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Literature summary for 1.2.1.16 extracted from

  • Sanchez, M.; Fernandez, J.; Martin, M.; Gibello, A.; Garrido-Pertierra, A.
    Purification and properties of two succinic semialdehyde dehydrogenases from Klebsiella pneumoniae (1989), Biochim. Biophys. Acta, 990, 225-231.
    View publication on PubMed
Search for more literature for 1.2.1.16

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activator and stabilizer Klebsiella pneumoniae
dithiothreitol activation and stabilization, maximum activity at 25 mM Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
NADPH
-
 Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 NAD+ pH 7.8, 30°C Klebsiella pneumoniae
0.04
-
 succinate semialdehyde
-
 Klebsiella pneumoniae
0.054
-
 succinate semialdehyde pH 7.8, 30°C Klebsiella pneumoniae
0.154
-
 NADP+ pH 7.8, 30°C Klebsiella pneumoniae
0.25
-
 NADP+
-
 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information bivalent kations such as Mg2+, Mn2+, Ca2+ or Fe2+ are not required Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 4 * 59000, SDS-PAGE Klebsiella pneumoniae
275000
-
 gel filtration Klebsiella pneumoniae
300000
-
 gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NADP+ + H2O Klebsiella pneumoniae implicated in 4-hydroxyphenylacetic acid and gamma-aminobutyric acid catabolism succinate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae
-
 M5a1 strain and mutant M5a18
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on Sephacryl S-300, DEAE-Sephacel and Sepharose 4B Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
 4-hydroxyphenylacetic acid and glucose used as carbon source, NH3 used as N-source Klebsiella pneumoniae
0.07
-
 succinate used as carbon source, NH3 used as N-source Klebsiella pneumoniae
0.073
-
 nutrient broth used as carbon source, NADP-dependent form Klebsiella pneumoniae
0.075
-
 glycerol used as carbon source, NH3 used as N-source Klebsiella pneumoniae
0.078
-
 glycerol used as carbon source, 4-aminobutyric acid used as N-source Klebsiella pneumoniae
0.08
-
 4-hydroxyphenylacetic acid used as carbon source, NH3 used as N-source Klebsiella pneumoniae
0.085
-
 4-aminobutyric acid used as carbon source, NH3 used as N-source Klebsiella pneumoniae
0.091
-
 4-hydroxyphenylacetic acid, glucose and cAMP used as carbon source, NH3 used as N-source Klebsiella pneumoniae
0.13
-
 4-hydroxyphenylacetic acid and cAMP used as carbon source, NH3 used as N-source Klebsiella pneumoniae
8.5
-
 pH 7.8, 30°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NAD+ + H2O
-
 Klebsiella pneumoniae succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Klebsiella pneumoniae succinate + NADPH + H+
-
 ?
succinate semialdehyde + NADP+ + H2O implicated in 4-hydroxyphenylacetic acid and gamma-aminobutyric acid catabolism Klebsiella pneumoniae succinate + NADPH + H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Klebsiella pneumoniae succinate + NADPH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, SDS-PAGE Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8
-
 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Klebsiella pneumoniae
NADP+ specific for Klebsiella pneumoniae
NADP+ 20% of the activity with NAD+ Klebsiella pneumoniae

Expression

Organism Comment Expression
Klebsiella pneumoniae expression is induced by exogenous succinic semialdehyde additional information