Literature summary for 1.2.1.12 extracted from

Ferreira-da-Silva, F.; Pereira, P.J.; Gales, L.; Roessle, M.; Svergun, D.I.; Moradas-Ferreira, P.; Damas, A.M.
The crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures (2006), J. Biol. Chem., 281, 33433-33440.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloned into the pQE32 vector for expression in Escherichia coli M15 cells	Kluyveromyces marxianus

Crystallization (Commentary)

Crystallization (Comment)	Organism
diffraction data to 2.3 A resolution are collected	Kluyveromyces marxianus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	associated with the cell wall	Kluyveromyces marxianus	5618	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
130000	-	tetramer, determined by gel filtration	Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	Kluyveromyces marxianus	-	3-phospho-D-glyceroyl phosphate + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Kluyveromyces marxianus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	N-glycosylated	Kluyveromyces marxianus

Purification (Commentary)

Purification (Comment)	Organism
using a nickel-nitrilo-triacetic acid-agarose column	Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Kluyveromyces marxianus	3-phospho-D-glyceroyl phosphate + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
dimer	the final crystallographic model consists of a dimer	Kluyveromyces marxianus
tetramer	determined by gel filtration	Kluyveromyces marxianus

Synonyms

Synonyms	Comment	Organism
GAPDH	-	Kluyveromyces marxianus
glyceraldehyde-3-phosphate dehydrogenase	-	Kluyveromyces marxianus
kmGAPDH1p	formerly referred to as p37	Kluyveromyces marxianus

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Kluyveromyces marxianus

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Release 2023.1 (January 2023)