Cloned (Comment) | Organism |
---|---|
gene KES23460, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, method screening, crystallization of 20 mg/ml protein with crystalliztaion solution consisting of 1.38 M ammonium sulfate, 3.35% 2,2,2-trifluoroethanol, 2% benzamidine, the resulting crystals are used for microseding with 5 mg/ml protein, 1 mM acetyl-CoA, and a solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, at 8°C, method optimization, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement using structure with PDB ID 4zz7 as the search model | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. AAC | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity | Pseudomonas sp. |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
KES23460 | - |
Pseudomonas sp. |
NAD-dependent malonate-semialdehyde dehydrogenase | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the beta-alanine catabolism to produce acetyl-CoA | Pseudomonas sp. |