Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.15 extracted from

  • Wilding, M.; Scott, C.; Peat, T.S.; Newman, J.
    X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC (2017), Acta Crystallogr. Sect. F, 73, 24-28 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene KES23460, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, method screening, crystallization of 20 mg/ml protein with crystalliztaion solution consisting of 1.38 M ammonium sulfate, 3.35% 2,2,2-trifluoroethanol, 2% benzamidine, the resulting crystals are used for microseding with 5 mg/ml protein, 1 mM acetyl-CoA, and a solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, at 8°C, method optimization, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement using structure with PDB ID 4zz7 as the search model Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. AAC
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity Pseudomonas sp.

Subunits

Subunits Comment Organism
hexamer
-
Pseudomonas sp.

Synonyms

Synonyms Comment Organism
KES23460
-
Pseudomonas sp.
NAD-dependent malonate-semialdehyde dehydrogenase
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp.

General Information

General Information Comment Organism
physiological function the enzyme is involved in the beta-alanine catabolism to produce acetyl-CoA Pseudomonas sp.