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Literature summary for 1.2.1.13 extracted from
- Purification and characterization of D-glyceraldehyde-3-phosphate dehydrogenase from the thermophilic archaebacterium Methanothermus fervidus (1987), Eur. J. Biochem., 165, 147-155.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Methanothermus fervidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
NADPH | - |
Methanothermus fervidus |  |
| 0.003 | - |
NADP+ | - |
Methanothermus fervidus | |
| 0.066 | - |
NADH | - |
Methanothermus fervidus | |
| 0.65 | - |
NAD+ | - |
Methanothermus fervidus | |
| 43 | - |
arsenate | - |
Methanothermus fervidus | |
| 53 | - |
phosphate | - |
Methanothermus fervidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000, SDS-PAGE | Methanothermus fervidus |
| 200000 | - |
gel filtration | Methanothermus fervidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermus fervidus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermus fervidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49.59 | - |
- |
Methanothermus fervidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NADP+ | - |
Methanothermus fervidus | 3-phospho-D-glyceroyl arsenate + NADPH | - |
? | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Methanothermus fervidus | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
| D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Methanothermus fervidus | 3-phospho-D-glyceroyl phosphate + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 45000, SDS-PAGE | Methanothermus fervidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the efficiency of salts stabilizing against thermal inactivation at a specific ionic strength decrease in the order: potassium phosphate, sodium phosphate, potassium sulfate, sodium sulfate, sodium citrate, potassium chloride, sodium chloride | Methanothermus fervidus |
| 75 | - |
stable up to | Methanothermus fervidus |
| 90 | - |
stabilized by NADP+ and at high ionic strength up to 90°C | Methanothermus fervidus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | cofactor | Methanothermus fervidus | |
| NADP+ | cofactor | Methanothermus fervidus | |
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