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Literature summary for 1.2.1.12 extracted from

  • Thanonkeo, P.; Monkeang, R.; Saksirirat, W.; Thanonkeo, S.; Akiyama, K.
    Cloning and molecular characterization of glyceraldehyde-3-phosphate dehydrogenase gene from thermotolerant mushroom, Lentinus polychrous (2010), Afr. J. Biotechnol., 9, 3242-3251.
No PubMed abstract available
Search for more literature for 1.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Lentinus polychrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36200
-
 calculated from amino acid sequence Lentinus polychrous

Organism

Organism UniProt Comment Textmining
Lentinus polychrous D3K1B6 commonly known as hed kradang
-
Lentinus polychrous KPM3 D3K1B6 commonly known as hed kradang
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Lentinus polychrous
-
mycelium
-
 Lentinus polychrous
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Lentinus polychrous 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Lentinus polychrous KPM3 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Synonyms

Synonyms Comment Organism
GAPDH
-
 Lentinus polychrous

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Lentinus polychrous

pI Value

Organism Comment pI Value Maximum pI Value
Lentinus polychrous calculated from amino acid sequence
-
 5.6

Expression

Organism Comment Expression
Lentinus polychrous heat (45°C) and cold (10°C) treatments for 6 h increase the expression level of GAPDH gene approximately 6fold, whereas ethanol (6% (v/v)) and salt (1 M) treatments for 6 h increase the expression of the GAPDH gene about 3fold, as compared to the control condition up

General Information

General Information Comment Organism
physiological function the GAPDH gene product is a heat shock protein which might be involved in the developmental phase of the Lentinus polychrous Lentinus polychrous