate semialdehyde	mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.482	-	NADP+	wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.77	-	succinate semialdehyde	mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.4	-	NADP+	mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.49	-	succinate semialdehyde	mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.6	-	succinate semialdehyde	mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
10	-	NAD+	mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
11	-	NAD+	mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
26	-	NADP+	mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
27	-	NADP+	mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	2 * 49000 , predicted from amino acid sequence	Salmonella enterica subsp. enterica serovar Typhimurium
84000	-	gel filtration	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-naphthaldehyde + NAD+ + H2O	Salmonella enterica subsp. enterica serovar Typhimurium	low dehydrogenase activity	2-naphthoate + NADH + H+	-	?
2-naphthaldehyde + NADP+ + H2O	Salmonella enterica subsp. enterica serovar Typhimurium	low dehydrogenase activity	2-naphthoate + NADPH + H+	-	?
decanal + NAD+ + H2O	Salmonella enterica subsp. enterica serovar Typhimurium	low dehydrogenase activity	decanoate + NADH + H+	-	?
decanal + NADP+ + H2O	Salmonella enterica subsp. enterica serovar Typhimurium	low dehydrogenase activity	decanoate + NADPH + H+	-	?
additional information	Salmonella enterica subsp. enterica serovar Typhimurium	the enzyme shows no detectable activity with hexanal or benzaldehyde	?	-	?
succinate semialdehyde + NAD+ + H2O	Salmonella enterica subsp. enterica serovar Typhimurium	-	succinate + NADH + H+	-	?
succinate semialdehyde + NADP+ + H2O	Salmonella enterica subsp. enterica serovar Typhimurium	-	succinate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	Q8ZPI3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex 75 gel filtration	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-naphthaldehyde + NAD+ + H2O	low dehydrogenase activity	Salmonella enterica subsp. enterica serovar Typhimurium	2-naphthoate + NADH + H+	-	?
2-naphthaldehyde + NADP+ + H2O	low dehydrogenase activity	Salmonella enterica subsp. enterica serovar Typhimurium	2-naphthoate + NADPH + H+	-	?
decanal + NAD+ + H2O	low dehydrogenase activity	Salmonella enterica subsp. enterica serovar Typhimurium	decanoate + NADH + H+	-	?
decanal + NADP+ + H2O	low dehydrogenase activity	Salmonella enterica subsp. enterica serovar Typhimurium	decanoate + NADPH + H+	-	?
additional information	the enzyme shows no detectable activity with hexanal or benzaldehyde	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?
succinate semialdehyde + NAD+ + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	succinate + NADH + H+	-	?
succinate semialdehyde + NADP+ + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	succinate + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 49000 , predicted from amino acid sequence	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms	Comment	Organism
NAD(P)+-dependent succinate semialdehyde dehydrogenase	-	Salmonella enterica subsp. enterica serovar Typhimurium
SSADH	-	Salmonella enterica subsp. enterica serovar Typhimurium
YneI	-	Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.3	-	succinate semialdehyde	mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2	8	succinate semialdehyde	mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.1	-	NADP+	mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.6	-	NADP+	mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
14	-	NAD+	mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
21	-	NADP+	mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
26	-	succinate semialdehyde	mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
38	-	NAD+	wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
39	-	NAD+	mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
45	-	succinate semialdehyde	mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
46	-	NADP+	wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
55	-	succinate semialdehyde	wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
66	-	succinate semialdehyde	mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
68	-	succinate semialdehyde	mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
73	-	succinate semialdehyde	mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
80	-	NAD+	mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
83	-	succinate semialdehyde	wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
164	-	succinate semialdehyde	mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	9	-	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	the enzyme can use both NAD+ and NADP+ although affinity to NAD+ is 10times higher	Salmonella enterica subsp. enterica serovar Typhimurium
NADP+	the enzyme can use both NAD+ and NADP+ although affinity to NAD+ is 10times higher	Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.077	-	NADP+	mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.1	-	NADP+	mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
3.8	-	NAD+	mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
7.3	-	NAD+	mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
8.6	-	NADP+	mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
18	-	succinate semialdehyde	mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
28	-	succinate semialdehyde	mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
86	-	succinate semialdehyde	mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
95	-	NADP+	wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
97	-	NAD+	mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
110	-	succinate semialdehyde	mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
130	-	succinate semialdehyde	mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
130	-	succinate semialdehyde	mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
180	-	succinate semialdehyde	wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
200	-	succinate semialdehyde	wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
210	-	succinate semialdehyde	mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
310	-	succinate semialdehyde	mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
880	-	NAD+	wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)