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Literature summary for 1.2.1.13 extracted from

  • Corpas, F.J.; Gonzalez-Gordo, S.; Palma, J.M.
    Nitric oxide and hydrogen sulfide modulate the NADPH-generating enzymatic system in higher plants (2021), J. Exp. Bot., 72, 830-847 .
    View publication on PubMed
Search for more literature for 1.2.1.13

Cloned(Commentary)

Cloned (Comment) Organism
gene GAPA1 Arabidopsis thaliana
gene GAPA2 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
NaHS
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Arabidopsis thaliana
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P25856
-
-
Arabidopsis thaliana Q9LPW0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Arabidopsis thaliana 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 36300, about, isozyme GAPA1, sequence calculation Arabidopsis thaliana
? x * 37700, about, isozyme GAPA2, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At1g12900
-
 Arabidopsis thaliana
At3g26650
-
 Arabidopsis thaliana
GAPA1
-
 Arabidopsis thaliana
GAPA2
-
 Arabidopsis thaliana
NADP-dependent GAPDH
-
 Arabidopsis thaliana
NADP-GAPDH
-
 Arabidopsis thaliana
NADP-glyceraldehyde-3-phosphate dehydrogenase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana sequence calculation, isozyme GAPA1
-
 6.67
Arabidopsis thaliana sequence calculation, isozyme GAPA2
-
 7.79

General Information

General Information Comment Organism
malfunction several GAPDH isozymes are targets of persulfidation, including NAD- and NADP-dependent GAPDH (GAPC1, GAPC2, GAPA1, and ALDH11A3). The in vitro assay of Arabidopsis leaf extracts in the presence of NaHS show an activity increase of 60% which is reversed by DTT Arabidopsis thaliana
metabolism posttranslational modifications promoted by NO (tyrosine nitration and S-nitrosation), H2S (persulfidation), and glutathione (glutathionylation), affect the cellular redox status through regulation of the NADP-dependent dehydrogenases. Major regulatory mechanisms of the NADPH-generating enzymes by NO and H2S in higher plants, with particular focus on the PTMs mediated by reactive nitrogen species (RNS) and and reactive sulfide species (RSS), overview Arabidopsis thaliana
metabolism posttranslational modifications promoted by NO (tyrosine nitration and S-nitrosation), H2S (persulfidation), and glutathione (glutathionylation), affect the cellular redox status through regulation of the NADP-dependent dehydrogenases. Major regulatory mechanisms of the NADPH-generating enzymes by NO and H2S in higher plants, with particular focus on the PTMs mediated by reactive nitrogen species (RNS) and reactive sulfide species (RSS), overview Arabidopsis thaliana
physiological function the enzyme has different isozymes located in diverse subcellular compartments (chloroplasts, cytosol, mitochondria, and peroxisomes) which contribute to the NAPDH cellular pool. The NADPH/NADP+ ratio is a key indicator of cellular redox status. It is also a cofactor involved in many anabolic pathways and it supports some mechanisms of defense involved in ROS and RNS metabolism. The NADPH pool in cellular compartments is provided by several families of NADP-DHs located in different compartments which guarantee the stability and functioning of the cells. Regulation mechanisms, overview Arabidopsis thaliana