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Literature summary for 1.2.1.12 extracted from

  • Tian, L.; Perot, S.; Stevenson, D.; Jacobson, T.; Lanahan, A.; Amador-Noguez, D.; Olson, D.; Lynd, L.
    Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol (2017), Biotechnol. Biofuels, 10, 276 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene gapdh, recombinant expression in Escherichia coli Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
additional information recombinant expression of Gapdh from Thermoanaerobacterium saccharolyticum in Clostridium thermocellum improves the growth rates of the cells in presence of ethanol compared to wild-type Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
additional information wild-type Clostridium thermocellum is unable to initiate growth when inoculated into medium containing ethanol at concentrations of 20 g/l or higher. Strains adapted for improved tolerance by serial transfer over a period of several weeks have been shown to initiate growth in the presence of 50-55 g/l ethanol. A dramatic accumulation of NADH and NADPH is observed when ethanol is added to the culture. The Gapdh from Clostridium thermocellum (Ctherm_Gapdh) is very sensitive to the NADH/NAD+ ratio Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Acetivibrio thermocellus
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
Clostridium thermocellum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Acetivibrio thermocellus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
Clo1313_2095
-
Acetivibrio thermocellus
Ctherm_Gapdh
-
Acetivibrio thermocellus
GAPDH
-
Acetivibrio thermocellus
glyceraldehyde 3-phosphate dehydrogenase
-
Acetivibrio thermocellus
glyceraldehyde-3-phosphate dehydrogenase, type I
-
Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Acetivibrio thermocellus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Acetivibrio thermocellus

General Information

General Information Comment Organism
additional information comparison of Gapdh protein from Clostridium thermocellum and Thermoanaerobacterium saccharolyticum, homology modeling, overview. The Gapdh from Thermoanaerobacterium saccharolyticum is less sensitive to ethanol and the NAD+/NADH ratio. Recombinant Gapdh from Thermoanaerobacterium saccharolyticum expressed in Clostridium thermocellum cells can improve the growth rate and ethanol resistance Acetivibrio thermocellus