Literature summary for 1.2.1.12 extracted from

Robien, M.A.; Bosch, J.; Buckner, F.S.; Van Voorhis, W.C.; Worthey, E.A.; Myler, P.; Mehlin, C.; Boni, E.E.; Kalyuzhniy, O.; Anderson, L.; Lauricella, A.; Gulde, S.; Luft, J.R.; DeTitta, G.; Caruthers, J.M.; Hodgson, K.O.; Soltis, M.; Zucker, F.; Verlinde, C.L.; Merritt, E.A.; Schoenfeld, L.W.; Hol, W.G.
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site (2006), Proteins, 62, 570-577.

Application

Application	Comment	Organism
medicine	crystallography may provide structural detail for the development of lead compounds and drug design of novel antimalarials	Plasmodium falciparum

Cloned (Comment)	Organism
into the pET14b vector for expression in Escherichia coli BL21 Star cells	Plasmodium falciparum

Crystallization (Comment)	Organism
the crystal structure is solved at 2.25 A resolution	Plasmodium falciparum

Inhibitors	Comment	Organism	Structure
ferriprotoporphorin IX	-	Plasmodium falciparum

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Plasmodium falciparum	5737	-

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36600	-	monomer	Plasmodium falciparum

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	Plasmodium falciparum	-	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q8IKK7	-	-

Purification (Comment)	Organism
purified on Superflow NTA nickel resin and gel chromatography, Superdex 75 26/60	Plasmodium falciparum

Storage Stability	Organism
-80°C, HEPES buffer, pH 7.25, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% sodium azide	Plasmodium falciparum

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Plasmodium falciparum	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Subunits	Comment	Organism
tetramer	-	Plasmodium falciparum

Synonyms	Comment	Organism
D-glyceraldehyde-3-phosphate dehydrogenase	-	Plasmodium falciparum
glyceraldehyde-3-phosphate dehydrogenase	-	Plasmodium falciparum
PfGAPDH	-	Plasmodium falciparum

Cofactor	Comment	Organism	Structure
NADH	-	Plasmodium falciparum

Organism	Comment	pI Value Maximum	pI Value
Plasmodium falciparum	calculated	-	7.6