Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.16 extracted from

  • Ramos, F.; El Guezzar, M.; Grenson, M.; Wiame, J.M.
    Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae (1985), Eur. J. Biochem., 149, 401-404.
    View publication on PubMed
Search for more literature for 1.2.1.16

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol reactivation of purified enzyme preparation Saccharomyces cerevisiae
dithiothreitol reactivation of purified enzyme preparation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
succinate semialdehyde concentration above 0.1 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 succinate semialdehyde
-
 Saccharomyces cerevisiae
0.45
-
 NAD+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NADP+ + H2O Saccharomyces cerevisiae pathway for utilization of 4-aminobutyric acid as nitrogen source for Saccharomyces cerevisiae succinate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyanionic resin Polyanion S1-HR5/5 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0017
-
 wild type, value below, NH4+ used as N-source or uga2 mutant unable to grow on 4-aminobutyric acid, glutamate and 4-aminobutyric acid used as N-source Saccharomyces cerevisiae
0.0025
-
 wild type, glutamate used as N-source Saccharomyces cerevisiae
0.0033
-
 uga3 mutant unable to grow on 4-aminobutyric acid, glutamate and 4-aminobutyric acid used as N-source Saccharomyces cerevisiae
0.075
-
 wild type, NH4+ and 4-aminobutyric acid used as N-source Saccharomyces cerevisiae
0.125
-
 uga1 mutant unable to grow on 4-aminobutyric acid, glutamate and 4-aminobutyric acid used as N-source Saccharomyces cerevisiae
0.38
-
 wild type, glutamate and 4-aminobutyric acid used as N-source Saccharomyces cerevisiae
0.5 0.66 wild type, 4-aminobutyric acid used as N-source Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NAD+ + H2O
-
 Saccharomyces cerevisiae succinate + NADH + H+
-
 r
succinate semialdehyde + NADP+ + H2O
-
 Saccharomyces cerevisiae succinate + NADPH + H+
-
 ?
succinate semialdehyde + NADP+ + H2O pathway for utilization of 4-aminobutyric acid as nitrogen source for Saccharomyces cerevisiae Saccharomyces cerevisiae succinate + NADPH + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+ 2.5fold better activity than with NADP+ at 0.2 mM concentration Saccharomyces cerevisiae
NADP+
-
 Saccharomyces cerevisiae