Cloned (Comment) | Organism |
---|---|
gene gapdh, recombinant expression in Escherichia coli | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant expression of Gapdh from Thermoanaerobacterium saccharolyticum in Clostridium thermocellum improves the growth rates of the cells in presence of ethanol compared to wild-type | Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | wild-type Clostridium thermocellum is unable to initiate growth when inoculated into medium containing ethanol at concentrations of 20 g/l or higher. Strains adapted for improved tolerance by serial transfer over a period of several weeks have been shown to initiate growth in the presence of 50-55 g/l ethanol. A dramatic accumulation of NADH and NADPH is observed when ethanol is added to the culture. The Gapdh from Clostridium thermocellum (Ctherm_Gapdh) is very sensitive to the NADH/NAD+ ratio | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Acetivibrio thermocellus | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
Clostridium thermocellum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Acetivibrio thermocellus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Clo1313_2095 | - |
Acetivibrio thermocellus |
Ctherm_Gapdh | - |
Acetivibrio thermocellus |
GAPDH | - |
Acetivibrio thermocellus |
glyceraldehyde 3-phosphate dehydrogenase | - |
Acetivibrio thermocellus |
glyceraldehyde-3-phosphate dehydrogenase, type I | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acetivibrio thermocellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
additional information | comparison of Gapdh protein from Clostridium thermocellum and Thermoanaerobacterium saccharolyticum, homology modeling, overview. The Gapdh from Thermoanaerobacterium saccharolyticum is less sensitive to ethanol and the NAD+/NADH ratio. Recombinant Gapdh from Thermoanaerobacterium saccharolyticum expressed in Clostridium thermocellum cells can improve the growth rate and ethanol resistance | Acetivibrio thermocellus |