Any feedback?
Literature summary for 1.2.1.12 extracted from
- Investigation of glyceraldehyde-3-phosphate dehydrogenase from human sperms (2008), Biochemistry, 73, 185-191.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
NAD+ | in 50 mM glycine, 50 mM potassium phosphate, 5 mM EDTA, pH 9.0 | Homo sapiens |  |
| 0.77 | - |
D-glyceraldehyde 3-phosphate | in 50 mM glycine, 50 mM potassium phosphate, 5 mM EDTA, pH 9.0 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, N-terminally truncated enzyme after trypsin treatment, SDS-PAGE | Homo sapiens |
| 58000 | - |
full length subunit | Homo sapiens |
| 150000 | - |
N-terminally truncated enzyme after trypsin-treatment, Blue Native PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O14556 | - |
- |
| Homo sapiens | P04406 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl Sepharose column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| sperm | GAPDS exists in sperms bound to the fibrous sheath of the flagellum | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 40000, N-terminally truncated enzyme after trypsin treatment, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAPDH | - |
Homo sapiens |
| GAPDS | sperm-specific GADPH | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Homo sapiens |
| 8 | 8.3 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
