Literature summary for 1.2.1.16 extracted from

de Carvalho, L.P.; Ling, Y.; Shen, C.; Warren, J.D.; Rhee, K.Y.
On the chemical mechanism of succinic semialdehyde dehydrogenase (GabD1) from Mycobacterium tuberculosis (2011), Arch. Biochem. Biophys., 509, 90-99.

Search for more literature for 1.2.1.16

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the enzyme is not influenced by 5 mM EDTA	Mycobacterium tuberculosis
succinate semialdehyde	-	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	succinate semialdehyde	with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
0.0092	-	NAD+	in the presence of 10 mM Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
0.0133	-	succinate semialdehyde	with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
0.0613	-	NADP+	in the absence of Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	-	Mycobacterium tuberculosis
Co2+	-	Mycobacterium tuberculosis
Mg2+	100% activity at 10 mM Mg2+. With Mg2+ there is a 4fold decrease in the Km value for succinate semialdehyde and a 6fold decrease in the Km value for NADP+	Mycobacterium tuberculosis
Mn2+	-	Mycobacterium tuberculosis
additional information	not activated by Cd2+ and Zn2+	Mycobacterium tuberculosis
Ni2+	-	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mycobacterium tuberculosis	other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates	?	-	?
succinate semialdehyde + NAD+ + H2O	Mycobacterium tuberculosis	-	succinate + NADH + H+	-	?
succinate semialdehyde + NADP+ + H2O	Mycobacterium tuberculosis	-	succinate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Oxidation Stability

Oxidation Stability	Organism
there is no significant loss of catalytic activity, after up to 40 min of incubation of isoform GabD1 with 1 mM H2O2 at pH 7.4	Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates	Mycobacterium tuberculosis	?	-	?
succinate semialdehyde + NAD+ + H2O	-	Mycobacterium tuberculosis	succinate + NADH + H+	-	?
succinate semialdehyde + NADP+ + H2O	-	Mycobacterium tuberculosis	succinate + NADPH + H+	-	?

Synonyms

Synonyms	Comment	Organism
GabD1	isoform	Mycobacterium tuberculosis
succinic semialdehyde dehydrogenase	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3	-	NADP+	in the absence of Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
1.9	-	succinate semialdehyde	with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
2.7	-	NAD+	in the presence of 10 mM Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
4.7	-	succinate semialdehyde	with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	10	the enzyme cannot be assayed below pH 5.5, due to precipitation and loss of activity	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	long incubations lead to a modest utilization of NAD+ as substrate	Mycobacterium tuberculosis
NADP+	although long incubations lead to a modest utilization of NAD+ as substrate, NADP+ is the preferred substrate	Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0628	-	succinate semialdehyde	in the absence of Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
140	-	succinate semialdehyde	with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
210	-	NADP+	in the absence of Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
290	-	NAD+	in the presence of 10 mM Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis
1600	-	succinate semialdehyde	with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)