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Literature summary for 1.2.1.12 extracted from

  • Too, W.C.; Liew, Y.C.; Few, L.L.
    Cloning of glyceraldehyde-3-phosphate dehydrogenase from an Antarctic psychrophilic bacterium by inverse and splinkerette PCR (2008), J. Basic Microbiol., 48, 430-435.
    View publication on PubMed
Search for more literature for 1.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp. phi9

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36100
-
 x * 36100, calculated Pseudomonas sp. phi9

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. phi9 A7LHM7 antarctic psychrophilic bacterium
-
Pseudomonas sp. phi9 pi9 A7LHM7 antarctic psychrophilic bacterium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
 23°C, pH 7.5, recombinant His-tagged enzyme Pseudomonas sp. phi9

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pseudomonas sp. phi9 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pseudomonas sp. phi9 pi9 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 36100, calculated Pseudomonas sp. phi9