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Literature summary for 1.2.1.12 extracted from
- Inactivation of glyceraldehyde-3-phosphate dehydrogenase by fumarate in diabetes: formation of S-(2-succinyl)cysteine, a novel chemical modification of protein and possible biomarker of mitochondrial stress (2008), Diabetes, 57, 41-49.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | approximately 20% of GAPDH activity is lost by incubation with 0.5 mM fumarate for 24 h, and 100% activity is lost in incubations with 500 mM fumarate at 24 h, NADH in the presence or absence of D-glyceraldehyde 3-phosphate significantly accelerates the inactivation of GAPDH by fumarate | Oryctolagus cuniculus |  |
| fumarate | inactivation of GAPDH by fumarate in vitro correlates with formation of S-(2-succinyl)cysteine, in diabetic compared with control rats fumarate and S-(2-succinyl)cysteine concentration increase approximately 5fold, accompanied by an about 25% decrease in GAPDH specific activity | Rattus norvegicus | |
| additional information | succinate has no effect on enzyme activity | Oryctolagus cuniculus | |
| N-ethylmaleimide | 1 mM completely inactivates the enzyme in 10 min | Oryctolagus cuniculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| radiommunoprecipitation with protein agarose A/G bead | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Rattus norvegicus | - |
| muscle | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Rattus norvegicus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-glyceraldehyde-3-phosphate dehydrogenase | - |
Rattus norvegicus |
| D-glyceraldehyde-3-phosphate dehydrogenase | - |
Oryctolagus cuniculus |
| GAPDH | - |
Rattus norvegicus |
| GAPDH | - |
Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rattus norvegicus | |
| NAD+ | - |
Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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