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Literature summary for 1.2.1.13 extracted from

  • Zwickl, P.; Fabry, S.; Bogedain, C.; Haas, A.; Hensel, R.
    Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli (1990), J. Bacteriol., 172, 4329-4338.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Pyrococcus woesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADP+
-
Pyrococcus woesei
1
-
NAD+
-
Pyrococcus woesei
40
-
phosphate
-
Pyrococcus woesei
120
-
arsenate
-
Pyrococcus woesei

Organism

Organism UniProt Comment Textmining
Pyrococcus woesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus woesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pyrococcus woesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
Pyrococcus woesei 3-phospho-D-glyceroyl arsenate + NADPH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Pyrococcus woesei 3-phospho-D-glyceroyl phosphate + NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
Pyrococcus woesei 3-phospho-D-glyceroyl phosphate + NADPH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
sodium chloride, potassium chloride, sodium sulfate, potassium sulfate, sodium phosphate, potassium phosphate, sodium citrate and potassium citrate stabilize against thermal inactivation at 104°C. K+ is more effective than Na+ with respect to anion Pyrococcus woesei
97
-
half-life: 65 min Pyrococcus woesei
100
-
half-life: 44 min Pyrococcus woesei
104
-
half-life: 9 min without stabilizer, stable for 30 min in presence of 250 mM potassium citrate Pyrococcus woesei

Cofactor

Cofactor Comment Organism Structure
NAD+ cofactor Pyrococcus woesei
NADP+ cofactor Pyrococcus woesei