Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.366 | - |
NAD+ | pH 8.6, temperature not specified in the publication | Bacillus anthracis | |
0.366 | - |
NAD+ | at pH 8.5, temperature not specified in the publication | Bacillus anthracis | |
1.78 | - |
3-phospho-D-glyceroyl phosphate | pH 8.6, temperature not specified in the publication | Bacillus anthracis | |
1.78 | - |
D-glyceraldehyde 3-phosphate | at pH 8.5, temperature not specified in the publication | Bacillus anthracis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Bacillus anthracis | 9986 | - |
extracellular | - |
Bacillus anthracis | - |
- |
membrane | - |
Bacillus anthracis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Bacillus anthracis | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81X74 | - |
- |
Bacillus anthracis | YP_027084 | isoform GapA | - |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA affinity column chromatography | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH | - |
Bacillus anthracis | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Bacillus anthracis | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
GapA | - |
Bacillus anthracis |
GapA | isoform | Bacillus anthracis |
GAPDH | - |
Bacillus anthracis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | - |
Bacillus anthracis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, no loss of activity | Bacillus anthracis |
40 | - |
the enzymatic activity is retained after incubation at 40°C for 30 min | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus anthracis | |
NAD+ | isoform GapA is strictly dependent on NAD+ | Bacillus anthracis |
General Information | Comment | Organism |
---|---|---|
physiological function | human plasminogen predominantly interacts with the GapA isoform at physiological concentrations and the interaction is lysine dependent | Bacillus anthracis |